Silencing Ditylenchus destructor cathepsin L-like cysteine protease has negative pleiotropic effect on nematode ontogenesis.

Ditylenchus destructor is a migratory plant-parasitic nematode that severely harms many agriculturally important crops. The control of this pest is difficult, thus efficient strategies for its management in agricultural production are urgently required. Cathepsin L-like cysteine protease (CPL) is one important protease that has been shown to participate in various physiological and pathological processes.

New Diterpene and Indole Alkaloid Analogs from the Streptomyces malaysiensis SCSIO 41397.

One new cyclooctatin-type diterpenoid, 15-hydroxycyclooctatin (1), and one new indole alkaloid, streptoprenylindole D (3), along with 9 known compounds, were isolated from the Streptomyces malaysiensis SCSIO 41397. Their structures were established on the basis of spectroscopic analysis, optical rotation, and by a comparison with data from the literature. All isolated compounds were evaluated for their antibacterial (MRSA), antitumor (22Rv1 and PC-3) and antiviral (HSV-1/2) activities.

Cyclopentenone-Containing Tetrahydroquinoline and Geldanamycin Alkaloids from as Potential Anti-Androgens against Prostate Cancer Cells.

Malaymycin (), a new cyclopentenone-containing tetrahydroquinoline alkaloid, and mccrearamycin E (), a geldanamycin analogue bearing a rare ring-contracted cyclopentenone moiety, and a -symmetric macrodiolide () were isolated from SCSIO41397. Their structures including absolute configurations were determined by detailed analyses of NMR and HRMS data and ECD calculations. The occurrence of mccrearamycin E () bearing a ring-contracted cyclopentenone is rare in the geldanamycin class.

Structurally diverse sesquiterpenoids and polyketides from a sponge-associated fungus Aspergillus sydowii SCSIO41301.

Five new compounds, including one bisabolane-type sesquiterpenoids, namely aspergillusene D (1), two new xanthones (2 and 3), and two new catecholderivatives (4 and 5), together with fourteen known compounds (6-19), were isolated and identified from the fungus Aspergillus sydowiiSCSIO 41,301 from the sponge Phakellia fusca. Their structures and absolute configurations were determined by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. Most of the isolated compounds (1-3, and 6-19) were evaluated for their antiviral, cytotoxic, and antibacterial activities.

Cytotoxic anthracycline and antibacterial tirandamycin analogues from a marine-derived Streptomyces sp. SCSIO 41399.

Aranciamycin K (1) and isotirandamycin B (2) were isolated from a marine-derived Streptomyces sp. SCSIO 41399, along with the previously reported four anthracycline derivatives (3-6), and two known tirandamycin derivatives (7 and 8). Their structures including absolute configurations were determined by extensive analysis of their spectroscopic analysis and ECD calculation method.

Soliseptide A, A Cyclic Hexapeptide Possessing Piperazic Acid Groups from Streptomyces solisilvae HNM30702.

Soliseptide A (1), a cyclic hexapeptide possessing piperazic acid groups, together with two known azalomycin derivatives (2 and 3) were isolated from Streptomyces solisilvae HNM30702. Their structures were determined through spectroscopic methods and single crystal X-ray diffraction analysis. Soliseptide A (1) possessed a cyclic hexapeptide core featured with two piperazic acid units rarely discovered in nature, and exhibited weak antibacterial and antiviral activities.

Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response.

Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from L.