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This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated W1 (EPS-W1), which belongs to genus , is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopolysaccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of →6)-d-Glcp-(1→; →3)-d-Manp-(1→; →3)-d-Glcp-(1→ and a branch of →6)-d-Manp-(1→; →2)-d-Glcp-(1→. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications.
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http://dx.doi.org/10.1021/acsomega.0c05256 | DOI Listing |
Bioelectrochemistry
September 2025
Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, 11371 Cairo, Egypt. Electronic address:
The rapid increase in population has driven the demand for fossil fuel energy, contributing to increased carbon emissions that ultimately accelerate global warming and climate change. Battery storage systems have many advantages over conventional energy sources. However, they face limitations such as energy storage, cost, and environmental hazards that come with the use of chemical binders.
View Article and Find Full Text PDFBiotechnol Rep (Amst)
September 2025
Technical University of Munich, Germany, TUM Campus Straubing for Biotechnology and Sustainability, Bioprocess Engineering, Uferstraße 53, D-94315 Straubing, Germany.
Exopolysaccharides are biopolymers with wide-ranging industrial applications. To substitute fossil-based by bio-based, biodegradable polymers, exopolysaccharide production needs to become much more efficient. Pullulan, produced by , is popular for its unique properties like film-formation, adhesiveness, biodegradability, etc.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life
The low yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) restricts their industrial application. To overcome this limitation, a single-factor optimization strategy was applied to develop co-culture system involving Weissella confusa P2 and Candida shehatae. This approach resulted in 48.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
September 2025
Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
Glycoside hydrolase family 27 (GH27) consists primarily of exo-acting enzymes, including α-galactosidase, α-N-acetylgalactosaminidase, β-L-arabinopyranosidase, and isomalto-dextranase. Here, we examined the gene expression profiles and biochemical characteristics of a multi-domain protein, Fjoh_4436 (named FjGH27A), which contains a GH27 catalytic domain and is encoded upstream of the previously described branched dextran utilization locus in Flavobacterium johnsoniae. Expression of this gene increased 7.
View Article and Find Full Text PDFBiomed Pharmacother
September 2025
APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland; Cancer Research @UCC, College of Medicine and Health, University College Cork, T12 K8AF Cork, Ireland.
Microbial exopolysaccharides (EPS) are emerging as critical effector molecules orchestrating host immunity. Their remarkable structural diversity, driven by variations in monosaccharide composition, charge, and glycosidic linkages, dictates potent immunomodulatory functions through specific interactions with host pattern recognition receptors (CLRs, TLRs), triggering defined cellular responses and cytokine profiles, often with minimal toxicity. Beyond direct immune effects, EPS shape the gut environment by fortifying barrier integrity and acting as prebiotics.
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