Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background And Aim: Hepatitis B virus (HBV) C/D recombinant is predominant in Tibet in Western China. Although the geographical and ethnic distributions of the C/D recombinant have been described, the clinical implication and the characteristics of viral mutation in the basal core promoter (BCP)/pre-core (PC) region remain unclear.
Methods: A total of 174 chronic HBV carriers, including 115 with chronic hepatitis B, 45 with liver cirrhosis, and 14 with hepatocellular carcinoma, were enrolled. Using next-generation sequencing, the S and BCP/PC genes were determined and analyzed.
Results: Genotypes B, C2, D, and C/D recombinant were detected in 1.1% (2/174), 19.5% (34/174), 0.6% (1/174) and 78.7% (137/174) of the patients, respectively. The clinical parameters and viral mutation frequency in the BCP/PC region were compared between C2- and C/D recombinant-infected patients. The distribution of C2 and C/D did not differ by disease status or liver function. Significantly higher levels of HBV DNA (6.7 ± 1.6 vs. 5.9 ± 1.5, p = 0.014), HBeAg (263.5 vs. 20.0, p = 0.013) and A1762T/G1764A double-mutations (81.0 vs. 61.8%, p = 0.018), but a lower frequency of G1896A stop mutation (33.6 vs. 76.5%, p < 0.001) was observed in patients with the C/D recombinant than in patients with genotype C2. The clonal frequencies of A1762T, G1764A, G1896A and A1846T were lower in patients with C/D than C2.
Conclusion: The C/D recombinant has different mutation pattern in the BCP/PC region compared with genotype C2. The lower clonal frequencies of BCP/PC mutations may explain the higher levels of HBV DNA and HBeAg in C/D-infected patients.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12072-018-9885-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tempting fate: Neural crest induction along the body axis.
Cells Dev
February 2025
California Institute of Technology, Division of Biology and Biological Engineering, Pasadena 91125, USA. Electronic address:
Neural crest induction begins at the neural plate border and involves the intricate interplay of signaling and transcriptional events. In this review, we examine the literature on neural crest induction, focusing primarily on the chick model due to the extended time during which the induction process occurs. While it is well-established that induction initiates during mid-gastrulation, evidence from tissue recombination and transcriptomic analyses suggests that the process continues until neural tube closure.
View Article and Find Full Text PDFAdaptive patterning of vascular network during avian skin development: Mesenchymal plasticity and dermal vasculogenesis.
Cells Dev
September 2024
Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, United States of America. Electronic address:
Article Synopsis
- The vasculature network supplying blood to feather buds in developing chicken skin forms primarily through local vasculogenesis and then connects to the central vascular system.
- Observations using transgenic Japanese quail indicate that vascular progenitor cells emerge after feather primordia have formed and that vasculature from each bud links with neighboring structures before integrating with the central vasculature.
- The research demonstrates that endothelial cells from the skin are more similar to skin dermal cells than those from the aorta, highlighting the significance of mesenchymal plasticity and differentiation in development and potential regeneration processes.
Inhibition of TGFβ1/Smad pathway by NF-κB induces inflammation leading to poor wound healing in high glucose.
Cells Dev
December 2022
Department of Orthopedics, Ningxia Hui Autonomous Region People's Hospital (The First Affiliated Hospital of Northwest University for Nationalities), Yinchuan, China. Electronic address:
This study mainly analyzed the relationship between nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor-β (TGFβ1)/Smad under high glucose environment and its influence on wound healing. Fibroblast NIH-3T3 was used to analyze the effect of high concentration glucose (20 nmol/mL) on cell viability, migration ability, inflammation level and NF-κB pathway. Pyrrolidinedithiocarbamate (PDTC) was used to inhibit NF-κB for rescue experiments.
View Article and Find Full Text PDF