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Because existing human experience diagnosis method and apparatus for auxiliary diagnosis method are difficult to diagnose quickly engine knock. Atomic absorption spectrometry was used to detect the automobile engine knock in in innovative way. After having determined Fe, Al, Cu, Cr and Pb content in the 35 groups of Audi A6 engine oil whose travel course is 2 000 -70 000 kilometers and whose sampling interval is 2 000 kilometers by atomic absorption spectrometry, the database of primary metal content in the same automobile engine at different mileage was established. The research shows that the main metal content fluctuates within a certain range. In practical engineering applications, after the determination of engine oil main metal content and comparison with its database value, it can not only help to diagnose the type and location of engine knock without the disintegration and reduce vehicle maintenance costs and improve the accuracy of engine knock fault diagnosis.
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PLoS Pathog
September 2025
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Neuroinflammation within the central nervous system (CNS) is recognized as a critical pathological process in meningitic Escherichia coli (E. coli) infection, leading to severe neurodegenerative disorders and long-term sequelae. Astrocyte reactivity plays a pivotal role in driving the neuroinflammatory cascade in response to pathological stimuli from peripheral sources or other cellular components of the CNS.
View Article and Find Full Text PDFNat Cell Biol
September 2025
Department of Medicine, Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Durotaxis, cell migration along stiffness gradients, is linked to embryonic development, tissue repair and disease. Despite solid in vitro evidence, its role in vivo remains largely speculative. Here we demonstrate that durotaxis actively drives disease progression in vivo in mouse models of lung fibrosis and metastatic pancreatic cancer.
View Article and Find Full Text PDFBiotechnol Bioeng
September 2025
Department of Biosystems Engineering, Auburn University, Auburn, Alabama, USA.
Ensuring sufficient crop yields in an era of rapid population growth and limited arable land requires innovative strategies to enhance plant resilience and sustain, or even improve, growth and productivity despite environmental stress. Besides symbiotic nitrogen fixation, rhizobia may play a central role in sustainable agriculture by alleviating the detrimental effects of ethylene-a key stress hormone in plants-especially under conditions like drought through the deamination of 1-aminocyclopropane-1-carboxylic acid (ACC). In this study, we focused on genetically engineering a new Bradyrhizobium sp.
View Article and Find Full Text PDFCell Rep
September 2025
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Peter O'Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern M
Myelination is essential for normal brain function, yet the mechanisms governing neuron-oligodendrocyte interactions that ensure proper myelination levels remain poorly understood. Here, we identify transcription factor EB (TFEB) as a molecular link that connects extrinsic neuronal cues to intrinsic oligodendrocyte transcriptional programs, regulating central nervous system myelination. Using a TFEB epitope-tagged knock-in mouse model, we find that neurons sequester most of the TFEB protein in the cytoplasm of myelinating oligodendrocytes.
View Article and Find Full Text PDFGene
September 2025
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China. Electronic address:
Komagataella phaffii is a famous microbial host in synthetic biology field for expressing heterologous proteins under alcohol oxidase 1 promoter (P). This study undertook two successive rounds of gene fragment knockout, identifying a 13 bp segment, termed D3, as a negative cis-acting element in the 5' untranslated region of P. Notably, upon knockout of the D3 fragment, the expression of green fluorescent protein (GFP), serving as the model for heterologous protein expression, improved by 3.
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