Coordination-driven immobilization of his-tagged superoxide dismutase fusion protein on UIO-66-NH for enhanced photocatalytic hydrogen peroxide production.

Efficiently enhancing hydrogen peroxide (HO) production via photocatalysis remains challenging. This study employed genetic engineering to express His-tagged superoxide dismutase (His-SOD), which was selectively anchored onto the coordinatively unsaturated metal sites of etched UIO-66-NH₂ (UIO-66-NH) through coordination-driven assembly. The synergistic effect between His-SOD and UIO-66-NH significantly enhanced both the stability and substrate affinity of the enzyme, while simultaneously boosting the photocatalytic activity of the MOF.

An integrated purification and immobilization strategy for ELP-fusion β-glucosidase by its thermosensitivity and hydrophobicity.

In the field of biocatalysis, enzymes play a crucial role. However, they are faced with many challenges in practical applications, such as poor operational stability, high production costs and difficulties in recycling. Therefore, the efficient separation, purification and immobilization of enzymes are key to realize their industrial application.

Surgical Advances in the Treatment of Acromioclavicular Joint Injury: A Comprehensive Review.

In this article, we aim to review the diagnosis, classification, and treatment of acromioclavicular joint injury by mainly describing the progress of surgical treatment for acromioclavicular joint injury, providing a reference for clinical diagnosis and treatment. Acromioclavicular joint injury is a common sports injury, which is more common in male than in female patients. The mechanism of the injury is mainly caused by direct or indirect violence, which can be clearly diagnosed by radiography.

"Microflower-Templated" Janus Sheets: Synthesis and Application in Stabilizing Foams.

In this study, we proposed a method for fabricating Janus sheets using biological "microflowers" as a sacrificial template. The microflower-templated Janus sheets (MF-JNSs) were employed as a foam stabilizer in foam separation of the whey soybean protein (WSP). The MF-JNSs took inorganic hybrid microflowers (BSA@Cu (PO)-MF) as template, followed by the sequential attachment of protamine and silica to the surface of the BSA@Cu(PO)-MF.

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Lipase Immobilization.

In this work, a trimetallic (Ni/Co/Zn) organic framework (tMOF), synthesized by a solvothermal method, was calcinated at 400 and 600 °C and the final products were used as a support for lipase immobilization. The material annealed at 400 °C (Ni-Co-Zn@400) had an improved surface area (66.01 m/g) and pore volume (0.

Temperature-Sensitive Janus Particles PEG/SiO/PNIPAM-PEA: Applications in Foam Stabilization and Defoaming.

The current study presents a scalable approach for the preparation of temperature-responsive PEG/SiO/PNIPAM-PEA Janus particles and, for the first time, investigates their potential applications in stabilizing foam and defoaming by adjusting the temperature. The method utilizes a (W + O)/W emulsion system, which incorporates appropriate surfactants to stabilize the emulsion and prevent rapid dissolution of the hydrophilic triblock polymer PEG--PTEPM--PNIPAM in water. The PEG/SiO/PNIPAM-PEA Janus particles with temperature-responsive characteristics were synthesized in a single step that combined the sol-gel reaction and photoinduced free radical polymerization.

Amorphous-crystalline phase transition and intrinsic magnetic property of nickel organic framework for easy immobilization and recycling of β-Galactosidase.

This work describes the synthesis of fibrous nickel-based metal organic framework (Ni-ZIF) via simple solvothermal method. The material formed was calcinated at 400, 600, 800 °C to improve its surface area, porosity and enzyme binding capacity. Changes in X-ray diffraction pattern after calcination revealed the Ni-ZIF transitioned from amorphous to crystalline structure.

A novel immobilized horseradish peroxidase platform driven by visible light for the complete mineralization of sulfadiazine in water.

A novel immobilized enzyme driven by visible light was prepared and used for complete mineralization of antibiotics in water bodies. The immobilized enzyme was composed of carbon nitride modified by biochar (C/CN) and horseradish peroxidase (HRP), establishing the photo-enzyme coupling system with synergistic effect. Among them, the introduction of biochar not only improves the stability and loading capacity of the enzyme, but also improves the light absorption capacity and carrier separation efficiency of the photocatalyst.

Implant loosening following THA with S-ROM prosthesis and subtrochanteric osteotomy: Three case reports.

Prosthesis loosening after THA is a rather common complication. For DDH patients with Crowe IV, the surgical risk and complexity is significant. THA with S-ROM prosthesis combined with subtrochanteric osteotomy is a common treatment.

Therapeutic effects of saponins in rheumatoid arthritis: network pharmacology and experimental validation.

saponins (PNS) have been reported to have good anti-inflammatory effects. However, the anti-inflammatory effect mechanism in rheumatoid arthritis (RA) remains unknown. The focus of this research was to investigate the molecular mechanism of PNS in the treatment of RA.

Novel Recyclable UCST-Type Immobilized Glucose Isomerase Biocatalyst with Excellent Performance for Isomerization of Glucose to Fructose.

The development of a suitable immobilization strategy to improve the performance of immobilized glucose isomerase for the isomerization of glucose to fructose is crucial to promoting the industrial production of high-fructose syrup. In this work, a novel recyclable upper critical solution temperature (UCST)-type mVBA--P(AAm--AN)@glucose isomerase biocatalyst (PVAA@GI) was prepared, and the immobilized glucose isomerase could capture the glucose substrate through the affinity of 4-vinylbenzeneboronic acid (4-VBA) and the glucose substrate, which led to the enhanced substrate affinity and catalytic efficiency of the PVAA@GI. The biocatalyst exhibited excellent stability in pH, thermal, storage, and recycling compared to the free enzyme.

Silicon-substituted calcium phosphate promotes osteogenic-angiogenic coupling by activating the TLR4/PI3K/AKT signaling axis.

Silicon-substituted calcium phosphate (Si-CaP) is a promising bioactive material for bone tissue engineering. The mechanism of Si-CaP regulates osteogenic-angiogenic coupling during bone regeneration has not been fully elucidated. In this study, we screened the targets of Si-CaP and osteogenic-angiogenic coupling.

The potential mechanism of Fructus Ligustri Lucidi promoting osteogenetic differentiation of bone marrow mesenchymal stem cells based on network pharmacology, molecular docking and experimental identification.

Recent studies have shown that the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteogenic lineages can promotes bone formation and maintains bone homeostasis, which has become a promising therapeutic strategy for skeletal diseases such as osteoporosis. Fructus Ligustri Lucidi (FLL) has been widely used for the treatment of osteoporosis and other orthopedic diseases for thousands of years. However, whether FLL plays an anti-osteoporosis role in promoting the osteogenic differentiation of BMSCs, as well as its active components, targets, and specific molecular mechanisms, has not been fully elucidated.

Fabrication of immobilized bromelain using cobalt phosphate material prepared in deep eutectic solvent as carrier.

The aim of the present work is to fabricate immobilized bromelain based on the specific interaction between the cobalt ions of carrier and the inherent cysteines contained in bromelain molecules. The cobalt phosphate material was prepared as solid support by using choline chloride (ChCl)/betaine-glycerol deep eutectic solvent (DES) as solvent and template for the first time. The Co-P material with lamellate-based structure obtained in the ChCl-glycerol DES at the Co/P ratio of 3:2 showed the best performance for the immobilization of bromelain.

miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1.

Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice.

Scapula chondrosarcoma: A case report.

Rationale: Chondrosarcoma is a malignant mesenchymal tumor originating from cartilage. The pelvis, ribs, femur, and humerus are the most frequently affected sites, and scapula involvement is relatively rare. The aim of the present study was to report a case of chondrosarcoma in the scapula.

Massive rare desmoplastic fibroma of the ilium and ischium in a young adult: A case report.

Rationale: Desmoplastic fibroma (DF) is a rare, locally invasive but benign bone tumor. It represents one of the rarest bone diseases, with an incidence of only 0.11% of all primary bone tumors.

Exogenous spermine preserves mitochondrial bioenergetics via regulating Src kinase signaling in the spinal cord.

Regulation of mitochondrial metabolism is becoming an important target in inhibiting necrosis and apoptosis following secondary spinal cord injury, and physiological compounds that reduce mitochondrial dysfunction are regarded as efficient protective reagents following injury. It has been demonstrated that spermine, a polyamine composed of four primary amines, may be taken up by a mitochondria‑specific uniporter and may preserve mitochondrial bioenergetics, suggesting that it may be important in the pathophysiology of mitochondria. However, the protective mechanism has not yet been definitively clarified.