Clinical imipenem non-susceptible Klebsiella pneumoniae isolates from China: Epidemiology, molecular characterization and in vitro activity of imipenem/relebactam.

Objective: To describe the epidemiological and molecular characterization and in vitro activity of imipenem/relebactam against imipenem non-susceptible (IPMNS) Klebsiella pneumoniae isolates in China.

Methods: K. pneumoniae isolates were collected from 16 sites in 5 regions across China during 2019.

Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates.

Objective: To establish the epidemiological cut-off values (ECOFFs) of sitafloxacin against , and

Methods: We collected 2264 clinical isolates from five different labs located in four cities in China. The minimum inhibitory concentrations (MICs) and inhibition zone diameters of sitafloxacin for all isolates were determined by using the broth microdilution method (BMD) and the disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. MIC ECOFFs were determined using ECOFFinder software, with the 99% calculated ECOFF selected as the initial value.

Role of plasma and blood-cell co-metagenomic sequencing in precise diagnosis and severity evaluation of sepsis, a prospective cohort study in sepsis patients.

Purposes: Sepsis caused great clinical burden all over the world. This study clarified the value of plasma metagenomic next-generation sequencing (p-mNGS) and blood cell mNGS (bc-mNGS) in sepsis diagnosis and evaluation.

Methods: One hundred and fourty-seven blood samples were collected from sepsis patients who met sepsis 3.

Regulatory Mechanisms and Physiological Impacts of Quorum Sensing in Gram-Negative Bacteria.

The Quorum sensing (QS) system is a widely existing communication mechanism, which regulates bacterial community behaviors and the expression of specific genes. The most common pathogenic bacteria in clinical infections are gram-negative bacteria, and QS plays an important regulatory role in the production of virulence factors and development of antibiotic resistance. This article reviews the QS systems of gram-negative bacteria and provides an overview of how they regulate their physiological functions.

Geneticand phenotypic characterization of a novel ST45-K43 carbapenem-resistant strain causing bloodstream infection: a potential clinical threat.

is a common pathogen of nosocomial infection, which can cause pneumonia, urinary tract infection, cystitis, and bloodstream infections (BSIs). Here, we genetically characterize a novel carbapenem-resistant (CRKP) strain recovered from the blood of a 44-year-old male patient with severe acute necrotizing pancreatitis and septic shock in China. The strain is a ST45 with a novel serotype of K43, named 18SHX166.

The key role of iroBCDN-lacking pLVPK-like plasmid in the evolution of the most prevalent hypervirulent carbapenem-resistant ST11-KL64 Klebsiella pneumoniae in China.

Aims: Hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP), coharboring hypervirulence and carbapenem-resistance genes mediated by plasmids, causes infections with extremely high mortality and seriously impacts public health. Exploring the transfer mechanisms of virulence/carbapenem-resistance plasmids, as well as the formation and evolution pathway of hv-CRKP is of great significance to the control of hv-CRKP infections.

Methods: In this study, we identified the predominant clone of hv-CRKP in China and elucidated its genomic characteristics and formation route based on 239 multicenter clinical K.

Effect of ceftazidime-avibactam combined with different antimicrobials against carbapenem-resistant .

Unlabelled: This study aimed to assess the efficacy of ceftazidime-avibactam (CZA) in combination with various antimicrobial agents against carbapenem-resistant (CRKP). We selected 59 clinical CRKP isolates containing distinct drug resistance mechanisms. The minimum inhibitory concentrations (MICs) of meropenem (MEM), colistin (COL), eravacycline (ERA), amikacin (AK), fosfomycin (FOS), and aztreonam (ATM), both individually and in combination with CZA, were tested using the checkerboard method.

[Clinical experience on postoperative balance of hemostasis and antithrombus for patients with hemophilic arthritis after arthroplasty].

Objective: To observe the clinical significance of postoperative personalized antithrombotic therapy for patients with hemophilic arthritis (HA) patients after arthroplasty.

Methods: From September 2005 to October 2013, 11 cases of arthroplasty for hemophilic arthritis in hip and knee total operation 14 times,including 1 case of double knees (calculated as one operation), operation in left knees 6 times, operation in right knees 5 times, 2 in hip. All the patients were male and the age ranged from 23 to 57 years old,with an average of (36.

Preparation and characterization of antimicrobial nano-hydroxyapatite composites.

Deep infection of prosthesis is one of the most frequent complications after joint replacement. One of the most effective ways is to introduce directly some antibiotics in the local site of the surgery. In the present study, an antimicrobial composite has been fabricated using nano-hydroxyapatite particles as carriers for the antimicrobial drug of vancomycin hydrochloride (VAN) and the mixture of oxidation sodium alginate (OSA) and gelatin (GT) as a sticky matrix.

Administration of enoxaparin 24 h after total knee arthroplasty: safer for bleeding and equally effective for deep venous thrombosis prevention.

Background: The purpose of this study was to determine the safety and effectiveness, as assessed by risk of bleeding and incidence of deep venous thrombosis (DVT), of administering delayed low-molecular-weight heparin (LMWH) after total knee arthroplasty.

Methods: A prospective study of 210 consecutive patients undergoing primary unilateral total knee arthroplasty was undertaken. The patients were randomized into two groups: one of which was managed according to a standard LMWH program (LMWH-s group) and the other with delayed LMWH (LMWH-p).

[Short-term curative effects of Tantalum rod treatment in early avascular necrosis].

Objective: To explore the recent clinical curative effect of Tantalum rod in treating the early avascular necrosis.

Methods: From January 2008 to November 2008, the 25 patients (39 hips) with early avascular necrosis accepted tantalum rod placement and included 9 males (11 hips) and 16 females (28 hips) with an average age of 37 years old ranging from 18 to 74 years old. Four patients (6 hips) caused by Alcoholic, 6 patients (8 hips) by hormone, 2 cases (2 hips) by traumatic, 13 cases (23 hips) by idiopathic.

Good short-term outcome of arthroplasty with Wagner SL implants for unstable intertrochanteric osteoporotic fractures.

The purpose of this study was to evaluate the clinical and radiologic results of a consecutive series of Wagner SL arthroplasties in patients with unstable intertrochanteric osteoporotic fractures. Forty-seven consecutive arthroplasties for unstable intertrochanteric fractures using Wagner SL hip stems were studied. All patients had bone mineral density less than -2.

[Total hip arthroplasty for the post-traumatic osteoarthritis after open reduction and internal fixation of acetabular fracture].

Objective: To explore the surgical strategy of traumatic osteoarthritis of the hip joint secondary to the failure of open reduction and internal fixation (ORIF) treatment on acetabular fractures.

Methods: Eighteen cases of traumatic osteoarthritis of the hip joint received total hip arthroplasties from May 2002 to December 2009, who had undergone the surgery of ORIF after acetabular fractures. There were 12 male and 6 female with an average age of 53 years (45 to 66 years).

Effects of patellar resurfacing on contact area and contact stress in total knee arthroplasty.

The objective of this study was to examine the effects of patellar resurfacing on patellofemoral joint contact pressure and contact area in total knee arthroplasty. We tested seven fresh-frozen cadaveric knees using a custom knee jig which permits the simulation of physiologic quadriceps loading. Before patellar resurfacing, the mean peak contact pressure of medial and lateral patellofemoral joints was less than 10 MPa at knee flexion angles of 30 degrees, 60 degrees and 90 degrees, that of medial and lateral patellofemoral joints were 11.

[The effect of rotational alignment of the femoral components on the patellofemoral biomechanics in total knee arthroplasty].

Objective: To study the effect of rotational alignment of the femoral components on the patellofemoral biomechanics in total knee arthroplasty (TKA) demonstrated on autopsy specimens, as the guide for surgeons to choose the correct reference axis for rotational alignment of the femoral components and to reduce the patellofemoral joint complications.

Methods: Select 9 frozen fresh human cadaver knees without gross deformities or instabilities and mount specimens on a patellofemoral joint testing jig connected to a Model 8501 Instron machine (Instron Corporation, Canton, MA). The study simulated the action of squatting from the standing position with the foot firmly planted.