Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Surgical resection is widely used for tumor treatment, necessitating approaches for the precise locating of elusive tumor foci. We report the high performance detection of tumors in mice with fluorescein-isothiocyanate (FITC) labelled sialic acid (FITC-SA), a fluorescent monosaccharide with low cytoxicity. Analysis of mice intravenously injected with FITC-SA revealed high target-to-background fluorescence ratios in subcutaneous tumors and liver tumor implants with 0.2-5 mm diameters, which are significantly below the clinical threshold of minimal residual cancer (∼1 cm clearance). Extracellular FITC-SA is quickly cleared from circulation whereas the intracellular FITC-SA could be metabolically incorporated into glycoproteins via a cellular sialylation pathway. Compared with FITC-SA-laden nanoparticles, free FITC-SA is preferentially and quickly taken up by tumors in mice and displays high tumor-to-background signal contrast, suggesting the potential for fluorescence directed surgical ablation of tumors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c4bm00028e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication of phenylboronic acid modified graphene oxide-silica biosensor for label-free electrochemical detection of circulating tumor cells.
Bioelectrochemistry
September 2025
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan. Electronic address:
Early and accurate detection of circulating tumor cells (CTCs) is vital for cancer diagnosis and personalized treatment. Despite their clinical significance, the identification of CTCs remains challenging because of the biological complexity and lower concentration. Therefore, a cost-effective, and label-free electrochemical biosensor based on phenyl boronic acid functionalized graphene oxide-silica (PBA@GO-SiO) is developed to selectively recognize sialic acid-rich glycoproteins on HepG2 cells.
View Article and Find Full Text PDFSialoglycoconjugate profiling of human choroid, retinal pigment epithelium, and basal laminar deposits.
Exp Eye Res
September 2025
Institute for Vision Research, The University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA. Electronic address:
Age-related macular degeneration is a leading cause of central vision loss in the elderly. Early hallmarks of the disease include basal laminar deposit beneath the retinal pigment epithelium (RPE) and choriocapillaris degeneration. We utilized sialic acid binding lectins Sambucus nigra/Elderberry Bark Lectin (EBL) and Maackia amurensis lectin II (MAL-II), to assess the localization of ɑ-2,6 and ɑ-2,3 sialic acids, respectively, in human macular retina, RPE, basal laminar deposits, and choroid.
View Article and Find Full Text PDFLabel-Free HPLC Method to Evaluate Neuraminidase Activity in Biological Fluids Containing the Newcastle Disease Virus.
Biomed Chromatogr
October 2025
Malaria Research Group, Department of Biosciences and Bioengineering, Indian Institute of Technology-Guwahati, Guwahati, Assam, India.
Newcastle disease virus utilizes its multifunction hemagglutinin neuraminidase (HN) protein for sialic acid recognition and its cleavage from the adjacent lactose unit. Detection of neuraminidase activity of HN is crucial for studying NDV infection biology. Traditional neuraminidase assays predominantly employ synthetic fluorogenic substrates such as 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid.
View Article and Find Full Text PDFChemical engineering of γδ T cells with cancer cell-targeting antibodies for enhanced tumor immunotherapy.
Natl Sci Rev
August 2025
Department of Pharmacy, Peking University Third Hospital Cancer Center, Peking University Third Hospital, Beijing 100191, China.
Gamma delta (γδ) T cells hold great promise in adoptive cell therapy, but suffer from low tumor-targeting efficiency. Herein, we report the development of antibody-γδ T cell conjugates for enhanced tumor therapy. By evaluating different biomolecules residing on the cell surface, sialic acids-the terminal sugars of various cell-surface glycans-are identified as the optimum site for anchoring antibodies onto γδ T cells via metabolic glycan labeling with unnatural sugars containing a bioorthogonal functional group.
View Article and Find Full Text PDFFast Screening of Circulating Tumor Cells in Blood Samples by Sialic Acid Recognition and Microfluidic Chip-Single-Cell ICP-MS: A Breakthrough in Non-invasive Cancer Diagnosis.
Anal Chem
September 2025
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Circulating tumor cells (CTCs) hold promise for non-invasive cancer diagnostics, but current detection methods face challenges like white blood cell (WBC) interference, high false negative rates, low throughput, and operational complexity. To overcome these, we developed a simple, fast, and universal CTCs analysis method by the integrating sialic acid (SA) recognition strategy, microfluidic cell sorting, and online single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) detection. Cells from lysed blood sample were labeled by phenylboronic acid-functionalized gold nanoparticles (PBA-AuNPs) via PBA-SA recognition.
View Article and Find Full Text PDF