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http://dx.doi.org/10.1371/journal.pgen.1005958 | DOI Listing |
Front Genome Ed
August 2025
University of Massachusetts School of Medicine, Worcester, MA, United States.
CRISPR-Cas9 is a breakthrough genome-editing platform that can cut chosen DNA sequences with unprecedented speed, accuracy, and affordability. By reprogramming a single guide RNA, researchers now alter gene function, correct pathogenic variants, or introduce novel traits. Earlier tools such as zinc-finger nucleases and TALENs performed similar tasks but were significantly more complex and costly.
View Article and Find Full Text PDFCrit Rev Oncol Hematol
September 2025
Student Research Committee, Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.. Electronic address:
Epigenetic regulation is fundamental to hematopoiesis, influencing stem cell fate, lineage commitment, and the development of hematologic diseases. Recent technological innovations have transitioned from traditional genetic editing towards programmable, reversible epigenetic modulation without altering the DNA sequence. This review explores the evolution of epigenetic editing platforms, from zinc finger proteins and TALEs to the transformative CRISPR-dCas9 system, and introduces next-generation technologies leveraging dCas12, dCas13, and modular RNA-guided systems.
View Article and Find Full Text PDFFront Immunol
July 2025
Department of Oncology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, China.
[This corrects the article DOI: 10.3389/fimmu.2025.
View Article and Find Full Text PDFPlant Mol Biol
July 2025
National Key Laboratory for Tropical Crop Breeding, Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, 572024/571101, Sanya, Hainan, Haikou, China.