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Article Abstract
In this work, we develop a direct transverse relaxation time () biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol-gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a change of water molecules. By means of enzyme-modulated sol-gel transition and antigen-antibody interactions, this biosensor displays high sensitivity for detecting 50 CFU/mL within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection.
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| http://dx.doi.org/10.1021/acs.analchem.0c03968 | DOI Listing |
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