Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The Predictive Safety Testing Consortium's first regulatory submission to qualify kidney safety biomarkers revealed two deficiencies. To address the need for biomarkers that monitor recovery from agent-induced renal damage, we scored changes in the levels of urinary biomarkers in rats during recovery from renal injury induced by exposure to carbapenem A or gentamicin. All biomarkers responded to histologic tubular toxicities to varied degrees and with different kinetics. After a recovery period, all biomarkers returned to levels approaching those observed in uninjured animals. We next addressed the need for a serum biomarker that reflects general kidney function regardless of the exact site of renal injury. Our assay for serum cystatin C is more sensitive and specific than serum creatinine (SCr) or blood urea nitrogen (BUN) in monitoring generalized renal function after exposure of rats to eight nephrotoxicants and two hepatotoxicants. This sensitive serum biomarker will enable testing of renal function in animal studies that do not involve urine collection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/nbt.1627 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Advanced Three-Dimensional Cell Culture Models for Investigating Enterohaemorrhagic Pathogenesis and Shiga Toxin-Mediated Injury.
J Microbiol Biotechnol
September 2025
Environmental Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Enterohemorrhagic (EHEC), a pathotype within the Shiga toxin-producing (STEC) group, is a major etiological agent of severe gastrointestinal illness and life-threatening sequelae, including hemolytic uremic syndrome. Although insights into EHEC pathogenesis have been gained through traditional 2D cell culture systems and animal models, these platforms are limited in their ability to recapitulate human-specific physiological responses and tissue-level interactions. Recent progress in three-dimensional (3D) cell culture systems, such as spheroids, organoids, and organ-on-a-chip (OoC) technologies, has enabled more physiologically relevant models for investigating host-pathogen dynamics.
View Article and Find Full Text PDFQuantitative Susceptibility Mapping for Evaluating Renal Functional Injury in Chronic Kidney Disease Patients: A Pilot Study.
NMR Biomed
October 2025
Department of Radiology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
Chronic kidney disease (CKD) is an increasing global health problem, resulting in gradual loss of renal function and irreversible renal injury. The noninvasive detection, monitoring, and timely intervention of CKD might benefit the patients' prognosis. This study aims to assess renal functional injury in CKD patients by using magnetic resonance imaging (MRI) of quantitative susceptibility mapping (QSM).
View Article and Find Full Text PDFA novel treatment for diabetic nephropathy: Folate receptor-targeted delivery of TLR4 siRNA via functionalized PLGA nanoparticles in streptozotocin-induced diabetic murine models.
Nanomedicine
September 2025
The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, 730000, Gansu, People's Republic of China; Department of Nephrology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, 730030, Gansu, People's Republic of China; Key laboratory of nephropathy, The S
Diabetic kidney disease (DKD), a prominent microvascular complication of diabetes mellitus and the leading cause of end-stage renal disease (ESRD), was addressed through a novel nanotherapeutic approach. This study engineered folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles (FA-PLGA NPs) for the folate receptor (FR)-targeted delivery of Toll-like receptor 4 small interfering RNA (TLR4 siRNA) to treat diabetic nephropathy (DN). In a streptozotocin-induced DN murine model, administration of FA-PLGA NPs/TLR4 siRNA significantly mitigated renal injury compared to untreated DN controls.
View Article and Find Full Text PDFDetermination of the inter-observer and inter-scanner variability in the measurement of the renal resistive and renal pulsatility indices in dogs.
Res Vet Sci
September 2025
Small Animal Emergency and ICU Service, Complutense Veterinary Clinical Teaching Hospital, Complutense University, Avda Puerta del Hierro sn, Madrid 28040, Spain.
Renal Resistive Index (RRI) and Renal Pulsatility Index (RPI) are currently used in the diagnosis of ureteral obstruction, early diagnosis and follow-up of acute kidney injury, assessment of chronic kidney disease, and evaluation of transplanted kidneys. However, their inter-observer and inter-scanner variability has not been investigated in dogs, limiting the accuracy and clinical applicability of these indices. The objectives of this cross-sectional observational prospective study were to assess the inter-observer and inter-scanner variability of RRI and RPI and to determine whether operator experience influences measurement accuracy.
View Article and Find Full Text PDFEndothelial Dysfunction and Therapeutic Advances in Chronic Kidney Disease.
Diabetes Metab Res Rev
September 2025
Department of Nephrology, Daping Hospital, Army Medical University, Chongqing, China.
Chronic kidney disease (CKD) substantially increases cardiovascular risk, with endothelial dysfunction as its central pathological mechanism. This review summarises the molecular regulatory mechanisms underlying endothelial dysfunction in CKD and highlights recent advances in treatment strategies. The pathophysiology of endothelial injuries involves a complex network of multiple factors and mechanisms, including oxidative stress, inflammation, glycocalyx damage, ischaemia, hypoxia, cellular senescence and endothelial-mesenchymal transition (EndMT).
View Article and Find Full Text PDF