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Article Abstract
Objective: This study aimed to investigate the cytotoxicity, apoptosis induction, and mechanism of action of steviol on human breast cancer cells (Michigan Cancer Foundation-7 [MCF-7]).
Materials And Methods: Sulforhodamine-B assay was performed to analyze cytotoxic potential of Steviol whereas flow cytometer was used to analyze cell cycle, apoptosis, and reactive oxygen species generation.
Results: Studying the viability of cells confirms the IC of Steviol in MCF-7 cells which was 185 μM. The data obtained from fluorescence-activated cell sorter analysis reveal Steviol-mediated G2/M-phase arrest ( < 0.05) in addition to the presence of evident sub-G0/G1 peak ( < 0.05) in the MCF-7 cells, signifying the ongoing apoptosis.
Conclusion: Thus, results suggest that induction of apoptosis in MCF-7 cells was due to dose-dependent effect of Steviol. Our first findings indicate Steviol as a promising candidate for the treatment of breast cancer.
Summary: Steviol remarkably inhibited the growth MCF-7 HBCCs in a dose dependent mannerIt abolishes cell cycle progression by arresting cells at G2/M phaseSteviol induces the cells to undergo apoptosisSteviol induces the cells to generate reactive oxygen species (ROS). MCF-7: Michigan Cancer Foundation-7; SRB: Sulforhodamine-B assay; FACS: Fluorescence-activated cell sorter; ROS: Reactive oxygen species; DNA: Deoxyribonucleic acid.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551348 | PMC |
| http://dx.doi.org/10.4103/pm.pm_29_17 | DOI Listing |
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