Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
On-line high performance liquid chromatography (HPLC) coupled with three biochemical detection (BCD) methods was applied to evaluate bioactive components in Danshen injection. On-line HPLC-photo-diode array-fluorescence detection based on the fluorogenic substrate 7-acetoxy-1-methyl quinolinium iodide, was built to search acetylcholinesterase (AChE) inhibitors in Danshen injection. On-line HPLC coupled with the scavenging assay of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals was developed to screen antioxidants. The three active profiles were obviously different. Radical scavenging profiles revealed seven strong peaks in the chromatographic fingerprint possessing obvious free radical inhibition effects, while some minor peaks exhibited stronger AChE inhibition activities. The main radical scavengers and AChE inhibitors were identified by HPLC-MS. Several unknown ingredients showing strong AChE inhibition activities needed further identification except protocatechuic aldehydrate, salvianolic acid H or I and lithospermic acid. The on-line multiple on-line HPLC-BCD methods will provide powerful tools in the field of pharmacognosy for fast-track identification of interesting and/or novel bioactive compounds.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bmc.3772 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative analysis of phenolic compounds and triterpenoid saponins in edible plant parts of Acanthopanax sessilflorus using UPLC-MS and multivariate analyses.
Food Res Int
November 2025
Department of Food Sciences, National Institute of Crop and Food Sciences, Rural Development Administration, 55365 Wanju, Republic of Korea. Electronic address:
Acanthopanax sessiliflorus, belonging to the Araliaceae family, is used as medicinal herbs and dietary supplements, and can be consumed as seasoned vegetables, salads, pickles, functional tea, and wine. Their edible parts (shoots, leaves, fruis, and stems) are considered as a highly valuable food source with health benefits. The comparison of the qualitative and quantitative characteristics of functional compounds in these plant parts is still limited.
View Article and Find Full Text PDFFlavonoid compound from leaves inhibits adenovirus infection related to cell cycle based on UHPLC-Q-Exactive-Orbitrap/MS and experimental validation.
Front Cell Infect Microbiol
September 2025
Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Introduction: leaves (FSL), a traditional Chinese ethnomedicinal herbal material used to prepare health-promoting infusions and pharmacologically noted for their robust anti-inflammatory, antioxidant, and broad-spectrum antiviral activities, nevertheless have an as-yet-uncharacterized molecular mechanism of action against human adenovirus (HAdV).
Methods: Ultra-high-performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive-Orbitrap/MS) was employed to identification of FSL components. Publicly available GEO datasets were mined to identify HAdV-associated differentially expressed genes (DEGs).
A rapidly synthesized magnetic microporous organic network for ultrasensitive detection of non-steroidal anti-inflammatory drugs in fish.
J Chromatogr A
September 2025
Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, Zhejiang, China. Electronic address:
Non-steroidal anti-inflammatory drugs (NSAIDs) residues pose a potential threat to aquatic ecosystems and food safety. In this work, novel imine bond/pyridine nitrogen-rich magnetic microporous organic networks (MMONs-Br and MMONs-I) were synthesized via a facile one-pot strategy using brominated and iodinated precursors for the detection of NSAIDs in fish. Brunauer-Emmett-Teller analysis revealed a striking 11-fold difference in specific surface areas between the two materials (MMONs-Br: 293.
View Article and Find Full Text PDFReliable and sensitive ultra-short chain per- and polyfluorinated alkyl substances (PFAS) analysis in food: a polar reverse-phase chromatography approach.
J Chromatogr A
September 2025
Istituto Nazionale di Ricerca Metrologica (INRIM), Strada delle cacce 91, Turin, 10135, Italy.
Food contamination by per- and polyfluoroalkyl substances (PFAS), especially ultra-short-chain (USC) compounds, poses a growing concern due to their environmental persistence and potential health risks. Despite the developing regulatory framework, analytical challenges persist in quantifying polar USC-PFAS in complex content food matrices. This study presents the development and validation of a novel high-performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) method for the accurate determination of USC-PFAS (carbon chain length from one to four, C1-C4) in tomato-based products (i.
View Article and Find Full Text PDFQuantification of carbohydrates in sugar beet roots by using ultra-fast liquid chromatography coupled with time of flight mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci
September 2025
Edward T. Schafer Agricultural Research Center, USDA ARS, Fargo, ND, USA.
The quantification of sucrose and other carbohydrates in sugar beet roots is essential prior to their processing to assess sugar production yield. In this study, a rapid, highly sensitive and selective ultra-fast liquid chromatography coupled with time of flight mass spectrometry (UFLC-ToFMS) method was developed and validated for the simultaneous analysis of monosaccharides (fructose, glucose-galactose), a disaccharide (sucrose), and a trisaccharide (raffinose). The method showed 1000-fold higher sensitivity, with LOD and LOQ ranging between 0.
View Article and Find Full Text PDF