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A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from . The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test. Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential. The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 10 kDa and a specific optical rotation of +155°. The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose. Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.
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http://dx.doi.org/10.1080/14786419.2020.1795657 | DOI Listing |
Nucleic Acids Res
September 2025
School of Microbiology, University College Cork, Cork, T12 Y337, Ireland.
The genomes of 43 distinct lactococcal strains were reconstructed by a combination of long- and short-read sequencing, resolving the plasmid complement and methylome of these strains. The genomes comprised 43 chromosomes of approximately 2.5 Mb each and 269 plasmids ranging from 2 to 211 kb (at an average occurrence of 6 per strain).
View Article and Find Full Text PDFMicrobiologyopen
October 2025
Department of Agronomy, National Taiwan University, Taipei, Taiwan.
Currently, there is an increasing use of whole-genome sequencing (WGS) studies to investigate the molecular taxonomy, metabolic properties, enzyme capabilities, and bioactive substances of lactic acid bacteria (LAB) species. In this study, the genome of strain Pediococcus pentosaceus BBS1 was sequenced using the Illumina HiSeq. 2500 platform to determine its classification, annotate its main features, and evaluate its safety characteristics.
View Article and Find Full Text PDFKnee Surg Sports Traumatol Arthrosc
September 2025
Department of Orthopaedics and Trauma Surgery, Medical University of Innsbruck, Innsbruck, Austria.
Purpose: Modifying interference screw composition may ensure better osteoconductive properties in order to reduce tunnel enlargement after anterior cruciate ligament (ACL) reconstruction. The primary and secondary purposes were to evaluate tunnel and screw volume changes in poly-L-lactide acid (PLLA) and poly-D-lactic acid + hydroxyapatite + β-tricalcium phosphate (PLDLA+) screws. The tertiary purpose was to compare patient reported- and functional outcomes between PLLA and PLDLA+ group.
View Article and Find Full Text PDFEur J Sport Sci
October 2025
Department of Intervention Research in Exercise Training, German Sport University Cologne, Cologne, Germany.
The concurrent validity of lactate thresholds (LT1, LT2) and between-day reliability data from the rowing-specific heart rate variability (HRV)-based thresholds (HRVT) were examined. Thus, 21 rowers (19.6 ± 2.
View Article and Find Full Text PDFMedicine (Baltimore)
September 2025
Department of Infectious Diseases, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Dichloroacetate (DCA), as a pan-inhibitor of pyruvate dehydrogenase kinase, plays a crucial role in energy metabolism and mitochondrial function. DCA decreases lactic acid synthesis, enhances mitochondrial oxidative phosphorylation, and regulates aerobic glycolysis. During the last decade, more and more studies have found that disorders of energy metabolism and mitochondrial dysfunction play a pivotal role in the development and progression of various diseases, and the role of DCA in cancer, metabolic diseases, and inflammatory diseases has been extensively explored in both basic and clinical studies.
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