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In the last two decades, food scientists have attempted to develop new technologies that can improve the detection of insect infestation in fruits and vegetables under postharvest conditions using a multitude of non-destructive technologies. While consumers' expectations for higher nutritive and sensorial value of fresh produce has increased over time, they have also become more critical on using insecticides or synthetic chemicals to preserve food quality from insects' attacks or enhance the quality attributes of minimally processed fresh produce. In addition, the increasingly stringent quarantine measures by regulatory agencies for commercial import-export of fresh produce needs more reliable technologies for quickly detecting insect infestation in fruits and vegetables before their commercialization. For these reasons, the food industry investigates alternative and non-destructive means to improve food quality. Several studies have been conducted on the development of rapid, accurate, and reliable insect infestation monitoring systems to replace invasive and subjective methods that are often inefficient. There are still major limitations to the effective in-field, as well as postharvest on-line, monitoring applications. This review presents a general overview of current non-destructive techniques for the detection of insect damage in fruits and vegetables and discusses basic principles and applications. The paper also elaborates on the specific post-harvest fruit infestation detection methods, which include principles, protocols, specific application examples, merits, and limitations. The methods reviewed include those based on spectroscopy, imaging, acoustic sensing, and chemical interactions, with greater emphasis on the noninvasive methods. This review also discusses the current research gaps as well as the future research directions for non-destructive methods' application in the detection and classification of insect infestation in fruits and vegetables.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404779 | PMC |
http://dx.doi.org/10.3390/foods9070927 | DOI Listing |
J Econ Entomol
September 2025
European Biological Control Laboratory (EBCL USDA ARS), Montferrier-sur-lez, France.
Evaluating the olfactory preferences of emerging insect pests is critical to develop monitoring tools and improve early detection and management strategies. Here the chemical ecology and olfactory preferences of the allium leafminer Phytomyza gymnostoma Loew (Diptera: Agromyzidae), an invasive pest in North America affecting allium crops such as leeks and onions, were investigated. Three bioassay methods were assessed under laboratory conditions: wind tunnel, Y-tube olfactometer, and arena bioassay.
View Article and Find Full Text PDFParasite
September 2025
Parasitology Department, São Paulo University, 1374 Av. Prof. Lineu Prestes, São Paulo, State of São Paulo 05508-000, Brazil.
Understanding why Diptera, such as mosquitoes and sand flies, feed on humans is crucial in defining them as vectors of diseases such as malaria, dengue fever, Zika virus, and leishmaniasis. Determining their attraction to humans (anthropophily) helps in assessing the risk of disease transmission, designing effective vector control strategies, and monitoring the effectiveness of existing control measures. An important question is whether they are specifically attracted to humans in preference to other mammals or whether there is something else at play.
View Article and Find Full Text PDFApidologie
September 2025
Institute of Bee Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Unlabelled: High infestation levels of small hive beetle (SHB), , can cause more damage to honeybee, , host colonies. However, the spatiotemporal variation of SHB infestations is poorly understood. Here, we show that SHB infestations can be equally high in native and invasive ranges, suggesting that differences between host populations are the key criterion for damage.
View Article and Find Full Text PDFPestic Biochem Physiol
November 2025
Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100
The insect midgut peritrophic membrane (PM) plays important roles in insect-microbe interactions. Bacillus thuringiensis (Bt) and its proteinaceous toxins are widely used for insect control. To understand the role of PM in insects against Bt toxins, this study selected Grapholita molesta Busck (Lepidoptera: Tortricidae), a worldwide pest infesting fruit trees, as the research subject.
View Article and Find Full Text PDFPestic Biochem Physiol
November 2025
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, Jiangsu, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Inst
The molecular mechanism of baculovirus infection is the basis of baculovirus wide application. Identifying and elucidating the functional genes of virus replication is the focus of research. Eukaryotic initiation factor 4E (eIF4E) is a key component of the translation initiation process to synthesize proteins required for replication.
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