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Complete genome sequences of two cycas necrotic stunt virus (CNSV) isolates from Paeonia suffruticosa and Daphne odora were determined. Phylogenetic trees and pairwise comparisons using complete RNA1- and RNA2-encoded polyproteins showed that the two CNSV isolates are divergent (83.19%-89.42% in polyprotein 1 and 73.61%-85.78% in polyprotein 2). A comparative analysis based on taxonomic criteria for the species demarcation of nepoviruses confirmed that they are not new species but distinct variants. This is the first report of the complete genome sequences of CNSV detected in P. suffruticosa and D. odora, and the first report of CNSV infecting P. suffruticosa.
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http://dx.doi.org/10.1007/s11262-019-01687-7 | DOI Listing |
Sci Rep
May 2025
Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Toxoplasmosis treatments are currently limited due to their ineffectiveness against the chronic stage and potential adverse effects. The search for novel medications that possess characteristics such as non-toxicity, and efficacy on all stages of the parasite, particularly the cystic form, is important. The n-butanol (n-BuOH) fraction of Cycas rumphii has shown promising potency against Toxoplasma gondii (RH strain) in vitro, and this study aimed to evaluate its efficacy against T.
View Article and Find Full Text PDFViruses
May 2024
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Increasing reports of tobacco rattle virus (TRV) and cycas necrotic stunt virus (CNSV) in herbaceous worldwide highlight the importance of conserving the genetic resources of this economically important ornamental and medicinal crop. The unknown origin(s) of infection, differential susceptibility of peony cultivars to these viruses, and elusive disease phenotypes for CNSV in peonies make early detection and management challenging. Here, we report the presence of TRV and CNSV in plants of the University of Michigan living peony collection in the United States and a molecular characterization of their strains.
View Article and Find Full Text PDFAntibiotics (Basel)
July 2022
Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt.
Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate the effect of the secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on Enterococcus faecalis (E.
View Article and Find Full Text PDFVirus Res
February 2022
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, United States. Electronic address:
Cycas necrotic stunt virus (CNSV) has an extensive host range and is detected in an accelerated pace around the globe in several agricultural crops. One of the plant species affected is peony (Paeonia lactiflora Pall.).
View Article and Find Full Text PDFPlant Dis
December 2021
College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China.
To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus, apple stem grooving virus, lychnis mottle virus, grapevine line pattern virus, and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, in ), peony betaflexivirus 1 (PeV1, unclassified in ), and peony leafroll-associated virus (PLRaV, in ). PYaCV was 8,666 nucleotides (nt) in length, comprising three open reading frames (ORFs), and shared 63.
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