A dansyl-quinolinium-based ratiometric fluorescent probe for detecting HSO/SO in living cells.

Background: Sulfur dioxide (SO) is recognized as a major atmospheric pollutant and its excessive emissions can pose a great threat to the environment, flora and fauna, and human health. Long-term exposure to excessive SO can cause chronic poisoning, leading to neurological disorders and cardiovascular diseases. However, there are two sides to everything.

A novel FRET-based fluorescent probe capable of simultaneously imaging lipid droplets and the endoplasmic reticulum with two distinct fluorescence signals in HeLa cells.

Inter-organellar interactions play indispensable roles in regulating cellular homeostasis, necessitating advanced methodologies for their simultaneous and discriminative visualization. Fluorescent probes, prized for their sensitivity and spatiotemporal resolution, are pivotal tools for elucidating organelle dynamics in live-cell studies. However, current technologies remain limited in achieving robust dual-color imaging of multiple organelles with minimal crosstalk.

Lysosomal-targeted fluorescent probe for detecting SO derivatives in human umbilical vein endothelial cells.

A fluorescent probe (NBC), constructed by benzothiazole-coumarin and naphthalimide derivatives, was developed for the detection of SO derivatives using the FRET (Förster Resonance Energy Transfer) strategy. NBC presented large Stokes shift (180 nm), fast response (2 min), high sensitivity (LOD: 45 nM) and an excellent linear relationship in response to SO derivatives. Moreover, NBC has been successfully applied to detect SO derivatives in food samples and living cells.

Activating Esterase D by PFD5 exerts antiviral effect through inhibiting glutathionization of LAMP1 during Senecavirus A infection.

Seneca virus A (SVA) is a newly discovered small nucleic acid virus, which can cause swine blister disease (PVD). Currently, there is no drug or vaccine. Studies have shown that SVA relies on the endolysosomal pathway to accomplish intracellular transport and release, and can disrupt lysosomal homeostasis, but its specific mechanism has not been revealed.

Two "turn on" fluorescence probes based on nitroso recognition group for detecting hydrogen sulfide.

Hydrogen sulfide is a vital signaling molecule which holds a pivotal position in numerous biological functions. In this research, two novel "OFF-ON" fluorescence probes named YNO and TNO were designed based on the nitroso recognition group to detect HS. Both YNO and TNO performed outstanding response rate and linear relationship between the fluorescence intensity and the concentration of HS.

Novel fluorescence probe for ClO in living cells: Based on FRET mechanism.

Hypochlorous acid (HClO) as a kind of reactive oxygen species (ROS) plays a vital role in many biological processes. Organic fluorescence probes have attracted great interests for the detection of HClO, due to their relatively high selectivity and sensitivity, satisfactory spatiotemporal resolution and good biocompatibility. Constructing fluorescence probes to detect HClO with advantages of large Stokes shift, wide emission gap, near infrared emission and good water solubility is still challenging.

A near-infrared fluorescent probe for simultaneous detection of pH and viscosity.

In this work, we developed a ratiometric fluorescent probe (NT) based on ICT framework in near-infrared (NIR) which could detect pH and viscosity simultaneously. Long emission wavelength in NIR could protect the probe from interference of background fluorescence and improve the accuracy of the test. Due to the presence of thiazole-salt, the probe possessed good water solubility and could respond immediately to pH in water system.

A highly sensitive ratiometric fluorescent probe for detecting HSO/SO and viscosity change based on FRET/TICT mechanism.

Background: Sulfur dioxide (SO) is a significant gas signaling molecule in organisms, and viscosity is a crucial parameter of the cellular microenvironment. They are both involved in regulating many physiological processes in the human body. However, abnormalities in SO and viscosity levels are associated with various diseases, such as cardiovascular disease, lung cancer, respiratory diseases, neurological disorders, diabetes and Alzheimer's disease.

A near-infrared fluorescent probe based FRET for ratiometric sensing of HO and viscosity in live cells.

Hydrogen peroxide (HO) and viscosity play vital roles in the cellular environment as signaling molecule and microenvironment parameter, respectively, and are associated with many physiological and pathological processes in biological systems. We developed a near-infrared fluorescent probe, CQ, which performed colorimetric and ratiometric detection of HO and viscosity based on the FRET mechanism, and was capable of monitoring changes in viscosity and HO levels simultaneously through two different channels. Based on the specific reaction of HO with borate ester, CQ exhibited a significant ratiometric response to HO with a large Stokes shift of 221 nm, a detection limit of 0.

NBD-based colorimetric and ratiometric fluorescent probe in NIR for bisulfite.

This work presented a FRET-ICT based fluorescent probe (named NTC) composed of coumarin-benzothiazole as the acceptor and 4-nitrobenzo[c][1,2,5] oxadiazole (NBD) as the donor for the detection of SO derivatives in NIR. Probe NTC possessed superior performance including selectivity, quickly response toward SO/HSO and high energy transfer efficiency (94 %). The test strips provided a simple and effective tool in detecting the presence of bisulfite.

A ratiometric fluorescent probe based on the FRET platform for the detection of sulfur dioxide derivatives and viscosity.

Background: Sulfur dioxide (SO) is a common gaseous pollutant that significantly threatens environmental pollution and human health. Meanwhile, viscosity is an essential parameter of the intracellular microenvironment, manipulating many physiological roles such as nutrient transport, metabolism, signaling regulation and apoptosis. Currently, most of the fluorescent probes used for detecting SO derivatives and viscosity are single-emission probes or probes based on the ICT mechanism, which suffer from short emission wavelengths, small Stokes shifts or susceptibility to environmental background.

An efficient dual-function fluorescent probe for sulfites and sulfides and its imaging application in cells.

As gas signaling molecules in organisms, SO derivatives and HS play crucial regulating roles in a series of physiological processes. Therefore, developing an assay that can accurately monitor the concentration of SO derivatives and HS in cells is extremely important for the research and treatment of related illnesses. A bifunctional probe SN-F based on FRET mechanism for SO derivatives and HS was designed.

A benzothiazole-salt-based fluorescent probe for precise monitoring the changes of pH and viscosity.

This work presented a novel ratiometric fluorescent probe (NBO) based on benzothiazole dye, which could monitor the pH fluctuations with high sensitivity via the intramolecular charge transfer (ICT) process. NBO was developed with a good linear response in the pH range of 5.75-7.

FRET-based fluorescent probe with favorable water solubility for simultaneous detection of SO derivatives and viscosity.

The intracellular viscosity is an important parameter of the microenvironment and SO is a vital gas signal molecule. At present, some dual-response fluorescence probes for simultaneous measurements of viscosity and SO derivatives (HSO/SO) possessed poor water solubility. In this work, we developed a water-soluble fluorescence probe CIJ (0.

Esterase D interacts with metallothionein 2A and inhibits the migration of A549 lung cancer cells in vitro.

Esterase D (ESD) is a nonspecific esterase widely distributed in various organisms. ESD plays an important role in regulating cholesterol efflux, inhibiting viral replication and lung cancer growth. MT2A (metallothionein 2A) is the most important isoform of metallothionein (MTs) in human and high expression of MT2A in tumors represents poor prognosis and metastatic behavior.

Dual-response and lysosome-targeted fluorescent probe for viscosity and sulfur dioxide derivatives.

Viscosity and sulfur dioxide levels are important factors to evaluate the changes of cell micro-environment because a series of diseases usually occur when they are abnormal. At present, dual-response probes that can detect both viscosity and sulfur dioxide are rare. Therefore, we developed a novel fluorescent probe CBN for simultaneous detection of sulfur dioxide and viscosity.

Esterase D stabilizes FKBP25 to suppress mTORC1.

Background: Esterase D (ESD) is a nonspecific esterase that detoxifies formaldehyde. Many reports have stated that ESD activity is associated with a variety of physiological and pathological processes. However, the detailed signaling pathway of ESD remains poorly understood.

Two simple but effective turn-on benzothiazole-based fluorescent probes for detecting hydrogen sulfide in real water samples and HeLa cells.

Two simple turn-on fluorescent probes, containing a benzothiazole and the 2,4-dinitrobenzenesulfonyl group, were designed for detecting HS. Two probes exhibited good selectivity and high sensitivity, which were applied to detect the HS in real water samples. Probe P2 with a positive charge had better solubility than probe P1 in water; therefore, probe P2 was successfully applied to detect both the endogenous and exogenous HS in lysosomes of living HeLa cells.

The Discovery and Biosynthesis of Nicotinic Myxochelins from an sp. SDU34.

Myxobacteria are a prolific source of structurally diverse natural products, and one of the best-studied myxobacterial products is the siderophore myxochelin. Herein, we report two new compounds, myxochelins N () and O (), that are nicotinic paralogs of myxochelin A, from the terrestrial myxobacterium sp. SDU34; is functionalized with a rare 2-oxazolidinone.

HS probe CPC inhibits autophagy and promotes apoptosis by inhibiting glutathionylation of Keap1 at Cys434.

HS is actual an endogenous signaling gas molecule and involved in a range of cell physiological processes. However, the mechanism of endogenous HS regulating autophagy and apoptosis has not been thoroughly investigated. Here, we try to address this issue by using a HS probe, (E)-2-(4-(4-(7-(diethylamino)-2-oxo-2H-chromene-3-carbonyl)-piperazin-1-yl)-styryl)-1, 3, 3-trimethyl-3H-indol-1-ium iodide (CPC), which could react with endogenous HS.

A synergetic FRET/ICT platform-based fluorescence probe for ratiometric imaging of bisulfite in lipid droplets.

A deep-red emission and lipid droplets-targeted fluorescence probe (named ZFPy) for effective bioimaging of bisulfite was developed from flavone moiety and benzoindole derivative based on intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET) platform. ZFPy displayed promising fluorescence parameters including bright deep red fluorescence (615 nm), large Stokes shift (205 nm), extended emission window gap (140 nm), high absolute fluorescence quantum yield (4.1%) and stable emission signal output.

Discovery of new fluorescent thiazole-pyrazoline derivatives as autophagy inducers by inhibiting mTOR activity in A549 human lung cancer cells.

A series of fluorescent thiazole-pyrazoline derivatives was synthesized and their structures were characterized by H NMR, C NMR, and HRMS. Biological evaluation demonstrated that these compounds could effectively inhibit the growth of human non-small cell lung cancer (NSCLC) A549 cells in a dose- and time-dependent manner in vitro and inhibit tumor growth in vivo. The structure-activity relationship (SAR) of the compounds was analyzed.

A novel mitochondria-targeted ratiometric fluorescent probe for endogenous sulfur dioxide derivatives as a cancer-detecting tool.

A new mitochondria-targeted fluorescent probe RBC, constructed using a coumarin moiety which was selected as the donor and a benzothiazole derivative as the acceptor, for SO derivatives (HSO/SO) was presented. The probe designed on a new FRET platform showed high selectivity and a low detection limit. Importantly, the probe could respond to HSO/SO within 35 s.

Discovery of a fluorescigenic pyrazoline derivative targeting ubiquitin.

Despite significant process in ubiquitin modification by using traditional genetic methods, chemical small molecules that directly target and modify ubiquitin are little reported. Here, we find that a fluorescigenic pyrazoline derivative (FPD5) could do so effectively. Molecule docking revealed that lysine 11 of ubiquitin was the key contact residue.