"Inner swelling-outer restraint" strategy to fabricate poly (vinyl alcohol)/MoS-poly (vinyl alcohol)/sodium carboxymethyl cellulose Janus hydrogel for pressure-bearing cartilage replacement.

Due to the poor regeneration ability of cartilage tissue, the design and fabrication of permanent hydrogel cartilage scaffolds with mechanical properties matching is still an urgent challenge. In this study, we propose an "inner swelling-outer restraint" strategy to construct Janus hydrogel for pressure-bearing cartilage replacement, which is inspired by the "Lamina-splendens" structure of cartilage. As a proof of concept, the poly(vinyl alcohol)/carboxymethyl cellulose sodium (PVA/CMCNa) layer is designed to capture more fluid by introducing negatively charged aggregates, while the macromolecular conformation of the PVA/MoS layer can be densified through wet annealing, thereby increasing the liquid permeation resistance of the PVA/CMCNa layer.

[Stimulation mechanism of osteoblast proliferation and differentiation by Duzhong Decoction-containing serum through L-VGCCs].

This paper aimed to explore the effects of Duzhong Decoction(DZD)-containing serum on the proliferation and osteoblast differentiation of MC3T3-E1 cells through L-type voltage-gated calcium channels(L-VGCCs). L-VGCCs inhibitors, nifedipine and verapamil, were used to block L-VGCCs in osteoblasts. MC3T3-E1 cells were divided into a control group, a low-dose DZD-containing serum(L-DZD) group, a medium-dose DZD-containing serum(M-DZD) group, a high-dose DZD-containing serum(H-DZD) group, a nifedipine group, a H-DZD + nifedipine group, verapamil group, and a H-DZD + verapamil group.

Anti-PD-1 synergizes with RFA to suppress abscopal tumors and induce durable memory against recurrence in HCC.

Background And Aims: Radiofrequency ablation (RFA) is the first-line treatment for early-stage hepatocellular carcinoma (HCC). However, recurrence after curative RFA remains a significant challenge for HCC patients. Although RFA induces an immune response, the anti-tumor effect is often limited by the immunosuppressive tumor microenvironment.

Optimization of coagulation process and its influence on membrane fouling for Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor (AOA-MBR).

As part of sewage treatment, coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic (AOA) biological process. The importance in investigating the influence of coagulation on Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor (AOA-MBR) should be emphasized. In this study, systematic optimization of coagulation parameters for greater pollutant removal was conducted in terms of coagulant dosage, coagulation residence time and stirring hydraulic conditions.

ADP-Based Orbit Tracking Control for Deep Space Probe Flying Around Unknown Asteroid.

This article investigates the orbit tracking control problem for the deep space probe flying around an unknown asteroid under completely unknown dynamics. First, an orbit tracking control model for the relative motion of the probe to the asteroid is established. Then, a model-based controller is designed for the optimal tracking control problem and the asymptotic stability of the closed-loop system is proved.

Plant developmental stage drives the assembly and functional adaptability of endophytic microbial communities.

Introduction: The seeds of represent a unique category of herbaceous seeds that arise from triploid apomixis. They necessitate an exceptionally protracted maturation phase of 8 months, followed by a dormancy period of 4 months, before they can germinate and give rise to fully formed new plants. Currently, the connection between endophytic microbial communities in seeds and the host plant's development is largely unexplored.

Simulation of Aging and Bonding Properties of the Matrix/Filler Interface in Particle-Reinforced Composites.

To investigate the microscopic mechanism of aging-induced "dewetting" at the matrix/filler interface in Nitrate Ester Plasticized Polyether (NEPE) propellant, this study decoupled the aging process into two factors: crosslinking density evolution and nitrate ester decomposition. Molecular dynamics (MD) simulations were employed to construct all-component matrix models and matrix/filler interface models with varying aging extents. Key parameters including crosslinking density, mechanical properties, free volume fraction, diffusion coefficients of the matrix, as well as interfacial binding energy and radial distribution function (RDF) were calculated to analyze the effects of both aging factors on "debonding".

Interactive Explainable Deep Learning Model for Hepatocellular Carcinoma Diagnosis at Gadoxetic Acid-enhanced MRI: A Retrospective, Multicenter, Diagnostic Study.

Purpose To develop an artificial intelligence (AI) model based on gadoxetic acid-enhanced MRI to assist radiologists in hepatocellular carcinoma (HCC) diagnosis. Materials and Methods This retrospective study included patients with focal liver lesions (FLLs) who underwent gadoxetic acid-enhanced MRI between January 2015 and December 2021. All hepatic malignancies were diagnosed pathologically, whereas benign lesions were confirmed with pathologic findings or imaging follow-up.

Genome-wide identification of heat shock protein 90 family in Larimichthys crocea and expression analysis in response to thermal stress and Vibrio parahaemolyticus infection.

Members of the heat shock protein 90 family (HSP90s) are evolutionarily conserved and play crucial roles in protein transport, immune regulation and antigen presentation. In this study, five hsp90s were identified from the genome of large yellow croaker (Larimichthys crocea) and analyzed using bioinformatics. All five identified hsp90s encode proteins with HATPase_c and HSP90 domains, and are mainly localized in the cytoplasm, mitochondria and endoplasmic reticulum.

Changes in the assembly and functional adaptation of endophytic microbial communities in Amorphophallus species with different levels of resistance to necrotrophic bacterial pathogen stress.

Pcc is one of the key pathogenic factors responsible for destructive soft rot in konjac. To date, the assembly and functional adaptation of the plant endophytic microbiome under Pcc stress remain poorly understood. Here, we found that Pcc stress leads to rapid reorganization of the endogenous microbiome in multiple organs of both susceptible and resistant konjac plants.

Screening and preliminary analysis of antimicrobial peptide genes in Octopussinensis.

Antimicrobial peptides (AMPs) are small molecular peptides that widely exist in organisms to resist external microbial invasion and play a crucial role in the host's immune defense system. Owing to their functions of efficient broad-spectrum killing of pathogenic microorganisms, immune enhancement, and intestinal health improvement, they have emerged as a focal point in research on the immune defense of aquatic animals in recent years. In this study, a total of 105 putative AMP-derived genes from the genome were screened, and seven candidate AMPs were finally identified by analyzing the differential expression results of the hepatopancreas and the white body transcriptomes combined with machine learning algorithms.

Construction of Cells-Membrane-Cells Living Complexes for Cartilage Repair by Enhancing the Structural Stability of Fibrous Membranes.

3D cartilage tissue engineering scaffolds with stable structures are crucial for promoting cartilage tissue growth and repair. However, limited research attention is given to the effects of 3D cells-membrane-cells sandwich-like living complexes with enhanced structural stability for cartilage repair. In this study, silk fibroin/graphene oxide@kartogenin (SF/GO@KGN) fibrous membranes with improved structural stability are developed through the regulation of the crystallinity, and living complexes are constructed for cartilage repair using rat bone marrow stromal cells (rBMSCs) and the SF/GO@KGN fibrous membranes.

Synergistic healing of diabetic wounds through photothermal and peroxidase-like activity of heterogeneous BiS/Au nanoparticles.

Bacterial resistance and biofilm formation around diabetic wounds are major challenges that make the wounds difficult to heal. It is crucial for diabetic wound healing to improve the microenvironment around the wounds. In this study, a novel strategy for diabetic wound healing is developed by combining the peroxidase (POD)-like enzyme activity and photothermal therapy (PTT) to protect against bacterial infections around the wounds.

Genome-Wide Identification and Expression Pattern of the Gene Family in .

Background: The NAC transcription factor family of genes is one of the largest families of transcription factors in plants, playing important functions in plant growth and development, response to adversity stress, disease resistance, and hormone signaling. In this study, we identified the number of members of the NAC () gene family and conducted a comprehensive analysis of their physicochemical characteristics, chromosomal location, evolutionary features, and expression patterns both in different parts of the plant at different growth stages and in response to infection by .

Methods: The gene family in was identified using Hidden Markov Model (HMMER) and National Center of Biotechnology Information Conserved Domain Database (NCBI CDD), and their physicochemical properties were analyzed with Perl scripts.

Sorbicitrinols A and B, Two Sorbicillinoids with Unprecedented Skeletons Uncovered from Sponge-Derived Fungus sp. S4I4-3 by a Molecular Networking Strategy.

Sorbicitrinol A () and its biosynthetic precursor sorbicitrinol B (), two novel sorbicillinoids with unprecedented skeletons, were isolated from the sponge-derived fungus sp. by molecular networking. Their structures were identified by spectroscopic analyses and quantum chemistry.

Periplakin Attenuates Liver Fibrosis via Reprogramming CD44 Cells into CD44 Liver Progenitor Cells.

Background & Aims: Liver progenitor cells (LPCs) contribute significantly to the restoration of injured liver parenchyma and promote liver regeneration, thereby ameliorating liver fibrosis. However, the mechanism of the derivation of LPCs remains poorly understood.

Methods: We first examined the expression of periplakin (PPL) in patients and mouse models with liver fibrosis.

Melodinines J Induces Apoptosis in Temozolomide-Resistant Glioma Cells by Disrupting TMX1-Dependent Homeostasis of Endoplasmic Reticulum-Mitochondria-Associated Membrane Contacts.

Glioma is recognized as one of the most lethal and aggressive brain tumors. Although the standard-of-care treatment for glioblastoma (GBM) involves maximal surgical resection and temozolomide (TMZ) chemotherapy, the discovery of novel anti-tumor agents from nature sources is an effective strategy for glioma treatment. In this study, we conducted a screening process to identify the bisindole alkaloid melodinine J (MDJ) from Melodinus tenuicaudatus.

Clinical Pharmacist Involved in the Treatment of Pneumonia: A Case Report.

Objective: The first-line treatment for severe pneumonia (PCP) is trimethoprim-sulfamethoxazole (TMP/SMZ). Here, we report a case involving 6-month-old child with a PCP infection, highlighting the role of clinical pharmacists in providing individualized pharmaceutical care and guidance through the process of therapeutic drug monitoring (TDM).

Methods: The clinical pharmacist monitored the concentration of TMP/SMZ in the serum, urine and sputum of a 6-month-old child with PCP infection.

The immune function of TLR4-1 gene in Octopus sinensis revealed by RNAi and RNA-seq.

Toll-like receptors (TLRs) are a class of conserved pattern recognition receptors (PRRs) that are crucial for initiating the innate immune response and aiding in the clearance of pathogenic organisms. Many studies have identified TLR4 as a distinctive member of the TLR family, capable of activating both the Myeloid differentiation factor 88-dependent signaling pathway (MyD88-dependent) and the TIR-domain-containing adaptor inducing IFN-β dependent signaling pathway (TRIF-dependent). Nevertheless, the role of TLR4 in Cephalopoda is still largely unexplored.

Photo-crosslinking methacrylated-amylopectin/polyacrylamide hydrogels loading curcumin for applications as degradable, injectable, and antibacterial wound dressings.

The preparation of biodegradable and antibacterial hydrogels has important clinical value. In this work, a novel strategy has been developed to prepare degradable hydrogel dressings without chemical crosslinking agent using methacrylate anhydride (MA)-modified amylopectin (APMA) and polyacrylamide (PAM). After introducing CC bonds, APMA/PAM hydrogels can be formed under light irradiation.