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Article Abstract
The relationship between calcium ion (Ca) concentration and temperature variation during the oxidative phosphorylation (OXPHOS) process has become an essential focus for exploration of signaling pathways and neurodegenerative disease. However, there have been limited reports of fluorescent probes for simultaneous Ca detection and temperature sensing. Herein, a new water-soluble fluorescent probe that combines a thermoresponsive polymer, curcumin and Fluo-4 AM for intracelllar temperature and Ca sensing is described. Furthermore, this fluorescent polymer was successfully applied for intracelluar temperature and Ca gradient monitoring generated by exogenous heating in HeLa cells. It was discovered that within 10 min after the OXPHOS process was induced by an inhibitor, the temperature increased 0.5-1.0 °C and the Ca level decreased by about 5.7 μM. These results confirmed that the fluorescent polymer enabled investigation of the relationship between intracelluar temperature and Ca-induced neurotransmitter release.
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| http://dx.doi.org/10.1021/acs.analchem.0c01534 | DOI Listing |
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