98%
921
2 minutes
20
Objectives of this work are to investigate effects of pH and ionic strength (IS) on virus transport in saturated soil and to develop a quantitative relationship for these effects. A series of 50-cm column experiments with clean quartz sand under saturated conditions and with pH values of 5, 6, 7, 8, and IS values of 1, 10, and 20 mM were conducted. Bacteriophage PRD1 was used as a model virus. Applying a one-site kinetic model, attachment, detachment, and inactivation rate coefficients were determined from fitting breakthrough curves using the software package Hydrus-1D. Attachment rate coefficients increased with decreasing pH and increasing IS, in agreement with DLVO theory. Sticking efficiencies were calculated from the attachment rate coefficients and used to develop an empirical formula for sticking efficiency as a function of pH and IS. This relationship is applicable under unfavorable conditions for virus attachment. We compared sticking efficiencies predicted by the empirical formula with those from field and column experiments. Within the calibrated range of pH and IS, the predicted and observed sticking efficiencies are in reasonable agreement for bacteriophages PRD1 and MS2. However, the formula significantly overestimates sticking efficiencies for IS higher than 100 mM. In addition, it performs less well for viruses with different surface reactivity than PRD1 and MS2. Effects of pH and IS on detachment and inactivation rate coefficients were also investigated but the experimental results do not allow constraining these parameters with sufficient certainty.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1111/j.1745-6584.2010.00767.x | DOI Listing |
Vet Anim Sci
December 2025
Hunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, Changsha Medical University, Changsha 410219, China.
Muscovy duck reovirus (MDRV) and Novel duck reovirus (NDRV) are highly infectious diseases of waterfowl, causing significant harm to the global poultry industry. Early detection and diagnosis of NDRV and MDRV in clinical samples are crucial for effectively preventing and controlling these diseases. This study developed a duplex crystal digital PCR (dPCR) assay for the differential detection of NDRV and MDRV.
View Article and Find Full Text PDFACS Electrochem
September 2025
Liquid Sunlight Alliance, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Reaction rate coefficients for electron-transfer processes at the electrode-electrolyte interface are commonly estimated by using the Butler-Volmer equation, but their values are inaccurate beyond a few tenths of volts of overpotential. The Marcus-Hush-Chidsey (MHC) formalism yields correct asymptotic behavior of the rate coefficients vs applied overpotential but has complex dependencies on the redox system's intrinsic parameters, which can be difficult to model or measure. In this work, we bridge the two kinetics formalisms to estimate the reorganization energy, one of the important parameters for the MHC formalism, and investigate its dependence on other intrinsic parameters such as activation barriers, electronic coupling strength, and the density of states of the electrode surface.
View Article and Find Full Text PDFTemperature (Austin)
June 2025
Department of Mechanical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Sweating is a vital thermoregulatory mechanism in humans for maintaining thermal balance during exercise and exposure to hot environments. The development of models that predict sweat rate based on body temperature has been ongoing for over half a century. Here, we compared predicted water loss rates (WLR) from these models to actual observations collected during 780 participant-exposures in three independent laboratory-based experiments.
View Article and Find Full Text PDFUltrason Sonochem
September 2025
College of Science, Inner Mongolia University of Technology, Hohhot 010051, China.
In this study, the systematic investigation focused on how varying power levels of ultrasonic (US) pretreatment, when integrated with electrohydrodynamic (EHD) drying, influence the physicochemical properties of yam. Yam samples were subjected to ultrasonic pretreatment at 30 °C for 30 min using power levels of 0 W (Control), 150 W, 180 W, 210 W, 240 W, and 270 W, respectively, followed by drying in an EHD system. During the drying process, a range of metrics were measured, including moisture content, average drying rate, color change, as well as rehydration capacity.
View Article and Find Full Text PDFInt J Sports Physiol Perform
September 2025
Department of Kinesiology, The Pennsylvania State University, University Park, PA, USA.
Purpose: To assess the association between skeletal-muscle endurance performance and mitochondrial oxidative capacity of the hamstrings as respectively measured by biomechanical and physiological standards.
Methods: Nineteen (12 men and 7 women) healthy, young, recreationally active participants enrolled in our study. Participant characteristics comprised a mean and SD age of 21.