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Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropology. In this study, we described the development of nine microsatellite loci from A. catechu. The number of alleles per locus ranged from 5 to 15. The expected and observed heterozygosities ranged from 0.71to 0.94 and from 0 to 0.88, respectively. All microsatellite loci, except for AC30, significantly deviated from Hardy-Weinberg equilibrium possibly due to artificially selected cultivation or the existence of excessive null alleles. No linkage disequilibrium was observed from pairwise comparisons of loci, except for AC06 and AC08.
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http://dx.doi.org/10.1111/j.1755-0998.2008.02504.x | DOI Listing |
PeerJ
September 2025
Guangxi Key Laboratory of Marine Environment Change and Disaster in Beibu Gulf, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou, Guangxi, China.
Background: The marine species , valued for its medicinal and commercial significance, faces critical research gaps due to a lack of molecular markers (notably simple sequence repeats (SSRs)) and insufficient genetic diversity data, hindering genetic studies and evidence-based breeding initiatives.
Methods: The software of Misa and Primer3 were adopted to detect SSRs and develop primer pairs, and then some primers of highly polymorphic loci in the genome were used to reveal the genetic diversity of along the Beibu Gulf in China.
Results: From the genome, 277,264 SSRs were detected an d 198,902 primers were designed.
BMC Plant Biol
September 2025
National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A & F University, Lin'an, Hangzhou, Zhejiang, 311300, China.
Background: The bamboo species Dendrocalamus × mutatus T.P.Yi & B.
View Article and Find Full Text PDFBMC Ecol Evol
September 2025
Laboratório de Marcadores Moleculares, Centro de Biotecnologia E Genética, Universidade Estadual de Santa Cruz, Ilhéus, Brazil.
Deforestation in the Atlantic Forest has reached critical levels, threatening multiple levels of biodiversity. In these deforested landscapes, conservation strategies could benefit from preserving agroforestry systems known as Cabruca, a traditional method of cultivating cocoa under a canopy of native trees. In this context, Cariniana legalis (Jequitibá-rosa), an endemic tree species of the Atlantic Forest listed as endangered, was selected to evaluate the role of cocoa agroforests (Cabrucas) and forest remnants in the genetic conservation of this species.
View Article and Find Full Text PDFNPJ Precis Oncol
August 2025
Department of Oncology, General Hospital of Northern Theater Command, Shenyang, Liaoning, China.
Microsatellite instability (MSI) serves as an important therapeutic and prognostic marker. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) have long been regarded as the "gold standard" for MSI detection. In recent years, next-generation sequencing (NGS)-based methods, offering expanded target coverage of microsatellite (MS) loci and improved analytical performance, have gained widespread acceptance.
View Article and Find Full Text PDFGenes (Basel)
August 2025
Department of Fish and Wildlife Sciences, University of Idaho, Moscow, ID 83844, USA.
Loss and fragmentation of habitat from agricultural conversion led to the near extirpation of the pygmy rabbit ( Merriam, 1891) population in the Columbia Basin (CB) of Washington, USA. Recovery efforts began in 2002 and included captive breeding, translocations from other regions for genetic rescue, and reintroduction into native habitat in three sites: Sagebrush Flat (SBF), Beezley Hills (BH), and Chester Butte (CHB). We used noninvasive and invasive genetic sampling to evaluate demographic and population genetic parameters on three translocated populations of pygmy rabbits over eight years (2011-2020).
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