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http://dx.doi.org/10.1002/ctm2.423 | DOI Listing |
Angew Chem Int Ed Engl
September 2025
State Key Laboratory of Chemo and Biosensing, School of Biomedical Sciences, Hunan University, Changsha, 410082, China.
Activity-based ubiquitin probes (Ub-ABPs) are powerful tools for studying the functional landscape of deubiquitinases (DUBs). While most existing Ub probes have focused on examining the native state of DUBs, oxidative stress, especially in cancer and inflammatory contexts, can oxidize the catalytic cysteine of DUBs, significantly altering their activity. Here, we developed three novel ubiquitin-based activity probes (Ub-ABPs) to selectively trap the sulfenylated form of deubiquitinases (DUB-SOH).
View Article and Find Full Text PDFMicrobes Infect
September 2025
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530007, P.R. China. Electronic address:
Background: While autophagy is pivotal in antimicrobial defense, its regulatory role in Talaromyces marneffei (TM) infected bronchial epithelium remains elusive.
Objective: To elucidate the impact of TM infection on autophagy in bronchial epithelial cells and to identify the key molecular regulators involved in this process.
Methods: Primary computational screening identified core autophagy modulators.
Food Chem
September 2025
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China. Electronic address:
This study establishes diethyl maleate (DEM) as a novel physiologically relevant oxidative stress model for meat science, uniquely recapitulating gradual glutathione depletion during natural spoilage. Using quantitative proteomics and biochemical analyses (0-48 h postmortem), we demonstrate that DEM-induced stress paradoxically enhances beef colour stability despite accelerated glycolysis (pH 5.6 ± 0.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
September 2025
School of Life Sciences, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong, 999077.
Electrospray ionization (ESI)-mass spectrometry (MS) is a key platform for analyzing post-translationally modified proteins. With continuous advances in MS instruments and data analysis methods, top-down analysis of intact proteoforms has become highly feasible. To accurately quantify proteoforms with varying post-translational modifications (PTMs), the influence of PTMs on the ESI-MS detection efficiency must be considered.
View Article and Find Full Text PDFPlasma proteomic signatures accurately predict disease risk, but our understanding of the mechanisms contributing to the predictive value of the proteome remains limited. Here, we characterized proteomic biomarkers of 19 age-related diseases, based on observational associations between 2,923 protein levels and incidence of these outcomes in the UK Biobank (N = 45,438). To identify the subset of these biomarkers that may represent causal drivers of disease, we first employed Mendelian Randomization (MR) and found that only 8% of the protein-disease associations with genetic instruments showed suggestive evidence of causal relationships, and were more likely to pertain to only a single disease.
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