Modulating flavor and microbiota of Yunnan suancai through salt reduction with KCl and κ-Carrageenan.

Fermented vegetables like Yunnan suancai are traditionally pickled with high salt, raising health concerns regarding excessive sodium intake. This study evaluated the impact of reduced-salt pickling system, partially replacing NaCl incorporating KCl with κ-carrageenan (CG) mixture (30 %, 40 %, and 50 %), on the biochemical characteristics, microbial dynamics, and flavor profiles during suancai fermentation. Result revealed significant differences in flavor profiles across treatments, driven by variations in 7 non-volatile and 46 volatile compounds.

Wettability-Switchable Azobenzene Inverse Opal Film via Interfacial Engineering.

Precise control of surface wettability and droplet dynamics is essential for the development of intelligent interfaces and fluidic devices. However, most existing systems suffer from limited wettability modulation, an irreversible response, or complex fabrication. Herein, we report an azobenzene inverse opal film capable of reversible wettability and adhesion switching under alternating UV/vis irradiation.

Structure, evolution, and roles of MYB transcription factors proteins in secondary metabolite biosynthetic pathways and abiotic stresses responses in plants: a comprehensive review.

Unlike mobile organisms, plants are sessile and thus more vulnerable to environmental stressors. Among these, abiotic stress represents a major constraint that profoundly affects plant growth and development. To cope with these challenges, plants have evolved sophisticated adaptive mechanisms to enhance their stress resilience.

Eliminating Mobility-Thickness Dependence in Transparent Conductive Oxide Layer Growth: A Critical Nucleation Strategy.

Carrier mobility is a key parameter for transparent conductive oxide (TCO) layers. However, it shows significant thickness-dependent deterioration in the reports so far, making it challenging to obtain high-quality ultrathin TCO films. Here, a critical nucleation strategy (cns) is proposed, i.

Ground-state patterns in a Rydberg-dressed Bose-Einstein condensate with spin-orbit coupling and Lee-Huang-Yang correction.

Spontaneous symmetry breaking and self-organized pattern formation in nonlinear systems are fundamental phenomena with broad implications. Studying these effects in cold atomic gases, particularly through the optical control of laser-dressed Rydberg atoms, bridges atomic physics and modern optics. Here, we investigate the ground-state matter-wave pattern formation in cold Rydberg-dressed Bose-Einstein condensates (BECs) with considerations of the Lee-Huang-Yang (LHY) correction and Raman-induced spin-orbit coupling (SOC).

Variability and drivers of plankton community metabolism in Yazhou Bay: A spatial and seasonal assessment.

Planktonic carbon metabolism is often used as a critical indicator to assess the health of marine ecosystems and their carbon sequestration capacity. In this study, we examined the spatial variability and driving factors of plankton metabolism, including gross primary production (GPP), respiration (CR), and their balance, net community production (NCP), in Yazhou Bay, a representative tropical open bay. The results revealed that the distribution of GPP and CR in Yazhou Bay is influenced by terrestrial inputs, seasonal rainfall, and oceanic currents, resulting in pronounced seasonal and spatial variations.

A single nucleotide substitution in the SlMCT gene contributes to great morphological alternations in tomato.

Terpenoids, a group of metabolites, are important to plant development and color formation, and serve as valuable nutrients for humans. The enzyme 4-diphosphocytidyl- 2 C-methyl-D-erythritol cytidyltransferase (MCT) plays a pivotal role in the methylerythritol phosphate (MEP) pathway for terpenoid biosynthesis. However, the potential lethality of MCT mutants has hindered further exploration into its functional role in terpenoid metabolite families in plants.

Quantum phase transition and chaos of anomalous Dicke model in the thermodynamic limit.

We study the anomalous Dicke model from two different perspectives including the quantum theory and semiclassical approximation method. Due to the competition between both bidirectional couplings in this appealing model, it exhibits three distinct phases, i.e.

Polycystic ovary syndrome: The role of granulosa cell proliferation and apoptosis in disease development.

Polycystic ovary syndrome (PCOS) is the most prevalent ovarian endocrine disorder in infertile women and significantly impacts their health. Granulosa cell (GC) is the largest functional cell type in follicular development and plays a crucial role in follicle growth, differentiation, and maturation. Studies have shown that women with PCOS experience abnormal GC proliferation and apoptosis, which contribute to symptoms such as inflammation, irregular estrous cycles, hormonal imbalances, and follicular development arrest.

Imaging observation of allogeneic bone and bone-induced calcium phosphate biomaterials as supplements in the treatment of infectious bone defects.

ObjectiveTo compare osseous regeneration efficacy of allogeneic bone versus bone-induced calcium phosphate biomaterial (biological artificial material induced artificial bone, BAM-induced artificial bone) in treating tibial infected bone defects.MethodsThis study was a prospective study that enrolled participants hospitalized at Sichuan Orthopedic Hospital for tibial osteomyelitis. These subjects had undergone osteomyelitis surgery and presented with bone defects requiring bone grafting under controlled infection conditions.

Analysis of Multiplexed Flow Cytometric Assays and Toxicogenomic Signatures for Genotoxicity Prediction: A Model Performance and Case Study Approach.

Genotoxicity assays play a significant role in protecting clinical trial subjects from potential increased risk of genotoxic hazard and cancer during drug development. Traditional genetic toxicology assays typically provide binary outcomes with limited mechanistic insight. This study evaluates machine learning (ML) models based on an in-house implementation of MultiFlow DNA Damage Assay and MicroFlow Assays, and compared these results to previously published Litron assays.

Development of Polysaccharide-Stabilized Selenium Disulfide Nanoparticles for Enhanced Antioxidant and Antimicrobial Applications in Dermatology.

Selenium disulfide (SeS) is a promising therapeutic agent for dermatological conditions, including dandruff and pruritus, but its clinical application is limited by poor solubility and stability. To address these challenges, we developed selenium disulfide nanoparticles stabilized by polysaccharides from (SPS-SeSNPs) for enhanced aqueous dispersion and evaluated their antioxidant properties, antibacterial efficacy, and dermal safety. The synthesized SPS-SeSNPs exhibited an average hydrodynamic diameter of 115.

STIM1 functionally couples to transient receptor potential ankyrin 1 contributing to nociception.

STIM1 is a calcium sensor that can sense calcium level changes in the endoplasmic reticulum (ER) and respond to extracellular stimuli. We have reported that STIM1 is expressed in nociceptors. However, its functional significance remains unclear.

Interfacial Coordination Engineering to Boost Li-Ion Conduction in Economic Zr-Based Halide Electrolytes.

Halide solid electrolytes (HSEs) have seen rapid progress in the development of all-solid-state lithium batteries (ASSLBs), offering favorable lithium-ion transport properties, broad electrochemical stability, and strong interfacial compatibility with high-voltage oxide cathodes. However, developing HSEs that simultaneously offer high ionic conductivity and low cost remains a significant challenge. Most high-conductivity halides rely on expensive metal elements, whereas cost-effective Zr-based halides are limited by their relatively low ionic conductivity.

Directly measured high in-plane thermal conductivity of two-dimensional covalent organic frameworks.

Two-dimensional covalent organic frameworks are promising low-density porous materials for lightweight thermal management, yet comprehensive thermal conductivity measurements remain scarce. Particularly, direct in-plane thermal conductivity data for large-area, fully suspended covalent organic framework thin films has not been reported previously. This study addresses this gap by measuring in-plane and cross-plane thermal conductivities of two-dimensional covalent organic frameworks with varying pore sizes using laser-based pump-probe techniques.

Integration of COSMO-RS and ANN-GA for extraction of echinacoside and acteoside from Cistanche deserticola using natural deep eutectic solvents.

A novel method for green extraction of echinacoside and acteoside from Cistanche deserticola (C. deserticola) using natural deep eutectic solvents (NADES) has been developed, integrating conductor-like screening model for realistic solvents (COSMO-RS) and artificial neural network-genetic algorithm (ANN-GA) optimization to maximize efficiency. Among 48 NADES evaluated, choline chloride-propylene glycol (1:3 M ratio, 30 wt% water content) was identified as the optimal solvent through COSMO-RS and single-factor optimization.

Electrochemical Engineering of Emerging 2D Materials beyond Graphene: Progress and Prospects.

Following the remarkable success of graphene, graphene-like 2D materials have garnered significant attention over the past decade due to their extraordinary physical and chemical properties. Despite the high demand for these materials in industry, developing facile, and cost-effective methods for large-scale production and functionalization under ambient conditions remains a challenge. As innovative green growth and modification strategies, electrochemical engineering techniques provide economic advantages in scalable manufacturing and surface manipulation.

Safety and efficacy of ciprofol for general anesthesia induction in elderly patients undergoing lumbar surgery.

Objective: This study aimed to evaluate the safety and efficacy of ciprofol for anesthesia induction in elderly patients undergoing lumbar surgery.

Methods: Sixty patients aged 65-80 years scheduled for elective lumbar surgery under general anesthesia were randomly divided into two groups ( = 30 each): ciprofol group and propofol group. The ciprofol group received intravenous ciprofol 0.

Investigating Hepatic Glucose Metabolism in Insulin Resistance Cell Model Using 3D-Printed Open Microfluidic Chip.

The liver is recognized as a pivotal tissue contributing to insulin resistance and exerts a crucial influence on the systemic orchestration of glucose metabolism. While biologists have embraced microfluidics for its potential to elucidate hepatic glucose homeostasis and metabolic dynamics, huge challenges remain in the widespread adoption of microfluidic devices, such as complex design and production, and the use of additional pumps for fluid handling. In this study, we report the fabrication of an α-cellulose-modified open microfluidic chip using a 3D printer for the investigation of glucose metabolism in HepG2 cells after insulin and pioglitazone intervention.

Integration of Pharmacodynamics, Proteomics, and Metabolomics to Elucidate Effect and Mechanism of Rehmanniae Radix in the Treatment of T2DM.

Rehmanniae Radix (RR) has a long history in treating type 2 diabetes mellitus (T2DM). It was previously shown to alleviate T2DM through the regulation of molecular targets in the kidney. However, the role of RR on T2DM-driven hepatic metabolism disorder remains unclear.

Resveratrol mitigated acute sleep deprivation-induced cognitive impairment by suppressing hippocampal neuronal ferroptosis.

Sleep deprivation (SD) induces extensive neuropsychiatric disorders, including cognitive impairment, which reduce the life quality of the victim and increase the burden on the healthcare system. The potential underlying mechanism and promising preventions for sleep deprivation-induced cognitive impairment remain to be further explored. Our preliminary study found that sleep deprivation activated ferroptosis in mice hippocampi.

Association Between Number of Temporomandibular Disorders Symptoms and Oral Health Quality of Life: A Cross-Sectional Study in a Community-Based Population.

Background: There are limited studies on the number of Temporomandibular disorders (TMD) symptoms affecting oral health-related quality of life (OHRQoL) in large community populations. Furthermore, there is a lack of comparative studies between different TMD subtypes.

Objective: The aim was to explore the relationship between OHRQoL and the number of TMD symptoms in a community-based population.

Single Antisense Oligonucleotides Correct Diverse Splicing Mutations in Hotspot Exons.

Mutations that impact splicing play a significant role in disease etiology but are not fully understood. To characterize the impact of exonic variants on splicing in 71 clinically actionable disease genes in asymptomatic people, we analyzed 32,112 exonic mutations from ClinVar and Geisinger MyCode using a minigene reporter assay. We identify 1,733 splice-disrupting mutations, with the most extreme variants likely being deleterious.