Carnimonas bestiolae sp. nov. and Cernens ardua gen. nov., sp. nov.: new halotolerant bacteria from the invasive solitary bee Megachile sculpturalis.

Fifteen isolates from gut samples of the invasive solitary bee Megachile sculpturalis remained unidentified after matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. Phylogenomic and overall genome relatedness indices analyses of five representative isolates demonstrated that 14 isolates represented a novel Carnimonas species for which we propose the name Carnimonas bestiolae sp. nov.

Carotenoid biosynthesis in bacteria: the gene products and their functional roles in the carotenogenic pathways.

Carotenoids are isoprenoid pigments that are largely responsible for the red, pink, orange, and yellow pigmentation in bacteria. Despite their structural diversity, they share a similar general chemical structure. Carotenogenesis is a complex, multistep process, mediated by the gene products.

Judicial Opinion 132.

Opinion 132 deals with a Request for an Opinion asking the Judicial Commission to assess the nomenclatural status of (Blackall 1989) Chun 1997, a new combination affected by an unusual change in the epithet of its basonym, Blackall 1989. This is done by re-evaluating Opinion 85, which sets a precedent. The reasons and consequences of this earlier Opinion are explained in detail, and corresponding guidelines for orthographic corrections are given, along with further guidance derived from more recently published Judicial Opinions.

Unravelling Listeria monocytogenes persistence in frozen potato processing: Insights from whole genome sequencing analysis.

Listeria monocytogenes persistence remains an issue in the food industry, including the frozen vegetable/potato processing industry. This study demonstrates the added value of whole genome sequencing (WGS) for persistence and transmission routes within food businesses. A total of 79 isolates from environmental samples (including employees' shoes), collected over three years (2020-2022), along with two intermediate product samples, and one end product sample, and submitted for WGS.

Identification and functional genomic analyses of Bartonella isolates from honey bees, and reassessment of the taxonomy of the genus Bartonella.

We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole-genome sequence analyses to identify 90 Bartonella isolates from honey bee gut samples in Belgium. While the identification of 62 isolates as Bartonella apihabitans and three as Bartonella choladocola was straightforward, the identification of 25 Bartonella apis-like isolates was challenging. A taxonomic and functional analysis of four B.

The unresolved struggle of 16S rRNA amplicon sequencing: a benchmarking analysis of clustering and denoising methods.

Background: Although 16S rRNA gene amplicon sequencing has become an indispensable method for microbiome studies, this analysis is not error-free, and remains prone to several biases and errors. Numerous algorithms have been developed to eliminate these errors and consolidate the output into distance-based Operational Taxonomic Units (OTUs) or denoising-based Amplicon Sequence Variants (ASVs). An objective comparison between them has been obscured by various experimental setups and parameters.

gen. nov., sp. nov., a facultatively autotrophic bacterium from environmental water samples.

A Gram-stain-negative coccobacillus, LMG 32992, was isolated from water that had collected in a tyre in Pingtung, Donggang Township, Taiwan. Upon preliminary 16S rRNA gene sequence analysis, it was most closely related to members of the genus (16S rRNA gene sequence similarities of 96.7-97.

Judicial Opinion 131.

Opinion 131 addresses a Request for an Opinion asking the Judicial Commission to conserve the genus name Hauser 1885 (Approved Lists 1980) over its earlier homonym, the protozoan genus name Müller 1786. The Judicial Commission agrees that the later homonym is illegitimate and that the replacement of the prokaryotic name would be undesirable. It is also concluded that Müller 1786 is an objectively invalid name under the .

Pre-trained Maldi Transformers improve MALDI-TOF MS-based prediction.

For the last decade, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been the reference method for species identification in clinical microbiology. Hampered by a historical lack of open data, machine learning research towards models specifically adapted to MALDI-TOF MS remains in its infancy. Given the growing complexity of available datasets (such as large-scale antimicrobial resistance prediction), a need for models that (1) are specifically designed for MALDI-TOF MS data, and (2) have high representational capacity, presents itself.

Microbial ecology and metabolite dynamics of backslopped triticale sourdough productions and the impact of scale.

Triticale (X Triticosecale Wittmack) is a hybrid of wheat (Triticum aestivum L.) and rye (Secale cereale L.), combining the positive attributes of both cereals.

Benchmarking short-, long- and hybrid-read assemblers for metagenome sequencing of complex microbial communities.

Metagenome community analyses, driven by the continued development in sequencing technology, is rapidly providing insights in many aspects of microbiology and becoming a cornerstone tool. Illumina, Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio) are the leading technologies, each with their own advantages and drawbacks. Illumina provides accurate reads at a low cost, but their length is too short to close bacterial genomes.

Judicial Opinion 130.

Opinion 130 deals with a Request for an Opinion asking the Judicial Commission to clarify whether the genus name Zopf 1891 (Approved Lists 1980) is illegitimate. The Request is approved and an answer is given. The name Zopf 1891 (Approved Lists 1980) is illegitimate because it is a later homonym of the validly published cyanobacterial name Hansgirg 1884.

gen. nov., sp. nov., a novel fructophilic lactic acid bacterium from bumble bees.

Strain LMG 33000 was isolated from a gut sample. It shared the highest percentage 16S rRNA sequence identity, average amino acid identity, and amino acid identity of conserved genes with LMG 28291 (95.86 %, 69.

A comparative genomic analysis of Fructobacillus evanidus sp. nov. from bumble bees.

The increase in studies on bee microbiomes is prompted by concerns over global pollinator declines. Bumble bees host core and non-core microbiota which may contribute to increased lifetime fitness. The presence of Fructobacillus in the gut microbiomes of bumble bee workers, or the replacement of core symbionts with Fructobacillus bacteria, has been considered a marker of dysbiosis.

Refermentation and maturation of lambic beer in bottles: a necessary step for gueuze production.

The production of gueuze beers through refermentation and maturation of blends of lambic beer in bottles is a way for lambic brewers to cope with the variability among different lambic beer batches. The resulting gueuze beers are more carbonated than lambic beers and are supposed to possess a unique flavor profile that varies over time. To map this refermentation and maturation process for gueuze production, a blend of lambic beers was made and bottled, whereby one of them was produced with the old wheat landrace .

Proposal of three novel insect-associated species: sp. nov., sp. nov. and sp. nov.

A recent modification of the Note to Rule 25a of the International Code for Nomenclature of Bacteria is used by the List Editors of the to justify the refusal to validate species protologues published in supplementary material to this formal decision. Authors are therefore forced to ask permission to reuse published data for the valid publication of such names. In the present letter we re-publish the species protologues of sp.

The best of both worlds: a proposal for further integration of names into the International Code of Nomenclature of Prokaryotes.

The naming of prokaryotes is governed by the International Code of Nomenclature of Prokaryotes (ICNP) and partially by the International Code of Nomenclature for Algae, Fungi and Plants (ICN). Such codes must be able to determine names of taxa in a universal and unambiguous manner, thus serving as a common language across different fields and activities. This unity is undermined when a new code of nomenclature emerges that overlaps in scope with an established, time-tested code and uses the same format of names but assigns different nomenclatural status values to the names.

Various cold storage-backslopping cycles show the robustness of Limosilactobacillus fermentum IMDO 130101 as starter culture for Type 3 sourdough production.

Type 3 sourdoughs, which are starter culture-initiated and subsequently backslopped, are less studied than other sourdough types. Yet, they can serve as a model to assess how competitive starter culture strains for sourdough production are and how the microbial composition of such sourdoughs may evolve over time. In the present study, Limosilactobacillus fermentum IMDO 130101 was used to produce Type 3 sourdoughs, prepared from wheat and wholemeal wheat flours.

The wild solitary bees Andrena vaga, Anthophora plumipes, Colletes cunicularius, and Osmia cornuta microbiota are host specific and dominated by endosymbionts and environmental microorganisms.

We characterized the microbial communities of the crop, midgut, hindgut, and ovaries of the wild solitary bees Andrena vaga, Anthophora plumipes, Colletes cunicularius, and Osmia cornuta through 16S rRNA gene and ITS2 amplicon sequencing and a large-scale isolation campaign. The bacterial communities of these bees were dominated by endosymbionts of the genera Wolbachia and Spiroplasma. Bacterial and yeast genera representing the remaining predominant taxa were linked to an environmental origin.

Comparative genomic analysis of and the characterization of novel motile species.

The genus was proposed as a novel genus in the in 2022. However, the phylogenetic relationship between and other heterofermentative lactobacilli, and the genetic and physiological properties of this genus remain unclear. This study aimed to determine the phylogenetic relationship between and the two closest genera, and , by the phylogenetic analysis and calculation of (core gene) pairwise average amino acid identity.