The Regulatory Network of in Wheat- f. sp. Interaction Revealed by Temporal Profiling Using RNA-Seq.

Wheat powdery mildew (Pm), caused by f. sp. (), is a prevalent fungal disease. The diploid wheat relative () showed broad-spectrum resistance (BSR) to Pm. A previous study reported an E3 ligase gene, from , showing BSR to Pm. To elucidate the regulatory network mediated by , in this study, gene expression profiling of transgenic plant () and its receptor Yangmai 158 was analyzed and compared after inoculation at four infection stages. GO and KEGG analysis revealed obvious reprogramming of SA and ABA signaling, starch/sucrose metabolism, and photosynthesis in , compared with those in Yangmai 158. Transcripts of SA synthesis genes and , signaling factors and , and in ABA signaling were specifically upregulated in rather than Yangmai 158. Transcripts of in photosynthesis, and in starch/sucrose metabolism were also induced distinctly in . WGCNA analysis showed crucial regulatory candidates of , involving serine/threonine-protein kinase in phosphorylation, glucosyltransferase in flavonoid biosynthesis, defense factor WRKYs, and peroxidase in oxidative stress. Our results facilitate the deciphering of the resistant regulatory network of and the identification of key candidates which might be employed in breeding programs.