Distribution for platelet antibody in patients with immune-mediated platelet transfusion refractoriness.

Objectives: HLA Class I, HPA (Human platelet antigen), CD36 allo/isoantibodies, and platelet glycoprotein autoantibodies are the primary causes of immune-mediated platelet transfusion refractoriness (iPTR). Detecting these antibodies and selecting antigen-negative platelets for transfusion effectively manages iPTR, but large-scale data on platelet antibody distribution in the Chinese population are scarce.

Methods: From Jan 2021 to Dec 2023, 2073 patients with suspected iPTR underwent platelet cross-matching via solid-phase red blood cell adherence.

Identification of the Novel HLA-C*03:641 Allele in a Chinese Cord Blood Donor.

HLA-C*03:641 shows one nucleotide substitution when compared to HLA-C*03:02:02:01.

Identification of the Novel HLA-DRB1*08:03:16 Allele by Next-Generation Sequencing.

HLA-DRB1*08:03:16 differs from HLA-DRB1*08:03:02:01 by one nucleotide substitution at position 528 G>T.

Characterisation of the Novel HLA-DQB1*04:01:07 Allele by Next-Generation Sequencing.

HLA-DQB1*04:01:07 differs from HLA-DQB1*04:01:01:01 by one nucleotide substitution at position 735 in exon 4.

The Novel Allele HLA-A*26:01:80 Was Identified in a Chinese Individual.

The sequence of HLA-A*26:01:80 differs from HLA-A*26:01:01 by one single nucleotide substitution.

Constructing a potential HLA haplo-homozygous induced pluripotent stem cell haplobank using data from an umbilical cord blood bank.

Background: Induced pluripotent stem cells (iPSCs) can differentiate into any type of cell and have potential uses in regenerative medicine for the treatment of many diseases. However, reducing immune rejection is a key problem in the application of iPSCs that can be solved by the development of haplobanks containing specially selected iPSC lines.

Methods: To study the feasibility of constructing an HLA (human leukocyte antigen)-matched induced pluripotent stem cell haplobank in China, 5421 umbilical cord blood samples were randomly collected from the Umbilical Cord Blood Bank of Zhejiang Province, China.

[Establishment and Application of Efficient Gene Editing Method for Classical HLA-I Molecules].

Objective: To establish an efficient gene editing method of HLA-I gene to prepare HLA-I universal hematopoietic stem cells.

Methods: The easyedit small guide RNA(sgRNA) was designed according to the sequences of β2 microglobulin gene and synthesized by GenScript company. RNP complexes were formed by NLS-Cas9-NLS nuclease and Easyedit sgRNA according to different molar ratios (1∶1~1∶4).

Establishment and Application of a Multiplex PCR NGS Method for the Genotyping of HLA-Class I and HPA.

Selecting compatible HLA-Class I and/or HPA platelets based on genotyping could alleviate immune platelet transfusion refractoriness (PTR). A fast and reliable method of HLA-Class I and HPA genotyping is necessary to construct a platelet donor bank with known HLA-Class I and HPA genotypes. Ten pairs of specific primers for HLA-A, HLA-B, HLA-C, HPA-1 through HPA-6w, HPA-15 and HPA-21w were designed.

Identification of the novel HLA-DRB1*11:298 allele in a Chinese cord blood donor.

HLA-DRB1*11:298 shows one single nucleotide substitution at position 397 T>G compared with HLA-DRB1*11:01:01:01.

The HLA-DRB1*12:92 and HLA-DRB1*12:101 alleles were identified by next-generation sequencing.

Compared with HLA-DRB1*12:02, the alleles HLA-DRB1*12:92 and HLA-DRB1*12:101 each show one nucleotide substitution respectively.

Identification of the novel HLA-A*29:171 allele by next-generation sequencing.

HLA-A*29:171 differs from HLA-A*29:01:01:01 by one nucleotide substitution at position 257T>G in exon 2.

Identification of the novel HLA-A*03:453 allele in a Chinese blood donor.

HLA-A*03:453 differs from HLA-A*03:02:01:01 by one single nucleotide substitution at position 376 G > A.

DHOK Exerts Anti-Cancer Effect Through Autophagy Inhibition in Colorectal Cancer.

DHOK (14,15β-dihydroxyklaineanone) is a novel diterpene isolated from roots of , a traditional herb widely applied in Southeast Asia. It is reported that DHOK has cytotoxic effect on cancer cells, but its anti-cancer mechanism has still been not clear. In our study, we first observed that DHOK inhibits cell proliferation of colorectal cancer cells in a time- and dose-dependent manner.

Correction: Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures.

[This corrects the article DOI: 10.1039/C6RA27334C.].

Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells.

Abnormal angiogenesis is associated with intraocular diseases such as proliferative diabetic retinopathy and neovascular age-related macular degeneration, and current therapies for these eye diseases are not satisfactory. The purpose of this study was to determine whether capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, can inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis signaling events and cellular responses in primary human retinal microvascular endothelial cells (HRECs). Our study revealed that the capilliposide B IC for HRECs was 8.

A novel Diels-Alder adduct of mulberry leaves exerts anticancer effect through autophagy-mediated cell death.

Guangsangon E (GSE) is a novel Diels-Alder adduct isolated from leaves of Morus alba L, a traditional Chinese medicine widely applied in respiratory diseases. It is reported that GSE has cytotoxic effect on cancer cells. In our research, we investigated its anticancer effect on respiratory cancer and revealed that GSE induces autophagy and apoptosis in lung and nasopharyngeal cancer cells.

A Novel Benzofuran Derivative Moracin N Induces Autophagy and Apoptosis Through ROS Generation in Lung Cancer.

Introduction: The leaves of is a traditional Chinese medicine widely applied in lung diseases. Moracin N (MAN), a secondary metabolite extracted form the leaves of L, is a potent anticancer agent. But its molecular mechanism remains unveiled.

Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability.

The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes' preparation and optimization.