An Antisense Circular RNA Regulates Expression of RuBisCO Small Subunit Genes in .

Circular RNA (circRNA) is a novel class of endogenous long non-coding RNA (lncRNA) and participates in diverse physiological process in plants. From the dataset obtained by high-throughput RNA sequencing, we identified a circRNA encoded by the sense strand of the exon regions spanning two RuBisCO small subunit genes, and , in . We further applied the single specific primer-polymerase chain reaction (PCR) and Sanger sequencing techniques to verify this circRNA and named it ( RNA ). Using quantitative real-time PCR (qRT-PCR), we found that shares a similar rhythmic expression pattern with other genes. The expression level of is 10-40 times lower than the expression levels of genes in the photosynthetic organs in , whereas the root lacked expression. Furthermore, we used the delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) to deliver synthesized into seedlings. Our results indicate that could significantly depress the expression of . Given that was expressed at low concentration , we suggest that may represent a fine-tuning mechanism to regulating the expression of genes and protein content in .