Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: To explore the molecular mechanism of continuous monoculture problem by constructing the cDNA libraries of continuous monoculture Rehmannia glutinosa.
Method: To use the suppression subtractive hybridization (SSH) technique to construct the forward and reverse subtractive cDNA libraries of continuous monoculture R. glutinosa to adopt blue-white colony screening and PCR to detect the positive clones which would be sequenced and analyzed by bioinformatics.
Result: The subtracted cDNA libraries of continuous monoculture R. glutinosa. were successfully constructed, and the result showed that the forward and reverse subtracted libraries obtained 300 positive clones, respectively. The forward and reverse libraries got different ESTs, and produced 232 (forward library) and 214 (reverse library) unique ESTs by sequencing. Based on homology search of BLASTX and BLASTN in NCBI, 200 and 195 of unique ESTs were homologous to known genes in the forward and reverse libraries, respectively. Categories of orthologous group (COG) showed that the forward and reverse libraries got 60 and 61 ESTs with the corresponding gene annotation, involving 21 metabolic pathways.
Conclusion: The information of differential expression genes in continuous monoculture R. glutinosa, and their functional annotation of differentially expressed genes indicate that continuous monoculture has a profound effect on expression of the genes in R. glutinosa. Furthermore, the research analyzed several key genes in response to replant problem, which provided a foundation for revealing the molecular mechanism of continuous monoculture R. glutinosa.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Depth-dependent effects of crop rotation and monoculture on dissolved organic matter quantity and quality.
Front Plant Sci
August 2025
Department Soil Science and Environmental Analyses, Institute of Soil Science and Plant Cultivation-State Research Institute, Puławy, Poland.
Introduction: Soil dissolved organic matter (DOM) regulates nutrient cycling and carbon sequestration, yet how cropping systems (rotation vs. monoculture) shape the vertical distribution and molecular traits of DOM remains unclear.
Methods: We leveraged a long-term experiment (est.
Phenolic acid reduction in rhizosphere via organic fertilization.
Plant Signal Behav
December 2025
Hubei Key Laboratory of Spices & Horticultural Plant Germplasm Innovation & Utilization, College of Horticulture and Gardening, Yangtze University, Jingzhou, China.
is a valuable traditional Chinese medicinal plant that is prone to germplasm degradation during long-term continuous monoculture. Allelopathic autotoxicity, which is mediated primarily by phenolic acids, is considered a major factor contributing to this degradation. To reveal the accumulation patterns of phenolic acids in the rhizospheric soil of under continuous monoculture, five phenolic acids (-hydroxybenzoic acid, vanillic acid, syringic acid, -coumaric acid, and ferulic acid) in the rhizospheric soil of across 1-5 y, and various fertilizer regimes (chemical fertilizer, chemical fertilizer + organic fertilizer, and organic fertilizer) were determined to assess their accumulation characteristics, along with soil fertility parameters.
View Article and Find Full Text PDFMechanism of soil microbial community degradation under long-term tomato monoculture in greenhouse.
Front Microbiol
July 2025
College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, China.
Tomato ( L.), an economically significant crop, is frequently cultivated in greenhouses under continuous monoculture systems. Motivated by intensive agricultural practices and economic incentives, continuous cropping has become prevalent in China, yet it often results in soil degradation, including nutrient imbalances and microbial community shifts.
View Article and Find Full Text PDFSERS based pH nanosensors forpH measurement in multicellular 3D tumour spheroids.
Biomed Mater
August 2025
Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
Tumor acidosis is a consequence of altered metabolism that primarily takes place due to lactate secretion from anaerobic glycolysis. As a result, many regions within the tumors are chronically hypoxic and acidic. To measure the intratumor pH dynamically, we have fabricated a biocompatible pH nanoparticle sensor using surface-enhanced Raman spectroscopy (SERS-pNPS) and monitored continuous pH levels in three-dimensional multicellular spheroids.
View Article and Find Full Text PDFMulticellular hepatic in vitro models using NANOSTACKS: human-relevant models for drug response prediction.
In Vitro Model
August 2025
Sci-Tech Daresbury, REVIVOCELL Limited, Keckwick Lane, Daresbury, Warrington WA4 4 AD UK.
Drug-induced liver injury (DILI) continues to be one of the leading causes of drug attrition during clinical trials, as well as the number one cause of post-market drug withdrawal due to the limited predictive accuracy of preclinical animals and conventional in vitro models. In this study, the NANOSTACKS platform was introduced as a novel in vitro tool to build in vivo-relevant organ models for predicting drug responses. In particular, hepatic models, including monocultures of primary human hepatocytes (PHH), tricultures of PHH, human stellate cells (HSC), and human liver endothelial cells (LECs), and tetracultures of PHH, HSC, LECs, and human Kupffer cells (KC) were developed under static and orbital-induced mixing flow conditions.
View Article and Find Full Text PDF