Role of BABY BOOM Transcription Factor in Promoting Somatic Embryogenesis and Genetic Transformation in a Woody Magnoliid Liriodendron.

Somatic embryogenesis (SE) is a powerful biotechnological tool widely utilized for large-scale propagation and genetic transformation. Morphogenic genes like BABY BOOM (BBM) and WUSCHEL (WUS) play crucial roles in SE and are extensively applied to improve SE-based genetic transformation. However, the transcriptome profiling and key regulatory factors of SE in the woody magnoliid Liriodendron hybrid remain unclear.

Highly Efficient Homozygous CRISPR/Cas9 Gene Editing Based on Single-Cell-Originated Somatic Embryogenesis in .

The clustered, regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system is the most widely used gene-editing tool to date. However, its application in the genetic improvement of forestry trees has been largely limited. Here, we first established a highly efficient multi-target editing system in the magnoliid woody plant .

Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis in hybrids.

The precise expression of transcription factors (TFs) is crucial for plant growth and development, especially during somatic embryogenesis. However, conventional overexpression approaches, commonly used for functional genetics, can lead to deleterious effects. Therefore, it is imperative to ensure that TFs are expressed in a controlled and timely manner when aiming to enhance the efficiency of somatic embryogenesis.

The Role of Gene Family in Abiotic Stress Response.

The DOF (DNA-binding with one finger) transcription factors are exclusive to plants and play crucial roles in plant growth, development, and environmental adaptation. Although extensive research has been conducted on the gene family in , , and , investigations concerning the role of this gene family in remain unreported, leaving its biological function largely unknown. In this study, we performed a comprehensive genome-wide identification of the gene family based on the genome, resulting in the discovery of a total of 17 gene members.

Overexpression of Hybrid in Leads to Excessive Chlorophyll Synthesis and Improved Growth.

Chloroplasts is the site for photosynthesis, which is the main primary source of energy for plants. Golden2-like (GLK) is a key transcription factor that regulates chloroplast development and chlorophyll synthesis. However, most studies on genes are performed in crops and model plants with less attention to woody plants.

Genome-Wide Analysis of the Gene Family under Abiotic Stress and Characterization of the Gene.

Heat shock factors (Hsfs) play a crucial role in plant defense processes. However, the distribution and functional characteristics of Hsf genes in the relict plant are still unclear. In this study, a total of 19 were identified and divided into three separate subgroups, comprising 10 , 7 , and 2 genes, respectively, based on their phylogenetic tree and the presence/absence of conserved protein domains.

Genomic survey and expression analysis of LcARFs reveal multiple functions to somatic embryogenesis in Liriodendron.

Background: Auxin response factors (ARFs) are critical transcription factors that mediate the auxin signaling pathway and are essential for regulating plant growth. However, there is a lack of understanding regarding the ARF gene family in Liriodendron chinense, a vital species in landscaping and economics. Thus, further research is needed to explore the roles of ARFs in L.

Physiological and transcriptomic analysis revealed that the accumulation of reactive oxygen species caused the low temperature sensitivity of Liriodendron × sinoamericanum.

Liriodendron × sinoamericanum is widely cultivated in southern China as an excellent wood and garden ornamental trees. However, its intolerance to low temperature limits its application to high latitudes. Understanding the molecular mechanism of low temperature sensitivity of Liriodendron × sinoamericanum is very important for its further application.

The Identification and Expression Analysis of the Gene Family.

The gene family is one of the largest gene families in plants, and it plays a crucial role in regulating plant development, reproduction, cellular protein degradation, and response to biotic and abiotic stresses. Despite their significance, a comprehensive analysis of the gene family in and other magnoliaceae species has not been reported. In this study, we report for the first time the identification of 144 full-length genes in .

HO Significantly Affects × Somatic Embryogenesis.

Larch is widely distributed throughout the world and is an important species for timber supply and the extraction of industrial raw materials. In recent years, the hybrid breeding of and has shown obvious heterosis in quick-growth, stress resistance and wood properties. However, its growth and development cycle is too long to meet general production needs.

Genome-wide analyses of calmodulin and calmodulin-like proteins in the halophyte Nitraria sibirica reveal their involvement in response to salinity, drought and cold stress.

The calmodulin (CaM) and calmodulin-like (CML) proteins are major calcium sensors that play a critical role in environmental stimulus response in plants. Nevertheless, the CaM/CML proteins from the specific plants with extreme tolerance to abiotic stresses remained so far uncharacterized. In this study, 66 candidate proteins (three NsCaMs and sixty-three NsCMLs) were identified from the halophyte Nitraria sibirica, which can withstand an extreme salinity.

Phytosulfokine contributes to suspension culture of Cunninghamia lanceolata through its impact on redox homeostasis.

Background: Suspension culture is widely used in the establishment of efficient plant regeneration systems, as well as in the mass production of plant secondary metabolites. However, the establishment of a suspension culture system of Cunninghamia lanceolata is genotype-dependent given that proembryogenic masses (PEMs) are prone to browning during this process in recalcitrant genotypes. Previously, we reported that the plant peptide hormone phytosulfokine (PSK) can tremendously decrease the hydrogen peroxide (HO) level and help to initiate somatic embryogenesis (SE) in recalcitrant C.

Genome-wide analysis of the GRAS gene family in reveals the putative function in abiotic stress and plant development.

Introduction: genes encode plant-specific transcription factors that play essential roles in plant growth and development. However, the members and the function of the gene family have not been reported in . , a tree species in the family that produces excellent timber for daily life and industry.

Genome-wide identification of the NAC gene family and its functional analysis in Liriodendron.

As one of the largest plant specific transcription factor families, NAC family members play an important role in plant growth, development and stress resistance. To investigate the function of NAC transcription factors during abiotic stress, as well as during somatic embryogenesis, we identified and characterized the NAC gene family in Liriodendron chinense. We found that most LcNAC members contain more than three exons, with a relatively conserved gene and motif structure, especially at the N-terminus.

Family Genes from Positively Respond to Multiple Stresses.

The phytohormone abscisic acid (ABA) plays important roles in response to abiotic and biotic stresses in plants. Pyrabactin resistance 1-like (PYR/PYL) proteins are well-known as ABA receptors, which are responsible for ABA signal transduction. Nevertheless, the characteristics of genes from , an endangered timber tree, remain unclear in coping with various stresses.

Characterization of the Pentatricopeptide Repeat (PPR) Gene Family and Its Role in Osmotic Stress Response.

The () superfamily is a large gene family in plants that regulates organelle RNA metabolism, which is important for plant growth and development. However, a genome-wide analysis of the gene family and its response to abiotic stress has not been reported for the relict woody plant . In this paper, we identified 650 genes from the genome.

Expression dynamics of WOX homeodomain transcription factors during somatic embryogenesis in hybrids.

The relict woody plant genus contains two endangered species, and . Understanding the molecular mechanisms involved in early embryo development is important for horticultural and ecological research, particularly for the development of improved somatic embryogenesis systems. However, the specific molecular processes underlying embryogenesis in these species remain largely unexplored.

Genome-Wide Identification and Abiotic-Stress-Responsive Expression of Gene Family in .

is a tree species of the family, an ancient relict plant mainly used for landscaping and timber production due to its excellent material properties and ornamental value. The cytokinin oxidase/dehydrogenase (CKX) enzyme regulates cytokinin levels and plays an important role in plant growth, development, and resistance. However, too-high or too-low temperatures or soil drought can limit the growth of , representing a key issue for research.

The Glutathione Peroxidase Gene Family in : Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions.

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain HO homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the gene family and its responses to environmental stresses, especially salt stress, in , which is a shrub that can survive in saline environments, has not yet been reported. Here, we first report the genome-wide analysis of the gene family in , leading to a total of seven genes that are distributed on six of the twelve chromosomes.

AT-Hook Transcription Factors Show Functions in under Drought Stress and Somatic Embryogenesis.

AT-hook motif nuclear localized (AHL) is a transcription factor that can directly induce plant somatic embryogenesis without adding exogenous hormones. One of its functional domains, the AT-hook motif, has a chromatin-modifying function and participates in various cellular processes, including DNA replication and repair and gene transcription leading to cell growth. (Hemsl.

Genome-Wide Identification and Expression Analysis of Gene Family in .

Terpenoids play a key role in plant growth and development, supporting resistance regulation and terpene synthase (TPS), which is the last link in the synthesis process of terpenoids. commonly called the Chinese tulip tree, is a rare and endangered tree species of the family Magnoliaceae. However, the genome-wide identification of the gene family and its transcriptional responses to development and abiotic stress are still unclear.

miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana.

Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro-RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive.