Niche Adaptation of Ammonia-Oxidizing Bacteria in Partial Nitrification System: Keystone Species and Unique Survival Strategies.

Partial nitrification (PN) is of practical significance for achieving autotrophic nitrogen removal with impressive economic and environmental benefits. While most studies focused on the selective suppression of nitrite-oxidizing bacteria, maintaining stable ammonia oxidation as the primary source of nitrite remains equally critical but largely underexplored. Herein, we identified as the keystone species dominating ammonia oxidation in free ammonia treatment-based PN systems, comprising over 40% of the nitrifier community.

Anaerobic digestion under perfluorooctane sulfonate stress: Mechanistic insights into methane production inhibition, microbial responses, and functional adaptations.

Perfluorooctane sulfonate (PFOS) is a highly persistent and cytotoxic emerging pollutant that accumulates in sludge. Clarifying its effects on anaerobic digestion and its cytotoxicity on functional microbial metabolism is essential for evaluating the ecological risks it may pose during sludge disposal. This study investigated the effects of PFOS on methane production, microbial community responses and functional adaptations during anaerobic digestion.

Unraveling the dual role in enhancing methane production and mitigating antibiotic resistance gene spread in anaerobic co-digestion of microalgae and waste activated sludge.

Waste activated sludge (WAS) is a double-edged sword - a recognized repository for antibiotic resistance genes (ARGs) but also a renewable substrate for methane production. Developing effective WAS treatment strategies is therefore of both ecological and practical importance. In this study, we proposed an anaerobic co-digestion approach of WAS and microalgae Chlorella sp.

Biogas production enhancement from anaerobic digestion with magnetic biochar: Insights into the functional microbes and DIET.

The application of magnetic biochar in anaerobic digestion (AD) has gained increasing attention. However, the underlying mechanisms remain insufficiently understood. This study systematically investigated the effects of magnetic biochar on functional microbial communities involved in methanogenesis and elucidated its role in promoting direct interspecies electron transfer (DIET) within AD systems.

Aspartame affects methane yield and enhances transmission of antibiotic resistance genes during anaerobic digestion of sludge.

Aspartame (ASP) is a widely used artificial sweetener, yet recent studies have shown that ASP have potential toxic effect. ASP is also detected in sludge, however, the influence of ASP on the performance of sludge anaerobic digestion and the fate of antibiotic resistance genes (ARGs) have not been thoroughly investigated. Under stress of 0, 0.

Microplastics enhance the adsorption capacity of zinc oxide nanoparticles: Interactive mechanisms and influence factors.

Microplastics (MPs) are of particular concern due to their ubiquitous occurrence and propensity to interact and concentrate various waterborne contaminants from aqueous surroundings. Studies on the interaction and joint toxicity of MPs on engineered nanoparticles (ENPs) are exhaustive, but limited research on the effect of MPs on the properties of ENPs in multi-solute systems. Here, the effect of MPs on adsorption ability of ENPs to antibiotics was investigated for the first time.

Co-occurrence of dominant bacteria and methanogenic archaea and their metabolic traits in a thermophilic anaerobic digester.

Thermophilic anaerobic digestion (TAD) represents a promising biotechnology for both methane energy production and waste stream treatment. However, numerous critical microorganisms and their metabolic characteristics involved in this process remain unidentified due to the limitations of culturable isolates. This study investigated the phylogenetic composition and potential metabolic traits of bacteria and methanogenic archaea in a TAD system using culture-independent metagenomics.

Bisphenol A affects microbial interactions and metabolic responses in sludge anaerobic digestion.

The widespread use of bisphenol A (BPA) has resulted in the emergence of new pollutants in various environments, particularly concentrated in sewage sludge. This study investigated the effects of BPA on sludge anaerobic digestion, focusing specifically on the interaction of microbial communities and their metabolic responses. While the influence of BPA on methane accumulation is not significant, BPA still enhanced the conversion of soluble COD, protein, and polysaccharides.

Metagenomic insights into the toxicity of carbamazepine to functional microorganisms in sludge anaerobic digestion.

Contaminants of emerging concern (CECs) contained in sludge, such as carbamazepine, may be toxic to microorganisms and affect the biogenesis of methane during anaerobic digestion. In this study, different scales of anaerobic digesters were constructed to investigate the inhibitory effect of carbamazepine. Results showed that carbamazepine reduced methane production by 11.

Carbamazepine facilitated horizontal transfer of antibiotic resistance genes by enhancing microbial communication and aggregation.

Antimicrobial resistance is a global health security issue of widespread concern. Recent studies have unveiled the potential contribution of non-antibiotics to the emergence of antimicrobial resistance. This study investigated the effect of carbamazepine, a non-antibiotic pharmaceutical, on the fate of antibiotic resistance genes (ARGs) during anaerobic digestion.

Deregulation of Ribosome Biogenesis in Nitrite-Oxidizing Bacteria Leads to Nitrite Accumulation.

Nitrite (NO) accumulation caused by nitrite-oxidizing bacteria (NOB) inhibition in nitrification is a double-edged sword, i.e., a disaster in aquatic environments but a hope for innovating nitrogen removal technology in wastewater treatment.

Magnetic biochar promotes the risk of mobile genetic elements propagation in sludge anaerobic digestion.

Mobile genetic elements (MGEs) mediated horizontal gene transfer is the primary reason for the propagation of antibiotic resistance genes in environment. The behavior of MGEs under magnetic biochar pressure in sludge anaerobic digestion (AD) is still unknown. This study evaluated the effects of different dosage magnetic biochar on the MGEs in AD reactors.

Microplastics influence the fate of antibiotics in freshwater environments: Biofilm formation and its effect on adsorption behavior.

Microplastics (MPs) are substrates available for biofilms colonization in natural water environments. The biofilms formation may enhance the ability of MPs to adsorb harmful contaminants. Herein, we investigated the biofilms formation of three different MPs (PVC, PA and HDPE) in simulated natural environment, and observed the chemical structure, charge property, hydrophobicity and other properties of MPs affect microbial biomass and community composition.

Advanced MOFs@aerogel composites: Construction and application towards environmental remediation.

Environmental pollution has drawn forth advanced materials and progressive techniques concentrating on sustainable development. Metal-organic frameworks (MOFs) have aroused vast interest resulting from their excellent property in structure and function. Conversely, powdery MOFs in highly crystalline follow with fragility, poor processability and recoverability.

Biofilm on microplastics in aqueous environment: Physicochemical properties and environmental implications.

The excellent properties of plastics make them widely used all over the world. However, when plastics enter the environmental medium, microplastics will inevitably be produced due to physical, chemical and biological factors. Studies have shown that microplastics have been detected in terrestrial, aquatic and atmospheric environments.

Facile synthesis of recyclable 3D gelatin aerogel decorated with MIL-88B(Fe) for activation peroxydisulfate degradation of norfloxacin.

The application of traditional powder catalysts is limited by particle agglomeration and difficult recovery. In this work, a three-dimensional porous aerogel catalyst for organic pollutants degradation in water by activating peroxydisulfate (PDS) was successfully synthesized, which was obtained via directly mixing of MIL-88B(Fe) with sol precursors followed by vacuum freeze-drying and low-temperature calcination. MIL-88B(Fe)/gelatin aerogel-150/PDS (MGA-150/PDS) system displayed satisfactory norfloxacin (NOR) degradation performance, which could remove 98.

Addition of nanoparticles increases the abundance of mobile genetic elements and changes microbial community in the sludge anaerobic digestion system.

This study explored the fate of mobile genetic elements (MGEs) in anaerobic digestion (AD) system with four nanoparticles (NPs) added, including carbon NPs, AlO NPs, ZnO NPs, and CuO NPs. 16S rRNA amplicon sequencing and quantitative PCR to investigate the microbial community, MGEs abundance and the potential host in the AD process. The results of high-throughput sequencing showed that ZnO NPs and CuO NPs significantly reduced the microbial diversity and significantly changed the microbial community structure.

Co-composting of sewage sludge and Phragmites australis using different insulating strategies.

Insulating strategies are indispensable for laboratory-scale composting reactors, however, current insulation methods interfere with the aerobic fermentation behaviors related to composting. To address this issue, a centre-oriented real-time temperature compensation strategy was designed in this study. Five 9 L reactors (R1-R5) with different insulation strategies were used for the co-composting of dewatered sludge and Phragmites australis and compared.

Evolutions of antibiotic resistance genes (ARGs), class 1 integron-integrase (intI1) and potential hosts of ARGs during sludge anaerobic digestion with the iron nanoparticles addition.

In this work, we investigated the impact of iron nanoparticle, including magnetite nanoparticles (FeO NPs) and nanoscale zero-valent iron (nZVI), on the anaerobic digestion (AD) performance. Moreover, the evolutions of antibiotic resistance genes (ARGs), class 1 integrons-integrase (intI1) and potential hosts of ARGs were also investigated. The optimal addition of FeO NPs and nZVI to promote methane production was 0.

Effects of different conductive nanomaterials on anaerobic digestion process and microbial community of sludge.

The effects of four conductive nanomaterials (nano-carbon powder, nano-AlO, nano-ZnO, nano-CuO) on sludge anaerobic digestion (AD) performance and microbial community were investigated through a 36-day fermentation experiment. Results showed that biogas production enhanced by 16.9% and 23.

Influence of nanoscale zero-valent iron and magnetite nanoparticles on anaerobic digestion performance and macrolide, aminoglycoside, β-lactam resistance genes reduction.

The effect of nanoscale zero-valent iron (NZVI) and magnetite nanoparticles (FeO NPs) on anaerobic digestion (AD) performance was investigated through a series of 100-day semi-continuous mesophilic anaerobic digestions. The results indicated that biogas production had increased by 24.44% and 21.

Enhanced mesophilic anaerobic digestion of waste sludge with the iron nanoparticles addition and kinetic analysis.

As the functional material, iron nanoparticles effectively promote anaerobic digestion (AD) process, including the hydrolysis-acidification process and the biogas production. In this study, nano zero-valent iron (nZVI) and FeO nanoparticles (FeO NPs) were added to AD reactors respectively. The AD process was evaluated by the reactors performances, including pH, biogas yields and compositions, as well as the removal ratio of total solids (TS), volatile solids (VS) and soluble chemical oxygen demand (sCOD).

Halide Ion-Induced Switching of Gold Nanozyme Activity Based on Au-X Interactions.

The influence of halide ion on the peroxidase activity of protein-modified gold nanoparticles (AuNPs) has been explored, based on the Au-X interaction directed binding of halide ion to AuNPs. Due to different Au-X interactions, halide has been demonstrated to display different switching behaviors to the catalytic activity of protein-modified AuNPs. Presented is the finding that iodide can rapidly inhibit the enzyme activity of CM-AuNP nanozyme effectively.

Adsorption Behavior of Low-Concentration Imidazolium-Based Ionic Liquid Surfactant on Silica Nanoparticles.

The adsorption behavior of imidazolium-based ionic liquid surfactant ([C12mim]Br) on silica nanoparticles (NPs) has been studied with turbidity, isothermal titration microcalorimetry, fluorescence spectroscopy, and dynamic light scattering (DLS) measurements. Both the electrostatic attraction and the hydrogen bonding interaction between silica NP and [C12mim]Br play crucial roles during [C12mim]Br monomers binding to silica NPs at low surfactant concentration, and the hydrophobic effect leads to formation of micelle-like aggregates on silica NP surfaces with the further increase of surfactant concentration. Furthermore, it is found that sodium halide salts favor the adsorption of [C12mim]Br on silica NP surfaces by decreasing the electrostatic repulsions.

Synthesis, structure-activity relationship analysis and kinetics study of reductive derivatives of flavonoids as Helicobacter pylori urease inhibitors.

In a continuing study for discovering urease inhibitors based on flavonoids, nineteen reductive derivatives of flavonoids were synthesized and evaluated against Helicobacter pylori urease. Analysis of structure-activity relationship disclosed that 4-deoxy analogues are more potent than other reductive products. Out of them, 4',7,8-trihydroxyl-2-isoflavene (13) was found to be the most active with IC50 of 0.