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A mutant Monascus purpureus strain, M183, which produced monascus pigments (MPs) at 8460 U/g via solid-state batch-fermentation, was generated using the atmospheric and room temperature plasma (ARTP) mutation system. The optimal glucose concentration (80 g/L) in traditional fermentation media that yielded the highest MPs productivity was determined. Response surface methodology (RSM) was applied to maximize MPs production using liquid-state batch-fermentation. Under optimal conditions (0.58 g/L MgSO·7HO, 0.02 g/L ZnSO·7HO, 0.02 g/L FeSO·7HO and 4.85 g/L NHNO), 207.67 U/mL of MPs were produced with 98.12% validity based on the predicted value. Extracellular MPs production increased significantly to 35.52 U/mL, compared to 14.19 U/mL of the original strain, M. purpureus LQ-6. M. purpureus spores immobilized in sodium alginate were studied. A simultaneous fermentation and separation system was established for MPs using the novel absorption resin LX300C to enhance production efficiency of extracellular MPs.
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http://dx.doi.org/10.1016/j.biortech.2018.10.072 | DOI Listing |
Bioresour Technol
September 2025
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, People's Republic of China; Key Laboratory of Deep Processing and Safety Control for Specialty Agricultural Products in Guangxi Universities, Education Department of Guangxi Zhuang Autonomous Region, Nanning 530004,
This study investigated the inhibitory effect of sucrose on the autolysis of recombinant Bacillus subtilis WB600 during keratinase production and elucidated its mechanism. Growth curves, cell morphology observations, cell wall integrity detection, and transcriptome analysis revealed that 2 % sucrose significantly increased cell biomass and delayed autolysis. Keratinase activity reached 5670.
View Article and Find Full Text PDFBioprocess Biosyst Eng
September 2025
Department of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208024, India.
The development of innovative bioprocessing technologies has resulted from the growing global need for sustainable forms of energy and environmentally friendly waste treatment. In this review, we focus on the combined electro-fermentation and microbial fuel cells, as they form a hybrid system that simultaneously addresses wastewater treatment, bioenergy production, and bioplastics. Even though microbial fuel cells produce electricity out of the organic waste by the use of electroactive microorganisms, electro-fermentation improves the microbial pathways through the external electrochemical management.
View Article and Find Full Text PDFJ Vet Med Sci
September 2025
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University.
This study investigated the effects of soy isoflavone yeast fermented extract (soyF) and soy isoflavone yeast unfermented extract (soyN) on rat ileal smooth muscle contraction. SoyF and soyN inhibited carbachol (CCh)- or KCl-induced contraction in a concentration-dependent manner; however, these effects were stronger for CCh-induced contraction than that for KCl, and the relaxation effect was stronger for soyF than for soyN. SoyF-induced relaxation was attenuated by 4-aminopyridine (4-AP), a Kv channel inhibitor, and iberiotoxin (IbTX), a calcium-activated potassium channel (BK channel) inhibitor.
View Article and Find Full Text PDFBioresour Technol
September 2025
Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, China. Electronic address:
This study investigated the effects of five representative biocarriers-biochar (BC), activated carbon (AC), nano-magnetite (NM), zero-valent iron (ZVI), and polyurethane sponge (PUS)-on chain elongation (CE) from ethanol/acetate in anaerobic systems. All carriers enhanced CE to varying extents. BC and NM significantly increased caproate yields (6032.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China. Electronic address:
Chondroitin sulfate (CS), a biopolymer with critical applications in osteoarthritis treatment and biomedical sectors, faces production challenges due to low yields and high costs. This study established a high-yield chondroitin (the major precursor of CS) production platform in Corynebacterium glutamicum for the simultaneous utilization of glucose and xylose from corn straw hydrolysate. Firstly, through codon optimization of genes encoding chondroitin synthase (KfoC) and UDP-N-acetylglucosamine-4-epimerase (KfoA), combined with tailoring metabolic pathways and medium components for chondroitin synthesis, yielded the high-titer strain CgC25.
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