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Article Abstract
Here, we describe a highly efficient, medium-throughput strategy for cloning and screening of putative enhancers using the chick embryo. By incorporating 48 unique nanotags for use in NanoString nCounter® across three different fluorescent reporters and developing a rapid and efficient digestion/ligation type IIs restriction enzyme-based cloning protocol, we develop a multiplexed approach for rapidly identifying enhancer activity. For complete details on the use and execution of this protocol, please see Williams et al. (2019).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121703 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100507 | DOI Listing |
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