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Article Abstract
Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from L., comprising three class A (, - and -) and two class B ( and -) isoforms. Expressions of all dioscorin genes gradually decreased in mother tubers during yam sprouting and regrowth. On the other hand, all dioscorin genes accumulated transcripts progressively with tuber development in new tubers, with being the most prominent isoform. In yam leaves, the expressions of 5 were up-regulated by the treatments of five phytohormones (gibberellic acid, salicylic acid, indole-3-acetic acid, abscisic acid, and ethylene), and three abiotic stresses (high-temperature, low-temperature and drought). To further elucidate the regulatory mechanisms of expressions, transgenic plants harboring the promoter-β-glucuronidase (GUS) fusion were generated. GUS staining showed that expressions of the promoter were detected mainly in the shoot apical meristem (SAM) and hypocotyls, and enhanced by the treatments of the five hormones, and the three abiotic stresses mentioned above. These results suggest diverse roles of in yam sprouting, regrowth, and tuberization, as well as in response to enviromental cues.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536067 | PMC |
| http://dx.doi.org/10.3390/ijms18071579 | DOI Listing |
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Article Synopsis
- Dioscorins, the main storage proteins in yam tubers, show biochemical and immunomodulatory functions, making them interesting for biomedical research.
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