Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Compared to animals, evolution of plant calcium (Ca) physiology has led to a loss of proteins for influx and small ligand-operated control of cytosolic Ca, leaving many Ca mechanisms unaccounted for. Here, we show a mechanism for sorting and activation of glutamate receptor-like channels (GLRs) by CORNICHON HOMOLOG (CNIH) proteins. Single mutants of pollen-expressed GLRs (GLRs) showed growth and Ca flux phenotypes expected for plasma membrane Ca channels. However, higher-order mutants of GLR3.3 revealed phenotypes contradicting this assumption. These discrepancies could be explained by subcellular GLR localization, and we explored the implication of CNIHs in this sorting. We found that GLRs interact with CNIH pairs, yielding specific intracellular localizations. CNIHs further trigger GLR activity in mammalian cells without any ligand. These results reveal a regulatory mechanism underlying Ca homeostasis by sorting and activation of GLRs by CNIHs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1126/science.aar6464 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reduced circulating regulatory T cells in primary Sjögren's syndrome: the contribution of enhanced apoptosis and impaired survival.
Front Immunol
September 2025
Division of Rheumatology, Department of Medicine, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Background: Regulatory T cells (Tregs) are found to be critical for maintaining immune tolerance to self-antigens; however, their status in primary Sjögren's syndrome (pSS) remains unclear. We investigated alterations in the abundance of peripheral Tregs in a large pSS cohort and their implications for patients.
Methods: Levels of CD4+CD25+FOXP3+Treg cells in the peripheral blood of 624 patients with pSS, and 93 healthy controls (HCs) were detected using modified flow cytometry (FCM).
attenuates acute lung injury by regulating the differentiation and function of myeloid-derived suppressor cells.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
May 2025
Department of Pathology, First Clinical College, Changzhi Medical College, Changzhi 046000.
Objectives: Acute lung injury (ALI) is an acute respiratory failure syndrome characterized by impaired gas exchange. Due to the lack of effective targeted drugs, it is associated with high mortality and poor prognosis. (TW) has demonstrated anti-inflammatory activity in the treatment of various diseases.
View Article and Find Full Text PDFA Low-Voltage-Driven Droplet Sorter for High-Stability and Small-Deformation Droplet Sorting.
Electrophoresis
September 2025
School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacturing of Precision Medicine Equipment, Southeast University, Nanjing, China.
Electric droplet sorting is widely applied in the screening of target molecules, cells, drugs, and microparticles. Previous studies have made several optimizations on the electrode materials, structures, and arrangements. However, voltages of over 1 kV are required to realize droplet sorting, which causes the undesired droplet splitting.
View Article and Find Full Text PDFSperm activation for fertilization requires robust activity of the TAT-5 lipid flippase.
Dev Biol
September 2025
Department of Biological Sciences, University of Denver, Denver, CO 80208, USA; Department of Biochemistry and Biophysics, AgriLife Research, Texas A&M University, College Station, TX 77843, USA. Electronic address:
During fertilization, sperm and egg membranes signal and fuse to form a zygote and begin embryonic development. As lipids participate in signaling and membrane fusion, we investigated the role of lipid asymmetry in gametogenesis, fertilization, and embryogenesis. We show that the lipid flippase TAT-5, an essential P4-ATPase that maintains phosphatidylethanolamine asymmetry, is required for both oocyte formation and sperm activation, albeit at different levels of flippase activity.
View Article and Find Full Text PDFObesity hinders the efficacy of adipose-derived stem cells for knee osteoarthritis by reducing the proportion of DPP4+ stem cells.
Stem Cells Transl Med
July 2025
Department of Joint Surgery and Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China.
Osteoarthritis (OA) is the most prevalent and disabling joint disease, while adipose-derived stem cells (ASCs) have emerged as a promising therapeutic option in pre-clinical studies. However, the therapeutic efficacy of ASCs may be influenced by the source of these cells, especially in obese patients. This study compared the effects of intra-articular injections of ASCs from wild-type (WT) and ob/ob (OB) mice.
View Article and Find Full Text PDF