Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.

J Struct Biol

Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; The Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. Electronic address:

Published: November 2015


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Article Abstract

The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633381PMC
http://dx.doi.org/10.1016/j.jsb.2015.08.015DOI Listing

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