Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Activity-based E2 conjugating enzyme (E2)-ubiquitin (Ub) probes have recently emerged as effective tools for studying the molecular mechanism of E3 ligase (E3)-catalyzed ubiquitination. However, the preparation of existing activity-based E2-Ub probes depends on recombination technology and bioconjugation chemistry, limiting their structural diversity. Herein we describe an expedient total chemical synthesis of an E2 enzyme variant through a hydrazide-based native chemical ligation, which enabled the construction of a structurally new activity-based E2-Ub probe to covalently capture the catalytic site of Cys-dependent E3s. Chemical cross-linking coupled with mass spectrometry (CXMS) demonstrated the utility of this new probe in structural analysis of the intermediates formed during Nedd4 and Parkin-mediated transthiolation. This study exemplifies the utility of chemical protein synthesis for the development of protein probes for biological studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/anie.202105870 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Recyclable Multifunctional Ionic Liquids for Sustainable Electroorganic Oxidations.
ACS Electrochem
September 2025
School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
The study of electrochemical oxidations has wide-ranging implications, from the development of new electrocatalysts for fuel cells for energy conversion, to the synthesis of fine chemicals. 2,2,6,6-Tetramethylpiperidine-1-oxyl (TEMPO) has been used for decades as a sustainable, metal-free mediator for chemical oxidations and is now being used for electrochemical oxidations. We describe here a novel approach to TEMPO-mediated electrooxidations, in which the chemical input and waste generated during electrooxidations of alcohols are minimized by using a multifunctional room temperature ionic liquid (RTIL) to facilitate flow electrosynthesis.
View Article and Find Full Text PDFSynthesis and antibacterial properties of nanosilver-modified cellulose triacetate membranes for seawater desalination.
Beilstein J Nanotechnol
August 2025
Institute of Chemical and Industrial Bioengineering, Jilin Engineering Normal University, Changchun 130052, Jilin, People's Republic of China.
To address the issue of biological pollution in cellulose triacetate (CTA) membranes during seawater desalination, silver (Ag) nanoparticles were incorporated onto the CTA surface using polydopamine (PDA). PDA, which contains phenolic and amino groups, exhibits excellent adhesiveness and provides active sites for the attachment and reduction for Ag nanoparticles. Various characterizations confirm the successful introduction of Ag nanoparticles onto the surface of the PDA-modified CTA (PCTA) membrane and the preservation of CTA microstructures.
View Article and Find Full Text PDFPhotochemical synthesis of silver nanoprisms via green LED irradiation and evaluation of SERS activity.
Beilstein J Nanotechnol
August 2025
Nanotechnology Lab, Research Laboratories of Saigon Hi-Tech Park, Lot I3, N2 Street, Tang Nhon Phu Ward, Ho Chi Minh City 70000, Vietnam.
Silver nanoprisms (AgNPrs) are promising candidates for surface-enhanced Raman scattering (SERS) due to their strong localized surface plasmon resonance and sharp tip geometry. In this study, AgNPrs were synthesized through a photochemical method by irradiating spherical silver nanoparticle seeds with 10 W green light-emitting diodes (LEDs; 520 ± 20 nm) for various periods of time up to 72 h. The growth mechanism was investigated through ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and transmission electron microscopy analyses, confirming the gradual transformation of spherical seeds into AgNPrs.
View Article and Find Full Text PDFSolid-phase synthesis of polyunsaturated fatty acids facilitates precise structural investigation on FFAR1/4 agonist activities and the inhibition of neutrophil pseudopod formation.
RSC Adv
September 2025
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113-8656 Japan
Polyunsaturated fatty acids (PUFAs), fatty acids with multiple unsaturated carbon-carbon bonds, constitute a crucial class of lipids. While the vast diversity of PUFA species arises from their structural variations, most of them are poorly investigated due to their limited availability. Here, we utilize solid-phase synthesis of PUFAs, which we have recently developed, to construct a PUFA library.
View Article and Find Full Text PDFSelective and green conversion of 5-HMF to FDCA enzymatic (laccase) and transition metal (MnO and Co-Mn/AC) catalysis in an integrated system.
RSC Adv
September 2025
Department of Chemical Engineering and Green Technology, Institute of Chemical Technology (ICT) Mumbai Maharashtra 400019 India
The sustainable synthesis of bio-based monomers from renewable biomass intermediates is a central goal in green chemistry and biorefinery innovation. This study introduces a synergistic catalytic-enzymatic strategy for the efficient and eco-friendly oxidation of 5-hydroxymethylfurfural (5-HMF) into 2,5-furandicarboxylic acid (FDCA), a key monomer for next-generation biodegradable plastics. The catalytic phase employed non-noble metal catalysts, MnO and Co-Mn supported on activated carbon (Co-Mn/AC), under mild batch reaction conditions at 90 °C.
View Article and Find Full Text PDF