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The experiment was carried out in a sequencing batch reactor (SBR), using granular sludge with 90% shortcut nitrification accumulation ratio, which had been cultivated by the laboratory to seed the reactor. The effects of temperature on characteristics, stability, nitrogen conversion properties and activity of short-cut nitrification granular sludge were investigated. The results show that the temperature has a significant influence on structure and short-cut nitrification performance of short-cut nitrification granular sludge. 30 degrees C was the optimum temperature for shortcut nitrification granular sludge cultivation, and the granules were more compact which led better flocculation and settleability. SVI and average diameter of sludge reached up to 39 mL x g(-1) and 3.03 mm, respectively. The nitrification accumulation was maintained at 96.17% during this period. When the temperature was at 25 degrees C, the granules changed to a loose-structure along with the electronegativity increased as well as the hydrophobicity decreased, which was due to the increase of EPS and decreased ratio of protein to polysaccharide. The granules disintegrated during this period, at the same time, the nitrification accumulation ratio was below 35%. It can be seen that the shortcut nitrification performance was destroyed. At 15 degrees C, the shortcut nitrification sludge also disintegrated and had a looser structure. However, the oxygen penetration depth increased at a lower temperature, which made the relative quantity of ammonia oxidizing bacteria (AOB) in reactor increased, the nitrification accumulation was around 68% ultimately.
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Environ Technol
August 2025
School of Water and Environment, Chang'an University, Xi'an, People's Republic of China.
Nitric oxide (NO) and nitrous oxide (NO) are significant greenhouse gases, yet their production mechanisms remain inadequately understood. To elucidate the production mechanisms of NO and NO during biological nitrogen removal, the variations in dissolved NO and NO concentrations during autotrophic shortcut nitrification were analysed in a sequencing batch reactor. The findings indicated that increasing the influent ammonium concentration from 120 mg-N/L to 240 mg-N/L led to a transition of complete nitrification to shortcut nitrification, achieving >80% nitrite accumulation.
View Article and Find Full Text PDFBioresour Technol
November 2025
Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:
Air pollution and nitrogen contamination residues remain challenges in the conventional biological treatment of industrial wastewater containing volatile organic compounds. In this study, counter-diffusion biofilms were integrated with shortcut nitrification-denitrification technology to enhance pyridine biodegradation through shortcut pyridine-N transformation (EPB-SPNT), with emphasis on biofilm stratification and microbial spatial interactions. Results showed that the removal efficiency of pyridine and total nitrogen reached 100 % and 91.
View Article and Find Full Text PDFJ Environ Manage
August 2025
School of Environmental Science and Technology, Suzhou University of Science and Technology, Suzhou, 215009, China.
Double short-cut sulfur autotrophic denitrification (DSSADN) is capable of reconverting NO-N produced by partial nitrification -Anammox (PN/Anammox) into NO-N, while simultaneously reducing SO byproduct pollution. Understanding the nitrogen and sulfur transformation processes as well as the functional enzyme activity changes in DSSADN under varying pH conditions is crucial for achieving synchronous accumulation of S and NO-N. This study investigated the directional accumulation characteristics of S and NO-N in sulfur autotrophic denitrification (SADN) under different pH conditions by directionally adjusting the pH within the SADN system.
View Article and Find Full Text PDFJ Environ Manage
August 2025
College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004, China. Electronic address:
To explore how biochar influences nitrogen cycling in unsaturated, capillary, and saturated zones of partially saturated vertical flow constructed wetlands (VFCWs), three parallel VFCWs were established to examine the effects of biochar's better water holding capacity on nitrogen removal and NO emissions. Microbial mechanisms involved were studied by conducting ETS activity, metagenomic sequencing and performing high-throughput sequencing of 16S rRNA. Results indicated that the combination of adding 40 % biochar and maintaining water level of 45 cm facilitated TN removal and suppressed NO emissions, achieving TN removal efficiency of 73.
View Article and Find Full Text PDFWater Res
September 2025
Department of Environmental Systems Engineering, Korea University Sejong Campus, Sejong City, South Korea. Electronic address:
This study investigates the membrane-aerated biofilm photoreactor (MABPR) for treating aquaculture effluents with low C/N ratio and elevated salinity (0.5%-3.2%).
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