Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China.

The present study reports a rare case of Taenia saginata infection, which was initially diagnosed as acute cholecystitis in a Tibetan patient at the Qinghai-Tibetan Plateau pastoral area, China. A 45-year-old female was initially diagnosed with acute cholecystitis at a hospital in China. She had a slight fever, weight loss and constipation and complained of pain in the upper abdomen and left back areas.

Coenurosis of Yak, Bos grunniens, caused by Taenia multiceps: A Case Report with Molecular Identification in Qinghai Tibetan Plateau Area, China.

Coenurosis is an important zoonotic helminthic disease caused by the larval stage of the tapeworm Taenia multiceps. This parasite typically infects the brain of the intermediate hosts, including sheep, goat, cattle and even humans. We report a case of T.

The First Case of Diarrhoea in Tibetan Sheep, Ovis aries, Caused by Balantidium coli in the Qinghai Tibetan Plateau Area, China.

This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles.

[Genotyping and gene nucleotide polymorphism of epidemic strain of metacestode from humans and sheep in Tianjun region, Qinghai Province].

Objective: To understand the genotypes and nucleotide polymorphisms of metacestode from humans and sheep in Tianjun region, Qinghai Province.

Methods: The specific primers were designed according to the cox1 and nad1 genes of mitochondrial genome sequences accessed by GenBank. The primers were used to detect the cyst samples from 16 sheep and 2 humans infected with in Tianjun region of Qinghai Province by PCR, then the PCR amplification products were sequenced, the genotypes and nucleotide polymorphisms of the cox1 and nad1 genes were analyzed.

A Case of Coenurosis in a Wild Rabbit () Caused by Metacestode in Qinghai Tibetan Plateau Area, China.

Six cystic metacestodes were found in the abdominal muscles of a wild rabbit, , in China. The coenurus contained one or more scolices armed with hooklets. Mitochondrial 1 (1,623 bp) confirmed 98% homology with 1 of .

Lasiopodomys fuscus as an important intermediate host for Echinococcus multilocularis: isolation and phylogenetic identification of the parasite.

Background: Echinococcus multilocularis causes alveolar echinococcosis (AE) and is widely prevalent in Qinghai Province, China, where a number of different species have been identified as hosts. However, limited information is available on the Qinghai vole (Lasiopodomys fuscus), which is hyper endemic to Qinghai Province and may represent a potential intermediate host of E. multilocularis.

[Epidemic status of alveolar echinococcosis in Tibetan children in south Qinghai Province].

Objective: To understand the epidemic status of alveolar echinococcosis in Tibetan children in south Qinghai Province.

Methods: The imageology and serology methods were applied to investigate the epidemic status of alveolar echinococcosis of elementary school students in Yushu and Guoluo prefectures.

Results: Totally 19 629 children were investigated by the portable B ultrasound and 221 children were diagnosed as alveolar echinococcosis with the morbidity of 1.

Analyses on the misdiagnoses of 25 patients with unilateral optic nerve sheath meningioma.

Aim: To investigate clinical features of optic nerve sheath meningioma (ONSM) that was misdiagnosed, and to find methods to reduce the misdiagnoses.

Methods: Retrospective series study. Twenty-five misdisgnosed patients with unilateral ONSM were collected from Jan.

Promotion on the differentiation of retinal Müller cells into retinal ganglion cells by Brn-3b.

Aim: To investigate the role of Brn-3b in differentiation process of stem cells derived from retinal Müller cells into the ganglion cell.

Methods: The passage culture method of Müller cells from retina of newborn Sprague Dawley rats was carried out by repeated incomplete pancreatic enzyme digestion method. The cells were detected by fluorescence-activated cell sorter (FACS), immunohistochemistry technology and reverse transcription-polymerase chain reaction (RT-PCR) to determine the purity.

Atoh7 promotes the differentiation of Müller cells-derived retinal stem cells into retinal ganglion cells in a rat model of glaucoma.

Glaucoma is one of the leading eye diseases resulting in blindness due to the death of retinal ganglion cells. This study aimed to develop novel protocol to promote the differentiation of retinal Müller cells into ganglion cells in vivo in a rat model of glaucoma. The stem cells dedifferentiated from rat retinal Müller cells were randomized to receive transfection with empty lentivirus PGC-FU-GFP or lentivirus PGC-FU-Atoh7-GFP, or no transfection.

TaUBA, a UBA domain-containing protein in wheat (Triticum aestivum L.), is a negative regulator of salt and drought stress response in transgenic Arabidopsis.

TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blot suggested that TaUBA is a low copy gene in common wheat.

Comparison of two methods used to culture and purify rat retinal Müller cells.

Aim: To study two methods for culturing and purifying Sprague-Dawley (SD) rat retinal Müller cells and determine which one is better.

Methods: The passage culture method of Müller cells was respectively carried out by complete pancreatic enzyme digestion method and repeated incomplete pancreatic enzyme digestion method. After culturing retinal cells for one month through these two methods, fluorescence-activated cell sorter (FACS), RT-PCR, and immunohistochemistry technology were performed to examine the enrichment and purity of Müller glial cells, and carried out two-sample approximate t test using SSPS 13.

Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signaling.

Introduction: Retinal Müller cells exhibit the characteristics of retinal progenitor cells, and differentiate into ganglion cells under certain conditions. However, the number of ganglion cells differentiated from retinal Müller cells falls far short of therapeutic needs. This study aimed to develop a novel protocol to promote the differentiation of retinal Müller cells into ganglion cells and explore the underlying signaling mechanisms.

Tunable photonic bandgap in a one-dimensional superconducting-dielectric superlattice.

The transmittance of one-dimensional photonic crystals consisting of superconductor and lossless dielectric has been systematically studied through the transfer-matrix method. Obviously, the shift of the photonic bandgap (PBG) becomes more noticeable by adjusting the thicknesses of the dielectric layers than that of superconductor layers. Furthermore, the number of PBGs can be controlled by varying the thicknesses of dielectric layers.

[Screening of the candidate proteins interacting with TaFRA].

F-box protein is an important subunit of SCF complex, an E3 ligase in ubiquitin system, and its function is determined through mediating the specific recognition and combining with substrate protein. TaFRA (F-box protein related to abiotic stress) was identified by RACE based on the fragments diferently expressing in wheat seedling exposed to salt stress and encodes an F-box protein. In this study, pBD-TaFRA bait expression vector was constructed, and cDNA+pGAD+pBD was directly co-transformed into yeast hybrid system to screen condidate proteins interacting with TaFRA.

Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen-use- and yield-related traits in bread wheat.

Glutamine synthetase (GS) plays a key role in the growth, nitrogen (N) use and yield potential of cereal crops. Investigating the haplotype variation of GS genes and its association with agronomic traits may provide useful information for improving wheat N-use efficiency and yield. We isolated the promoter and coding region sequences of the plastic glutamine synthetase isoform (GS2) genes located on chromosomes 2A, 2B and 2D in bread wheat.

[FISH analysis of a CRW-homology sequence from Pseudoroegneria spicata in Thinopyrum ponticum and Th. intermedium].

To verify the genome components of Thinopyrum ponticum Liu alt; Wang and Th. intermedium [Host] Barkworth alt; Dewey, six specific primer pairs were designed according to the sequence of an autonomous centromeric retrotransposon of wheat (CRW) from Triticum boeoticum. Several DNA fragments were amplified by PCR from the genomic DNA of the diploid species Pseudoroegneria spicata A Löve.

[Application of ICP-AES to detecting nutrients in grain of wheat core collection of China].

Deficiency of micronutrients, especially iron and zinc, has been a serious malnutrition problem worldwide in human health. Increasing Fe and Zn concentrations in grains by means of plant breeding is a sustainable, effective and important way to improve human mineral nutrition and health. However, little information on grain Fe and Zn concentrations in Chinese wheat genotypes is available.

Isolation and molecular characterization of high molecular weight glutenin subunit genes 1Bx13 and 1By16 from hexaploid wheat.

The high molecular weight glutenin subunit (HMW-GS) pair 1Bx13 + 1By16are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism by which 1By16 and 1Bx13 creates high quality, their open reading frames (ORFs) were amplified from common wheat Atlas66 and Jimai 20 using primers that were designed based on published sequences of HMW glutenin genes. The ORF of 1By16 was 2,220 bp, deduced into 738 amino acid residues with seven cysteines including 59 hexapeptides and 22 nanopeptides motifs.

[Identification of important genes by marker-trait association analysis based on hitchhiking mapping].

Most agronomic traits such as yield, quality and stress-tolerance of crops are quantitative traits. It is not easy to dissect genetic basis of these traits because they are controlled by multi-genes that are affected by environmental factors. Hitchhiking mapping offers a new method for identifying of loci controlling those traits and assessing their allelic variations.

[The function of F-box protein in plant growth and development].

Ubiquitin-mediated proteolysis is involved in many biological processes in eukaryotes. SCF complex is a very important ubiquitin E3 ligase which has been exploited very well in plants. F-box protein characterized by an F-box motif is a subunit of SCF complex, which works as determinant in substrate recognition.

[Expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis].

Objective: To study the expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis.

Methods: Three tumor cell lines were used in this study: gastric cancer cell lines AGS, SGC7901 and hepatocellular carcinoma cell line HepG2. Expression level of Rac1 mRNA was detected by semi-quantitative RT-PCR.

[Screening of bioactive peptide that mimic the epitope of gastric cancer associated antigen].

Aim: To screen bioactive peptides that mimic the epitope of gastric cancer associated antigen.

Methods: Anti-gastric cancer monoclonal antibody (mAb) MG7 was purified by ion chromatography, and then coated on ELISA plate. By using the mAb as selective molecular, a 12-meres phage displaying peptide library was biopanned, and the positive clones were selected.

Coupled anaerobic/aerobic biodegradation of 2,4,6 trichlorophenol.

Degradation of 2,4,6-trichlorophenol (TCP) with co-immobilizing anaerobic granular sludge and isolated aerobic bacterial species was studied in coupled anaerobic/aerobic integrated reactors. The synergism of aerobes and anaerobes within co-immobilized granule might facilitate degrading the TCP and exchange of anaerobic metabolites 4-CP, which promoted system organic removal efficiency and recovered from organic shock-loads more quickly. The biomass specific activities experiment further confirmed that strict anaerobes be not affected over the course of this experiment by the presence of an oxic environment, aerobic activity predominated in the outer co-immobilized granule layers, while the interior was characterized by anaerobic activity.

Expression and bioactivity identification of soluble MG7 scFv.

Aim: To examine the molecular mass and identify the bioactivity of MG7 scFv for its application as a targeting mediator in gene therapy of gastric cancer.

Methods: Two strongly positive recombinant phage clones screened from MG7 recombinant phage antibody library were separately transfected into E.coli TG1.