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As the magnetic storage density increases in commercial products, e.g. the hard disc drives, a full understanding of dynamic magnetism in nanometer resolution underpins the development of next-generation products. Magnetic force microscopy (MFM) is well suited to exploring ferromagnetic domain structures. However, atomic resolution cannot be achieved because data acquisition involves the sensing of long-range magnetostatic forces between tip and sample. Moreover, the dynamic magnetism cannot be characterized because MFM is only sensitive to the static magnetic fields. Here, we develop a side-band magnetic force microscopy (MFM) to locally observe the alternating magnetic fields in nanometer length scales at an operating distance of 1 nm. Variations in alternating magnetic fields and their relating time-variable magnetic domain reversals have been demonstrated by the side-band MFM. The magnetic domain wall motions, relating to the periodical rotation of sample magnetization, are quantified via micromagnetics. Based on the side-band MFM, the magnetic moment can be determined locally in a volume as small as 5 nanometers. The present technique can be applied to investigate the microscopic magnetic domain structures in a variety of magnetic materials, and allows a wide range of future applications, for example, in data storage and biomedicine.
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http://dx.doi.org/10.1038/srep05594 | DOI Listing |
Neuroimage Rep
September 2025
Arizona State University, Tempe, AZ, 85287, USA.
Non-intrusive neuroimaging technology offers fast and robust diagnostic tools for neuro-disorder disease diagnosis, such as Attention-Deficit/Hyperactivity Disorder (ADHD). Resting-state functional magnetic imaging (rs-fMRI) has been demonstrated to have great potential for such applications due to its unique capability and convenience in providing spatial-temporal brain imaging. One critical challenge of using rs-fMRI data is the high dimensionality for both spatial and temporal domains.
View Article and Find Full Text PDFProtein Expr Purif
September 2025
Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam; Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam. Electronic addr
The 3C-like protease (3CLpro) of SARS-CoV-2 is a crucial target for antiviral drugs due to its essential role in viral polyprotein processing. In this study, we designed and produced a modular fluorescent recombinant substrate (6×His-ECFP-AVLQSGFRK-EYFP), which was then immobilized on Ni-NTA magnetic beads (Ni-NTA-6×His-ECFP-AVLQSGFRK-EYFP) for the assay of 3CLpro activity. Upon cleavage at the specific AVLQ↓SG motif, the EYFP fragment was released into the supernatant and quantified via fluorescence measurement (Ex/Em = 480/528 nm).
View Article and Find Full Text PDFNeuroimage
September 2025
The Clinical Hospital of Chengdu Brain Science Institute, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, P.R. China; Brain-Computer Interface & Brain-Inspired Intelligence Key Laboratory of Sichuan Province, University of Electronic
Functional magnetic resonance imaging (fMRI) opens a window on observing spontaneous activities of the human brain in vivo. However, the high complexity of fMRI signals makes brain functional representations intractable. Here, we introduce a state decomposition method to reduce this complexity and decipher individual brain functions at multiple levels.
View Article and Find Full Text PDFArch Gerontol Geriatr
August 2025
China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. Electronic address:
Post-stroke cognitive impairment (PSCI) imposes a significant economic and social burden on patients and their families. High-density lipoprotein cholesterol (HDL-C) is reported to have protective effects on cognitive function in older adults. This study assesses the effects of HDL-C during the acute period of stroke on PSCI.
View Article and Find Full Text PDFBrain Stimul
September 2025
Department of Philosophy, University of Milan, Milan, via Festa Del Perdono, 7, 20122, Italy; Cognition in Action (CIA) Unit, PHILAB, University of Milan, Via Santa Sofia, 9, 20122, Italy. Electronic address:
Background: To investigate covert motor processes, transcranial magnetic stimulation (TMS) studies often use motor-evoked potentials (MEPs) as a proxy for inferring the state of motor representations. Typically, these studies test motor representations of actions that can be produced by the isolated contraction of one muscle, limiting both the number of recorded muscles and the complexity of tested actions. Furthermore, univariate analyses treat MEPs from different muscles as independent, overlooking potentially meaningful intermuscular relationships encoded in MEPs amplitude patterns at the single-trial level.
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