Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for identification of transgenic roots. The MtLAP1 transcription factor has been utilized as a reporter system in based on production of anthocyanin pigment. Here, we describe a version of this reporter driven by a root-cap specific promoter for direct observation of anthocyanin accumulation in root tips, which allows the identification of transgenic hairy roots by the naked eye. Results from our analysis suggest that the reporter had no significant effects on nodulation of . This approach, by virtue of its strong and specific expression in root cap cells, greatly reduces false positives and false negatives, and its use of an easily scored visible pigment should allow greater versatility and efficiency in root biology studies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004629 | PMC |
| http://dx.doi.org/10.3390/plants10030605 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of GABBR2 as a diagnostic marker and its association with Aβ in Alzheimer's disease.
Biochem Biophys Rep
June 2025
Department of Public Health, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Background: Synaptic dysfunction and synapse loss occur in Alzheimer's disease (AD). The current study aimed to identify synaptic-related genes with diagnostic potential for AD.
Methods: Differentially expressed genes (DEGs) were overlapped with phenotype-associated module selected through weighted gene co-expression network analysis (WGCNA), and synaptic-related genes.
Intestinal epithelial Dicer1 regulates gut microbiome and Alzheimer's pathology in App-knock-in mice.
Alzheimers Res Ther
September 2025
Department of Neurology, Saarland University, Kirrberger Straße, 66421, Homburg/Saar, Germany.
Background: Alzheimer's disease (AD) patients and animal models exhibit an altered gut microbiome that is associated with pathological changes in the brain. Intestinal miRNA enters bacteria and regulates bacterial metabolism and proliferation. This study aimed to investigate whether the manipulation of miRNA could alter the gut microbiome and AD pathologies.
View Article and Find Full Text PDFIdentification of a carotenoid cleavage dioxygenase gene TeCCD4a regulating flower color and carotenoid content of marigold.
Gene
September 2025
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
Marigold (Tagetes erecta) serves as both an ornamental and economically significant species, owing to its diverse floral coloration and exceptionally high petal carotenoid content. Carotenoid cleavage dioxygenase (CCD), as the key enzymatic component, mediates the carotenoid degradation process. In this study, we cloned and functionally characterized a CCD4 gene to elucidate its regulatory function in petal color and carotenoid biosynthesis.
View Article and Find Full Text PDFA GFP Complementation-based Dual-expression System for Assessing Cell-Cell Contact Mediated by Cytonemes in Live Drosophila Wing Imaginal Discs.
J Vis Exp
August 2025
Institut de recherches cliniques de Montréal (IRCM); Programmes de biologie moléculaire, Université de Montréal; Département de Médecine, Université de Montréal;
Embryonic tissue growth and patterning are largely controlled by signals exchanged locally between cell populations within the tissues themselves. Cytonemes are a type of signaling filopodia first identified in Drosophila that connect and mediate exchange between signal-producing and signal-receiving cells. In the developing Drosophila wing imaginal disc, cytonemes are involved in signal exchange between distinct populations of cells within the disc proper (DP) epithelium, which will form the adult wing, as well as between DP cells and cells in adjacent disc-associated tissues.
View Article and Find Full Text PDFGenome-wide identification of xyloglucan endotransglucosylase/hydrolase genes in almond and PdXTH2 overexpression induces cell structural disassembly and promotes programmed cell death progression.
Plant Biol (Stuttg)
September 2025
College of Horticulture, Xinjiang Agricultural University, Urumqi, China.
The xyloglucan endotransglucosylase/hydrolase (XTH) gene family plays a crucial role in fruit development and texture regulation. We used the telomere-to-telomere genome of 'Wanfeng' almond to identify and analyse the XTH gene family, examining protein physicochemical properties, phylogenetic relationships, protein conserved motifs, gene structure, gene replication, and expression patterns. We identified 23 PdXTH family members through the telomere-to-telomere level genome of 'Wanfeng' almond.
View Article and Find Full Text PDF