Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Excessive fluoride in natural water ecosystem has the potential to detrimentally affect amphibians, but little is known of such effects or underlying mechanisms in Bufo gargarizans embryos. In the present study, the effects of fluoride exposure on B. gargarizans embryos were investigated. First, fluoride teratogenic experiment showed that the 9 days EC of fluoride on B. gargarizans embryos was 177.62 mg/L. Then, we studied the sublethal effects of fluoride on B. gargarizans embryos at control, 0.7, 4.1, 19.6, 41.9, and 62.7 mg/L fluoride concentration. Malformation, growth, and development of embryos were monitored, and type 2 and 3 iodothyronine deiodinase (Dio2 and Dio3), thyroid hormone receptors (TRα and TRβ) mRNA levels were measured. Our results showed the morphological malformations, such as tail curvature (lordosis), edema, cuticularized ciliated cells, and hyperplasia were occurred during fluoride exposure. Growth and development were all inhibited at 19.5, 41.9, and 62.7 mg/L fluoride-treated groups after 9 days' exposure. According to real-time PCR results, exposure to fluoride upregulated Dio3 and TRβ mRNA expression and downregulated Dio2 and TRα mRNA level. All above indicated that excessive fluoride could induce morphology malformations, inhibit embryonic growth and development, and disrupt the normal function of maternal thyroid hormone in B. gargarizans embryos. Environ. Mol. Mutagen. 59:123-133, 2018. © 2017 Wiley Periodicals, Inc.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/em.22147 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bufadienolide-Fatty Acid Conjugates from the Fertilized Eggs of Toad : Isolation, Characterization, Toxicity, and Antiproliferative Evaluation.
J Agric Food Chem
August 2024
Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Discovery of Chinese Ministry of Education (MOE), Guangzhou City Key Laboratory of Precision Chemical Drug Development, College of Ph
Bufadienolides (BDs) are a class of naturally occurring toxins present in amphibian toads. Serving as the chemical weapons, they exist not only in the adult toads but also in toad eggs. Guided by mass spectrometry (MS)-based component analysis and feature-based molecular networking (FBMN), 30 bufadienolide-fatty acid conjugates (BDFs) were isolated from the fertilized eggs of toad , including 25 previously undescribed compounds (-).
View Article and Find Full Text PDFEffects of ciprofloxacin and levofloxacin on initial colonization of intestinal microbiota in Bufo gargarizans at embryonic stages.
Chemosphere
August 2024
College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. Electronic address:
Ciprofloxacin (CIP) and levofloxacin (LEV) are broad-spectrum antibiotics with potent antibacterial activity. Although many studies have shown that antibiotics can lead to gut microbiota disruption, the effects of CIP and LEV on gut microbial colonization at the embryonic stage remain poorly characterized. Here, we evaluated the response of Bufo gargarizans embryos in terms of gut microbiota colonization, growth and developmental stages to CIP and LEV exposure.
View Article and Find Full Text PDFMorphological and Transcriptomic Analyses Reveal the Toxicological Mechanism and Risk of Nitrate Exposure in Embryos.
Animals (Basel)
March 2024
Life and Environmental Science College, Wenzhou University, Wenzhou 325003, China.
In recent years, nitrate (NO-N) pollution in water bodies has been increasing due to the excessive use of nitrogen-based fertilizers. Exposure to NO-N during the development of amphibian embryos may have lasting effects on the growth and development of individuals and even threaten their survival, but the toxicity mechanism of NO-N in amphibian embryos prior to thyroid morphogenesis remains unclear. In the present study, was selected as the model organism to investigate the toxic effects of 10 mg/L and 100 mg/L NO-N exposure (N10 and N100) on amphibian embryos using methimazole (MMI) and exogenous thyroxine (T4) as the reference groups.
View Article and Find Full Text PDFMolecular phylogeny of the family Rhabdiasidae (Nematoda: Rhabditida), with morphology, genetic characterization and mitochondrial genomes of Rhabdias kafunata and R. bufonis.
Parasit Vectors
March 2024
Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, Hebei, People's Republic of China.
Article Synopsis
- The Rhabdiasidae family includes over 110 species of nematode parasites that predominantly affect amphibians and reptiles, but their evolutionary relationships and classification within the order Rhabditida are not well understood.
- Recent research involved identifying two specific species from the Rhabdiasidae family, conducting both morphological studies and molecular techniques, including the sequencing of their mitochondrial genomes for the first time.
- Findings revealed unique morphological details of the two species, confirmed the composition of their mitochondrial genomes, and demonstrated distinct gene arrangements, which provide new insights into the systematics and evolutionary relationships within the family Rhabdiasidae.
Effects of Separate and Combined Exposure of Cadmium and Lead on the Endochondral Ossification in Bufo gargarizans.
Environ Toxicol Chem
May 2022
School of Water and Environment, Chang'an University, Xi'an, China.
Cadmium (Cd) and lead (Pb) are ubiquitous in aquatic environments and most studies have examined the potential effects of Cd or Pb alone on aquatic organisms. In the present study, chronic effects of Cd and Pb, alone and in combination, on Bufo gargarizans were investigated by exposing embryos to these contaminants throughout metamorphosis. Significant reductions in body mass and snout-to-vent length were observed in B.
View Article and Find Full Text PDF