Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Rapid detection of cysteine oxidation in living cells is critical in advancing our understanding of responses to reactive oxygen species (ROS) and oxidative stress. Accordingly, there is a need to develop chemical probes that facilitate proteome-wide detection of cysteine's many oxidation states. Herein, we report the first whole-cell proteomics analysis using a norbornene probe to detect the initial product of cysteine oxidation: cysteine sulfenic acid. The oxidised proteins identified in the HeLa cell model represent the first targets of the ROS hydrogen peroxide. The panel of protein hits provides new and important information about the targets of oxidative stress, including 148 new protein members of the sulfenome. These findings provide new leads for the study and understanding of redox signalling and diseases associated with oxidative stress.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cbic.201900729 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
N-acetyl cysteine for the treatment of alcohol use disorder: study protocol for a multi-site, double-blind randomised controlled trial (NAC-AUD study).
BMJ Open
September 2025
Specialty of Addiction Medicine, The University of Sydney School of Medicine, Sydney, New South Wales, Australia.
Introduction: Current treatments for alcohol use disorders (AUD) have limited efficacy. A previous 28-day pilot trial of N-acetyl cysteine (NAC) vs placebo found NAC to be feasible and safe, with evidence of improvement on some measures of alcohol consumption. Thus, the primary aim of the NAC-AUD study is to examine the therapeutic and cost-effectiveness of NAC vs placebo in improving treatment outcomes for AUD.
View Article and Find Full Text PDFDifferential ligation alters electronic state and coupling signals of iron-sulfur clusters in flavin-based electron bifurcation.
J Inorg Biochem
September 2025
National Renewable Energy Laboratory, Biosciences Center, Golden, CO, USA. Electronic address:
Flavin-based electron bifurcation (FBEB) is employed by microorganisms for controlling pools of redox equivalents by reversibly splitting electron pairs into high- and low-energy levels from an initial midpoint potential. Our ability to harness this phenomenon is crucial for biocatalytic design which is limited by our understanding of energy coupling in the bifurcation system. In Pyrococcus furiosus, FBEB is carried out by the NADH-dependent ferredoxin:NADP-oxidoreductase (NfnSL), coupling the uphill reduction of ferredoxin in NfnL to the downhill reduction of NAD in NfnS from oxidation of NADPH.
View Article and Find Full Text PDFFABP4-dependent fatty acid oxidation-fueled mitochondrial ROS induces the mobilization of cellular iron and facilitates proliferation in murine adipocytes.
mBio
September 2025
Department of Microbiology & Immunology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
Fatty acid-binding protein 4 (FABP4) is a cytosolic lipid chaperone predominantly expressed in adipocytes. It has been shown that targets adipose tissues and resides in adipocytes. However, how manipulates adipocytes to redirect nutrients for its benefit remains unknown.
View Article and Find Full Text PDFSparc Suppresses Microglial Neuroinflammation and Promotes Axonal Regeneration by Interacting With Uba52.
Front Biosci (Landmark Ed)
August 2025
Department of Spine Surgery, Zhongda Hospital Southeast University, 210009 Nanjing, Jiangsu, China.
Background: After spinal cord injury (SCI), pro-inflammatory microglia accumulate and impede axonal regeneration. We explored whether secreted protein acidic and rich in cysteine (Sparc) restrains microglial inflammation and fosters neurite outgrowth.
Methods: Mouse microglial BV2 cells were polarized to a pro-inflammatory phenotype with lipopolysaccharides (LPSs).
Activity-Based Ubiquitin Probes Capture the Sulfenylated State of Deubiquitinases.
Angew Chem Int Ed Engl
September 2025
State Key Laboratory of Chemo and Biosensing, School of Biomedical Sciences, Hunan University, Changsha, 410082, China.
Activity-based ubiquitin probes (Ub-ABPs) are powerful tools for studying the functional landscape of deubiquitinases (DUBs). While most existing Ub probes have focused on examining the native state of DUBs, oxidative stress, especially in cancer and inflammatory contexts, can oxidize the catalytic cysteine of DUBs, significantly altering their activity. Here, we developed three novel ubiquitin-based activity probes (Ub-ABPs) to selectively trap the sulfenylated form of deubiquitinases (DUB-SOH).
View Article and Find Full Text PDF