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Article Abstract
Summary: We present 'Threshold-seq,' a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule in a given dataset before it can be considered different from 'background.' The proposed scheme is easy to implement and incorporate into existing pipelines.
Availability And Implementation: Source code of Threshold-seq is freely available as an R package at: http://cm.jefferson.edu/threshold-seq/.
Contact: isidore.rigoutsos@jefferson.edu.
Supplementary Information: Supplementary data are available at Bioinformatics online.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870860 | PMC |
| http://dx.doi.org/10.1093/bioinformatics/btx073 | DOI Listing |
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