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Article Abstract
Statistical experimental designs were used to optimize conditions for recombinant feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal conditions for BpFae production were: 6 g/L lactose, pH 5.5, pre-induced period 5 h, 23 °C, shaker rotational speed of 240 rpm, medium volume of 50 mL/250 mL, inoculum size 0.2% (v/v), and a post-induced period of 32 h in a Luria-Bertani culture. The produced BpFae activity was 7.43 U/mL, which is 2.92 times higher than that obtained under optimal conditions using IPTG as the inducer. BpFae activity was 4.82 U/mL in a 5 L fermenter under the abovementioned optimal conditions. BpFae produced a small amount of ethyl acetate but had no effect on the synthesis of other important esters in . The results underpin further investigations into BpFae characterization and potential applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186284 | PMC |
| http://dx.doi.org/10.1007/s13205-020-02198-1 | DOI Listing |
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