Safety and contribution of elderly whole blood donors after raising the upper age limit: Hemovigilance data from a Chinese region from 2012 to 2023.

Background: In 2012, China raised the upper age restriction for blood donors from 55 to 60 years old. This study analyzed the impact of raising the upper age restriction on whole blood donor health, contribution to blood supply, and safety of blood.

Methods: The blood collection and donor hemovigilance data of the Chongqing Blood Center from 2012 to 2023 were analyzed to evaluate the safety of elderly blood donors.

Simple analysis of Gaussian pulses and beams in mode-locked lasers.

An ABCDmatrix representation in space and time of a mode-locked laser is presented. The conventional stability concept of Gaussian beams in laser cavities implies, instead of stability, an equilibrium condition, which can be indifferent, stable, or unstable. It is further shown that when beam and pulse evolution in the laser are intertwined, stable mode locking can be the result of evolution of a noise spike in an otherwise unstable (in the conventional sense) cavity.

Selection Behavior and OBP-Transcription Response of Western Flower Thrips, , to Six Plant VOCs from Kidney Beans.

Plant volatile organic compounds (VOCs) are an important link that mediates chemical communication between plants and plants, plants and insects, and plants and natural enemies of insect pests. In this study, we tested the response in the selective behavior of western flower thrips, , to the VOCs of kidney bean, L., to explore their "attraction" or "repellent" effects regarding their application in integrated pest management (i.

Phase Nanoscopy with Correlated Frequency Combs.

This study addresses any sensor based on measuring a physical quantity through the phase of a probing beam. This includes sensing of rotation, acceleration, index change, displacement, fields… While most phase measurements are made by detecting an amplitude change in interfering beams, we detect instead a phase change through a relative frequency shift of two correlated frequency combs. This paper explores the limit sensitivity that this method can achieve, when the combs are generated in an Optical Parametric Oscillator (OPO), pumped synchronously by a train of femtosecond pulses separated by half the OPO cavity round-trip time.

Bibliometric and visual analysis of intestinal ischemia reperfusion from 2004 to 2022.

Background: Intestinal ischemia/reperfusion (I/R) injury is a common tissue-organ damage occurring in surgical practice. This study aims to comprehensively review the collaboration and impact of countries, institutions, authors, subject areas, journals, keywords, and critical literature on intestinal I/R injury from a bibliometric perspective, and to assess the evolution of clustering of knowledge structures and identify hot trends and emerging topics.

Methods: Articles and reviews related to intestinal I/R were retrieved through subject search from Web of Science Core Collection.

Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions.

Although glucose, through pentose phosphate pathway (PPP), is the main source to generate NADPH, solid tumors are often deprived of glucose, hence alternative metabolic pathways to maintain NADPH homeostasis in cancer cells are required. Here, we report that lactate and glutamine support NADPH production via isocitrate dehydrogenase 1 (IDH1) and malic enzyme 1 (ME1), respectively, under glucose-deprived conditions. Isotopic tracing demonstrates that lactate participates in the formation of isocitrate.

Determining the quantitative relationship between glycolysis and GAPDH in cancer cells exhibiting the Warburg effect.

Previous studies have identified GAPDH as a promising target for treating cancer and modulating immunity because its inhibition reduces glycolysis in cells (cancer cells and immune cells) with the Warburg effect, a modified form of cellular metabolism found in cancer cells. However, the quantitative relationship between GAPDH and the aerobic glycolysis remains unknown. Here, using siRNA-mediated knockdown of GAPDH expression and iodoacetate-dependent inhibition of enzyme activity, we examined the quantitative relationship between GAPDH activity and glycolysis rate.

Perturbation of phosphoglycerate kinase 1 (PGK1) only marginally affects glycolysis in cancer cells.

Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.

Lumbopelvic Fixation and Sacral Decompression for U-shaped Sacral Fractures: Surgical Management and Early Outcome.

U-shaped sacral fractures are rare and often difficult to diagnose primarily due to the difficulty in obtaining adequate imaging and the severe associated injuries. These fractures are highly unstable and frequently cause neurological deficits. The majority of surgeons have limited experience in management of U-shaped sacral fractures.

[EVALUATION OF FIBULAR HEAD RESECTION IN PROSTHETIC REPLACEMENT FOR NEOPLASMS OF PROXIMAL TIBIA IN LIMB SALVAGE SURGERY].

Objective: To investigate the effects of fibular head resection in prosthetic replacement for neoplasms of the proximal tibia in limb salvage surgery.

Methods: Between July 1999 and March 2013, 76 patients with neoplasms of the proximal tibia underwent tumor resection, prosthetic replacement, and gastrocnemius medial head flap transfer. Among them, 38 patients underwent fibular head resection (group A) and 38 underwent fibular head preservation (group B).

Piperine inhibits proliferation of human osteosarcoma cells via G2/M phase arrest and metastasis by suppressing MMP-2/-9 expression.

The piperidine alkaloid piperine, a major ingredient in black pepper, inhibits the growth and metastasis of cancer cells both in vivo and in vitro, although its mechanism of action is unclear. Furthermore, its anticancer activity against osteosarcoma cells has not been reported. In this study, we show that piperine inhibited the growth of HOS and U2OS cells in dose- and time-dependent manners but had a weaker effect on the growth of normal hFOB cells.

Induction of a specific CD8+ T-cell response to cancer/testis antigens by demethylating pre-treatment against osteosarcoma.

Conventional non-surgical therapeutic regimens against osteosarcoma are subject to chemoresistance and tumor relapse, and immunotherapy may be promising for this tumor. However, it's hard to find satisfactory epitopes for immunotherapy against osteosarcoma. Cancer/testis antigens (CTAs), such as MAGE-A family and NY-ESO-1, the potential antigens that almost exclusively express in tumor cells and immune-privileged sites, have been found expressed in osteosarcoma also.

Effects of industrial storage on the bioreduction capacity of brewer's yeast.

The effects of industrial storage on the changes of the cell viability and the activities of intracellular alcohol dehydrogenase (ADH) and glucose-6-phosphate dehydrogenase (G6PDH) in brewer's yeast, and the corresponding capacity for the bioconversion of ethyl-3-oxobutanoate (EOB) to ethyl (S)-3-hydroxybutanoate ((S)-EHB), were investigated. The viability of fresh brewer's yeast cells stored in industrial circulating cooling water at 1-2 degrees C showed 4 and 15% drop after the storage of 7 and 15 days, respectively, after which cells died rapidly. The pretreatment of the stored brewer's yeast cells by washing and screening significantly enhanced cell viability during industrial storage.

Promotion of PEM self-humidifying effect by nanometer-sized sulfated zirconia-supported Pt catalyst hybrid with sulfonated poly(ether ether ketone).

A self-humidifying membrane based on low-cost sulfonated poly (ether ether ketone) (SPEEK) hybrid with sulfated zirconia (SO4(2-)/ZrO2, SZ)-supported platinum catalyst (Pt-SZ catalyst) was investigated for fuel cell applications. The SZ particle, a solid-state superacid with hygroscopic and high proton conductivity properties, was employed as the catalyst support. The SPEEK/Pt-SZ self-humidifying membrane was characterized by TEM and SEM coupled with EDX.

Bioconversion of ethyl 4-chloro-3-oxobutanoate by permeabilized fresh brewer's yeast cells in the presence of allyl bromide.

Ethyl(R)-4-chloro-3-hydroxybutanoate ((R)-CHBE) are obtained by cetyltrimetylammonium bromide (CTAB) permeabilized fresh brewer's yeast whole cells bioconversion of ethyl 4-chloro-3-oxobutanoate (COBE ) in the presence of allyl bromide. The results showed that the activities of alcohol dehydrogenase (ADH) and glucose-6-phosphate dehydrogenase (G6PDH) in CTAB permeabilized brewer's yeast cells increased 525 and 7.9-fold, respectively, compared with that in the nonpermeabilized cells and had high enantioselectivity to convert COBE to (R)-CHBE.

An ultrathin self-humidifying membrane for PEM fuel cell application: fabrication, characterization, and experimental analysis.

An ultrathin poly(tetrafluoroethylene) (PTFE)-reinforced multilayer self-humidifying composite membrane (20 microm, thick) is developed. The membrane is composed of Nafion-impregnated porous PTFE composite as the central layer, and SiO2 supported nanosized Pt particles (Pt-SiO2) imbedded into the Nafion as the two side layers. The proton exchange membrane (PEM) fuel cell employing the self-humidifying membrane (Pt-SiO2/NP) turns out a peak power density of 1.

Synthesis and structure-activity relationship exploration of carbon-supported PtRuNi nanocomposite as a CO-tolerant electrocatalyst for proton exchange membrane fuel cells.

A carbon-supported PtRuNi nanocomposite is synthesized via a microwave-irradiated polyol plus annealing synthesis strategy. The catalyst is characterized by transmission electron microscopy, powder X-ray diffraction, energy dispersive spectroscopy, and X-ray photoelectron spectroscopy. The data are discussed with respect to those for the carbon-supported PtRu nanocomposite prepared following the same way.

[Ultrastructure of intervertebral disk in the corresponding area after internal fixation of spinal column].

Objective: To observe ultrastructural changes of the intervertebral disk in the corresponding area after internal fixation of spinal column.

Methods: Twenty-four Japanese big ear rabbits were divided into internal fixation of spinal column group (n=12) and control group (n=12). The internal fixation model was made as follows: The spinous processes and erector spinal muscle were exposed and the T10-L3 spinous processes and the relevant two-side articular processes under the periosteum were isolated.