[Effects of catalpol, L-shikonin and paeonol extracted from radix rehmanniae, radix arnebiae and cortex moutan on KGF-induced HaCaT cell proliferation].

Objective: To explore the effects of Chinese herbal medicine radix rehmanniae, radix arnebiae and cortex moutan on the proliferation of HaCaT cells and explore their potential curative mechanisms.

Methods: The main monomers of catalpol, l-shikonin and paeonol were extracted. And 10 ng/ml keratinocyte growth factor (KGF) was used to induce HaCaT cell to build an in vitro model of hyperproliferation of epidermal keratinocytes.

Protection of skin with subcutaneous administration of 5% dextrose in water during superficial radiofrequency ablation in a rabbit model.

Purpose: This study was to evaluate the efficacy of subcutaneous administration of 5% dextrose in water (D5W), to prevent skin injury during radiofrequency (RF) ablation.

Materials And Methods: Twenty-four rabbits were divided into three groups: a pre-injection group, a perfusion group, and a control group. Ablative zones were created in the superficial part of the thigh muscle for 6 min.

Dissipative particle dynamics simulation study on vesicles self-assembled from amphiphilic hyperbranched multiarm copolymers.

Hyperbranched multiarm copolymers (HMCs) have been shown to hold great potential as precursors in self-assembly, and many impressive supramolecular structures have been prepared through the self-assembly of HMCs in solution. However, theoretical studies on the corresponding self-assembly mechanism have been greatly lagging behind. Herein, we report the self-assembly of normal or reverse vesicles from amphiphilic HMCs by dissipative particle dynamics (DPD) simulation.

Association between Neck/Shoulder Pain and Trapezius Muscle Tenderness in Office Workers.

Background. Neck/shoulder pain is a common musculoskeletal disorder among adults. The pain is often assumed to be related to muscular tenderness rather than serious chronic disease.

Effect of age and biological subtype on the risk and timing of brain metastasis in breast cancer patients.

Background: Brain metastasis is a major complication of breast cancer. This study aimed to analyze the effect of age and biological subtype on the risk and timing of brain metastasis in breast cancer patients.

Patients And Methods: We identified subtypes of invasive ductal carcinoma of the breast by determining estrogen receptor, progesterone receptor and HER2 status.

Effect of brief daily resistance training on occupational neck/shoulder muscle activity in office workers with chronic pain: randomized controlled trial.

Purpose: This study investigates the acute and longitudinal effects of resistance training on occupational muscle activity in office workers with chronic pain.

Methods: 30 female office workers with chronic neck and shoulder pain participated for 10 weeks in high-intensity elastic resistance training for 2 minutes per day (n = 15) or in control receiving weekly email-based information on general health (n = 15). Electromyography (EMG) from the splenius and upper trapezius was recorded during a normal workday.

Particle size concentration distribution and influences on exhaled breath particles in mechanically ventilated patients.

Humans produce exhaled breath particles (EBPs) during various breath activities, such as normal breathing, coughing, talking, and sneezing. Airborne transmission risk exists when EBPs have attached pathogens. Until recently, few investigations had evaluated the size and concentration distributions of EBPs from mechanically ventilated patients with different ventilation mode settings.

[The pathological observation of lung's draining lymph node on 12 autopsy with coal workers].

Objective: To investigate the pathological characteristics of draining pulmonary lymph nodes in coal workers' pneumoconiosis (CWP) and study their significance for the early diagnosis of CWP.

Methods: The autopsy materials of 12 coal workers were subjected to HE staining and observed under a light microscope, and a retrospective analysis was performed considering the occupational history.

Results: The age range of 12 cases was 46∼71 years (mean, 58 years), and the exposure time range was 3∼32 years (mean, 22.

[The influence of mitomycin C and paclitaxel on the proliferation and apoptosis of human pulmonary fibroblast].

Objective: To observe the inhibitory effect and potential mechanism of mitomycin C and paclitaxel on the proliferation of Human Pulmonary Fibroblast in vitro. So as to providing an experimental reference for the design of drug eluting airway stents.

Methods: Cell viability was measured by MTT assay after different concentrations of mitomycin C or paclitaxel varying from 10(-1)1 mol/L to 10(-4) mol/L had been applied to the fibroblasts for 24, 48 or 72 h, respectively.

Femtogram detection of cytokines in a direct dot-blot assay using SERS microspectroscopy and hydrophilically stabilized Au-Ag nanoshells.

Rapid parallel detection of two cytokines (IL-6 and IL-8) with femtogram sensitivity in a simple direct dot-blot assay is demonstrated. The microspectroscopic SERS acquisition scheme employs rationally designed, hydrophilically stabilized Au-Ag nanoshells as SERS labels, which are optimized for signal enhancement upon red laser excitation.

Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells.

Background: Therapeutic antibody development is one of the fastest growing areas of the pharmaceutical industry. Generating high-quality monoclonal antibodies against a given therapeutic target is crucial for successful drug development. However, due to immune tolerance, making it difficult to generate antibodies using conventional approaches.

A three-dimensional polymeric potassium complex of 5-sulfonobenzene-1,2,4-tricarboxylic acid: poly[μ-aqua-aqua-μ9-(2,4-dicarboxy-5-sulfonatobenzoato)-dipotassium(I)].

The asymmetric unit in the structure of the title compound, [K2(C9H4O9S)(H2O)2]n, consists of two eight-coordinated K(I) cations, one 2,4-dicarboxy-5-sulfonatobenzoate dianion (H2SBTC(2-)), one bridging water molecule and one terminal coordinated water molecule. One K(I) cation is coordinated by three carboxylate O atoms and three sulfonate O atoms from four H2SBTC(2-) ligands and by two bridging water molecules. The second K(I) cation is coordinated by four sulfonate O atoms and three carboxylate O atoms from five H2SBTC(2-) ligands and by one terminal coordinated water molecule.

Palladium-catalyzed C-2 selective arylation of quinolines.

An efficient method for the Pd-catalyzed regioselective C-2 arylation of quinolines is presented. Reactions of various substituted quinolines and unactivated arenes have been conducted under mild conditions. The result shows good product yields of 2-arylquinolines, which are highly useful building blocks for the synthesis of bioactive alkaloid natural products and drug molecules.

[The clinical pathological analysis of 14 autopsy cases of pneumoconiosis].

Objective: To investigate the histopathological features, complications, diagnosis, and differential diagnosis of coal workers' pneumoconiosis (CWP).

Methods: The lung tissue sections from 14 autopsy cases of CWP were subjected to HE staining and observed under a light microscope, and a retrospective analysis was performed considering the occupational history and clinical features.

Results: The 14 cases were 46-71 years of age (mean, 57.

[A method for the primary culture of fibroblasts isolated from human airway granulation tissues].

Objective: To establish a feasible method to culture primary fibroblasts isolated from human airway granulation tissues, and therefore to provide experimental data for the investigation of the pathogenesis of benign airway stenosis.

Methods: The granulation tissues were collected from 6 patients during routine bronchoscopy at our department of Beijing Tiantan Hospital from April to June 2011. Primary fibroblasts were obtained by culturing the explanted tissues.

[Numerical simulation and application of electrical resistivity survey in heavy metal contaminated sites].

In order to analyze the effects of electrical resistivity in heavy metal contaminated sites, we established the resistivity model of typical contaminated sites and simulate the DC resistivity method with Wenner arrays using the finite element method. The simulation results showed that the electrical method was influenced by the contamination concentration and the location of pollution. The more serious the degree of pollution was, the more obvious the low resistivity anomaly, thus the easier the identification of the contaminated area; otherwise, if there was light pollution, Wenner array could not get obvious low resistivity anomalies, so it would be hard to judge the contaminated area.

[3, 4- dinitro-furazan-based oxidation furazan acute and subchronic toxicity studies].

Objective: To study the 3, 4- dinitro-furazan-based oxidation furazan (DNTF) of sub-acute toxicity and chronic toxicity, to determine the acute toxicity classification DNTF, the nature of toxic effects and major target organ for the development provide the basis for occupational exposure limits.

Methods: ( 1) Acute toxicity: The oral gavage method once infected, symptoms of poisoning of animals observed to calculate the LD50DNTF and 95% confidence limits. ( 2) sub-chronic experiment: selection of 96 healthy SD rats were randomly divided into four groups, doses of 25, 56.

[Salicylic acid derivatives as simplified and novel GK small molecule activators].

Glucokinase (GK) is a new target for the treatment of type II diabetes mellitus (T2DM). In order to find a structure-simplified small molecule GK activator, 19 salicylic acid derivatives were designed and synthesized based on new lead compound (1). Experimental results showed that the potency of compound 8h is superior to control RO-28-0450 in GK activation.

Cholestane-3β, 5α, 6β-triol suppresses proliferation, migration, and invasion of human prostate cancer cells.

Oxysterols are oxidation products of cholesterol. Cholestane-3β, 5α, 6β-triol (abbreviated as triol) is one of the most abundant and active oxysterols. Here, we report that triol exhibits anti-cancer activity against human prostate cancer cells.

Chiral induction in the ionothermal synthesis of a 3D chiral heterometallic metal-organic framework constructed from achiral 1,4-naphthalenedicarboxylate.

A chiral heteometallic compound, [(EMIM)NaCu(1,4-ndc)2]n (1), constructed from the achiral 1,4-naphthalenedicarboxylate (1,4-ndc) ligand has been ionothermally synthesized and structurally and magnetically characterized. The chiral induction effect of the enantiopure 1-ethyl-3-methylimidazolium (EMIM) L-lactate additive in the ionothermal reaction is briefly discussed.

Rational design and synthesis of SERS labels.

SERS labels are a new class of nanotags for optical detection based on Raman scattering. Central advantages include their spectral multiplexing capacity due to the small line width of vibrational Raman bands, quantification based on spectral intensities, high photostability, minimization of autofluorescence from biological specimens via red to near-infrared (NIR) excitation, and the need for only a single laser excitation line. Current concepts for the rational design and synthesis of SERS labels are summarized in this review.

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells.

Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono- and tri-ethylene glycol (EG) spacers, respectively.

Surface-enhanced Raman spectroscopy at single-molecule scale and its implications in biology.

Single-molecule (SM) spectroscopy has been an exciting area of research offering significant promise and hope in the field of sensor development to detect targets at ultra-low levels down to SM resolution. To the experts and developers in the field of surface-enhanced Raman spectroscopy (SERS), this has often been a challenge and a significant opportunity for exploration. Needless to say, the opportunities and excitement of this multidisciplinary area impacts span the fields of physics, chemistry and engineering, along with a significant thrust in applications constituting areas in medicine, biology, environment and agriculture among others.

[Teratogenicity of 3, 4 two furazan-based oxidation furazan in rats].

Objective: To study the teratogenicity of new high-energy compounds, 3, 4 two furazan-based oxidation furazan (DNTF) and the impact on human health, occupational exposure limits were provided for the following research.

Methods: Pregnant SD rats were randomly divided into five groups by Standard teratogenicity test, including three dose groups (5.0, 15.