Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Vasopressin (VP) mRNA and the non-coding BC200 RNA are sorted to neuronal dendrites. Among proteins interacting specifically with both RNAs is the multifunctional poly(A)-binding protein (PABP) consisting of four RNA recognition motifs (RRMs) and a C-terminal auxiliary domain. The protein/RNA interaction studies presented here reveal that PABPs association with VP- and BC200 RNA is exclusively mediated by RRMs 3+4. Quantitative binding studies with PABP deletion mutants demonstrate preferential binding of RRMs 3+4 even to poly(A)-homopolymers, while RRMs 1+2 exhibit a lower affinity for those sequences. An optimal interaction with both poly(A)- and non-poly(A) sequences is only achieved by full-size PABP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.febslet.2004.09.054 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structured and disordered regions of Ataxin-2 contribute differently to the specificity and efficiency of mRNP granule formation.
PLoS Genet
May 2024
School of Biosciences and Bioengineering, Indian Institute of Technology, Mandi, India.
Ataxin-2 (ATXN2) is a gene implicated in spinocerebellar ataxia type II (SCA2), amyotrophic lateral sclerosis (ALS) and Parkinsonism. The encoded protein is a therapeutic target for ALS and related conditions. ATXN2 (or Atx2 in insects) can function in translational activation, translational repression, mRNA stability and in the assembly of mRNP-granules, a process mediated by intrinsically disordered regions (IDRs).
View Article and Find Full Text PDFThe polyA tail facilitates splicing of last introns with weak 3' splice sites via PABPN1.
EMBO Rep
October 2023
College of Life Sciences, TaiKang Center for Life and Medical Sciences, RNA Institute, Wuhan University, Wuhan, China.
The polyA tail of mRNAs is important for many aspects of RNA metabolism. However, whether and how it regulates pre-mRNA splicing is still unknown. Here, we report that the polyA tail acts as a splicing enhancer for the last intron via the nuclear polyA binding protein PABPN1 in HeLa cells.
View Article and Find Full Text PDFMAPKAP Kinase-2 phosphorylation of PABPC1 controls its interaction with 14-3-3 proteins after DNA damage: A combined kinase and protein array approach.
Front Mol Biosci
April 2023
David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, United States.
Article Synopsis
- 14-3-3 proteins are essential for managing cellular responses to stress and DNA damage, influencing processes like metabolism, cell cycle, migration, and apoptosis by binding to specific proteins after they're modified by kinases.
- Despite identifying over 200 proteins that interact with 14-3-3 through proteomic studies, the specific kinases involved in these interactions are often unknown.
- Researchers developed a method to pinpoint these kinase-specific interactions, discovering that the protein PABPC1 is a target for kinases Chk1 and MK2, with a specific site (Ser-470) crucial for its binding to 14-3-3; loss of this binding leads to increased cell growth and reduced cell death following DNA damage
Proteomes from AMPK-inhibited peripheral blood mononuclear cells suppress the progression of breast cancer and bone metastasis.
Theranostics
March 2023
Department of Biomedical Engineering, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USA.
During a developmental process, embryos employ varying tactics to remove unwanted cells. Using a procedure analogous to some of the embryonic cells, we generated a tumor-eliminating conditioned medium (CM) from AMPK-inhibited lymphocytes and monocytes in peripheral blood mononuclear cells (PBMCs). AMPK signaling was inhibited by the application of a pharmacological agent, Dorsomorphin, and the therapeutic effects of their conditioned medium (CM) were evaluated using cell cultures, breast cancer tissues, and a mouse model of mammary tumors and tumor-induced osteolysis.
View Article and Find Full Text PDFDiverse yeast antiviral systems prevent lethal pathogenesis caused by the L-A mycovirus.
Proc Natl Acad Sci U S A
March 2023
Department of Molecular Genetics, University of Toronto, Toronto, ON M5G 1M1, Canada.
Recent studies show that antiviral systems are remarkably conserved from bacteria to mammals, demonstrating that unique insights into these systems can be gained by studying microbial organisms. Unlike in bacteria, however, where phage infection can be lethal, no cytotoxic viral consequence is known in the budding yeast even though it is chronically infected with a double-stranded RNA mycovirus called L-A. This remains the case despite the previous identification of conserved antiviral systems that limit L-A replication.
View Article and Find Full Text PDF