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The oxidation ditch process is one of the most economical approaches currently used to simultaneously remove organic carbon, nitrogen, and also phosphorus (P) from wastewater. However, limited information is available on biological P removal in this process. In the present study, microorganisms contributing to P removal in a full-scale oxidation ditch reactor were investigated using culture-dependent and -independent approaches. A microbial community analysis based on 16S rRNA gene sequencing revealed that a phylotype closely related to Dechloromonas spp. in the family Rhodocyclaceae dominated in the oxidation ditch reactor. This dominant Dechloromonas sp. was successfully isolated and subjected to fluorescent staining for polyphosphate, followed by microscopic observations and a spectrofluorometric analysis, which clearly demonstrated that the Dechloromonas isolate exhibited a strong ability to accumulate polyphosphate within its cells. These results indicate the potential key role of Dechloromonas spp. in efficient P removal in the oxidation ditch wastewater treatment process.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5158118 | PMC |
http://dx.doi.org/10.1264/jsme2.ME16097 | DOI Listing |
Ying Yong Sheng Tai Xue Bao
July 2025
School of Ecology and Environment, Ningxia University, Yinchuan 750021, China.
Ammonia oxidation plays a critical role in nitrogen cycling within riparian zones. To investigate this process in saline-alkali soils of the Yinbei region, northern Yinchuan, Ningxia, we selected five distinct riparian types along the Third Drainage Ditch: gravel-reed mixed zone, reed zone, high-salt zone, embankment zone and bare soil zone. We quantified soil potential nitrification rates (PNR), environmental factors, and analyzed ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities via me-tagenomics and qPCR targeting genes.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
August 2025
College of Agricultural Science and Engineering, Hohai University, Nanjing 210098, China.
Coastal reclamation area is an agricultural region formed by artificial transformation of coastal tidal flats. Changes in land use types would reshape regional carbon cycling patterns. Methane (CH) is a short-lived greenhouse gas with a high global warming potential, the emission of which is a key contributor to the accelerated global climate change.
View Article and Find Full Text PDFSci Rep
August 2025
MARA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Rice-crayfish farming represents a typical green and low-carbon alternative to rice monoculture. It is important to investigate the carbon sequestration and emission reduction effect of rice-crayfish farming to improve paddy soil quality, ensure food security, and address climate change challenges. In this study, we systematically evaluated the carbon sequestration and emission reduction effects of rice-crayfish farming through field experiment, carbon footprint assessment, and the DeNitrification-DeComposition (DNDC) model.
View Article and Find Full Text PDFHuan Jing Ke Xue
June 2025
College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China.
Wastewater treatment plants (WWTPs) are considered to be the major source of microplastics entering natural water bodies, and research on microplastics in developing countries is still in its infancy. Taking Handan, a typical city in northern China, as the object, the characteristics of the removal rate of microplastics in the whole process of the first phase of a sewage treatment plant and the expansion project were studied. The results showed that: ① The shape of the wastewater microplastics was mainly fibrous microplastics, and the size of the wastewater microplastics was less than 0.
View Article and Find Full Text PDFJ Environ Manage
August 2025
Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan. Electronic address:
Digital polymerase chain reaction (dPCR) is a significant advancement in health-related water microbiology, enabling absolute quantification without standard curves. By partitioning samples into thousands of individual reactions, dPCR allows for precise quantification even in the presence of inhibitory substances common in environmental samples. This study evaluated the applicability of dPCR to detect gastroenteritis-causing enteropathogens (Salmonella spp.
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