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Background: Marker gene surveys have a wide variety of applications in species identification, population genetics, and molecular epidemiology. As these methods expand to new types of organisms and additional markers beyond 16S and 18S rRNA genes, comprehensive databases are a critical requirement for proper analysis of these data.
Results: Here we present an ITS2 rDNA database for marker gene surveys of both free-living and parasitic nematode populations and the software used to build the database. This is currently the most complete and up-to-date ITS2 database for nematodes and is able to reproduce previous analysis that used a smaller database.
Conclusions: This database is an important resource for researchers working on nematodes and also provides a tool to create ITS2 databases for any given taxonomy.
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http://dx.doi.org/10.1186/s12863-020-00880-0 | DOI Listing |
ACS Biomater Sci Eng
September 2025
Materials Engineering, McGill university, Montreal H3A0C5, Canada.
Transcutaneous devices such as dental implants frequently fail due to infections at their interfaces with epithelial tissues. These infections are facilitated by the lack of integration between the devices and the surrounding soft tissues. This study aims to improve epithelial integration through surface modification of a transcutaneous implant material (polyetheretherketone (PEEK)).
View Article and Find Full Text PDFAnn Bot
September 2025
Royal Botanic Gardens, Kew, Richmond, Research department, Surrey, TW9 3AE, UK.
Background And Aims: Crop wild relatives (CWRs) are key resources for enhancing agricultural resilience, providing genetic traits that can improve pest resistance, abiotic stress tolerance, and nutritional composition in domesticated crops. Within the mustard family (Brassicaceae) this is especially significant in the Brassiceae tribe, which includes economically important genera for agriculture such as Brassica and Sinapis. However, while breeding programmes have historically focused on major crops within this tribe, the potential of their wild relatives, particularly for underutilised and minor crops, remains insufficiently explored.
View Article and Find Full Text PDFAm J Reprod Immunol
September 2025
Department of Laboratory Animal Science, Kunming Medical University, Kunming, China.
Objective: To explore B cell infiltration-related genes in endometriosis (EM) and investigate their potential as diagnostic biomarkers.
Methods: Gene expression data from the GSE51981 dataset, containing 77 endometriosis and 34 control samples, were analyzed to detect differentially expressed genes (DEGs). The xCell algorithm was applied to estimate the infiltration levels of 64 immune and stromal cell types, focusing on B cells and naive B cells.
PLoS Negl Trop Dis
September 2025
Department of Clinical Science, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
Background: Salmonella enterica encompasses over 2,600 serovars, including several commonly associated with severe infection in humans. Salmonella is a major cause of sepsis in Africa; however, diagnosis requires clinical microbiology facilities. Environmental surveillance has the potential to play a role in Salmonella surveillance.
View Article and Find Full Text PDFPLoS One
September 2025
Biobank of Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China.
Heart failure (HF) and lung cancer (LC) often coexist, yet their shared molecular mechanisms are unclear. We analyzed transcriptome data from the NCBI Gene Expression Omnibus (GEO) database (GSE141910, GSE57338) to identify 346 HF‑related differentially expressed genes (DEGs), then combined weighted gene co-expression network analysis (WGCNA) pinpointed 70 hub candidates. Further screening of these 70 hub candidates in TCGA lung cancer cohorts via LASSO, Random Forest, and multivariate Cox regression suggested CYP4B1 as the only independent prognostic marker.
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