Effects of a bifid triple viable capsule on inflammatory factors and intestinal barrier function in children with abdominal infection.

Objective: To investigate the effects of Bifid Triple Viable (BTV) capsule on inflammatory factors and intestinal barrier function in children with abdominal infection.

Methods: 100 children with abdominal infections were selected. The control group (50 cases) was given conventional treatment, and the observation group (50 cases) was given BTV capsule treatment on the basis of the control group.

A study on the diagnostic value of artificial intelligence combined with a contrast-enhanced ultrasound scoring system in partially cystic thyroid carcinoma.

Objectives: The aim of this study was to investigate the diagnostic value of the contrast-enhanced ultrasound (CEUS) scoring system, artificial intelligence (AI) and the American College of Radiology Thyroid Imaging and Reporting Data System when used by sonographers of different seniority levels individually and in combination for the diagnosis of partial cystic thyroid nodules (PCTNs).

Materials And Methods: A retrospective analysis of conventional ultrasound and CEUS images of enrolled patients was performed, and a CEUS scoring system was established. The sensitivity, specificity, and area under the curve (AUC) of CEUS and AI individually and in combination for diagnosis were compared among sonographers with different seniority levels.

Anti-Fungal Compounds From the Deep-Sea-Derived Tritirachium oryzae-Inhibited Phytophthora nicotianae by Blocking TCA Cycle.

Four new sesquiterpenes (1-4) and 34 known compounds (5-38) were isolated from the deep-sea-derived fungus Tritirachium oryzae. The structures of the new compounds were established by detailed analyses of the nuclear magnetic resonance (NMR), mass spectrometry (MS), optical rotatory dispersion (ORD), and electronic circular dichroism (ECD) data. An in vitro antifungal experiment was conducted against Phytophthora nicotianae, and compound 7 exhibited prominent inhibitory activity with an EC value (5 days) of 18.

Radiomics Combined with ACR TI-RADS for Thyroid Nodules: Diagnostic Performance, Unnecessary Biopsy Rate, and Nomogram Construction.

Rationale And Objectives: To develop a radiomics model with enhanced diagnostic performance, reduced unnecessary fine needle aspiration biopsy (FNA) rate, and improved clinical net benefit for thyroid nodules.

Methods: We conducted a retrospective study of 217 thyroid nodules. Lesions were divided into training (n = 152) and verification (n = 65) cohorts.

Acupuncture improve proteinuria in diabetic kidney disease rats by inhibiting ferroptosis and epithelial-mesenchymal transition.

Objective: To explore the mechanism of acupuncture to relieve diabetic proteinuria in Diabetic Kidney Disease (DKD).

Methods: A total of 10 male Sprague-Dawley rats were randomly selected as the negative control group (NC), and a further 30 rats were fed a high-fat diet (HFD) and intraperitoneal streptozotocin (STZ). The DKD model rats in the model group (DKD) and the acupuncture group (DKD + Acu) were randomly assigned.

USP19 Stabilizes TAK1 to Regulate High Glucose/Free Fatty Acid-induced Dysfunction in HK-2 Cells.

Objective: Obesity-induced kidney injury contributes to the development of diabetic nephropathy (DN). Here, we identified the functions of ubiquitin-specific peptidase 19 (USP19) in HK-2 cells exposed to a combination of high glucose (HG) and free fatty acid (FFA) and determined its association with TGF-beta-activated kinase 1 (TAK1).

Methods: HK-2 cells were exposed to a combination of HG and FFA.

Complete composition analysis of polysaccharides based on HPAEC-PAD coupled with quantitative analysis of multi-components by single marker.

Introduction: Monosaccharide compositions analysis (MCA) is indispensable for structural characterisations and structure-activity relationships of plant polysaccharides.

Objectives: To develop a concise and direct MCA method, we established a quantitative analysis of the multi-monosaccharaides by single marker (QAMS) by high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD) method.

Methodology: A stable and reproducible HPAEC-PAD method for simultaneous determination of aldoses, ketoses and uronic acids (i.

Microwave assisted free radical degradation of Schisandra polysaccharides: Optimization, identification and application.

In order to establish structural-fingerprinting of polysaccharides for improvement of quality assessment, a sample preparation method based on microwave assisted free radical degradation (MFRD) of plant polysaccharides was proposed to produce oligosaccharides and small M polysaccharides. As a case study of Schisandra chinensis and S. sphenanthera fruit polysaccharides (SCP and SSP), the MFRD condition (i.

[Effects of simulated insect herbivory on defense traits of ].

To understand mechanisms underlying invasion and its responses to simulated insect herbivory, individuals of were treated with different concentrations of methyl jasmonate (MeJA) before blooming. We measued plant height, abundance of leaves and inflorescences, biomass, specific leaf area, trichome density, condensed tannins, total polyphenols, and flavonoids in leaves and inflorescences. The growth and reproduction parameters of treated with 5 mmol·L MeJA were not significantly different from those of control, higher than those of control when treated with 10 mmol·L MeJA, with significant difference except plant height, and declined when treated with 20 mmol·L MeJA.

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of .

The aim of this work was to extract, isolate, and purify polysaccharides from the heads of and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from the heads of . The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid-liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.

Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum.

The aim of this work is to characterize the primary structure and physicochemical properties of natural polysaccharides (GLP) and degraded polysaccharides (GLP) from Ganoderma lucidum, and evaluate their hypolipidemic and antioxidant activities. The results of particle size distribution and scanning electron microscopy (SEM) showed that Ganoderma lucidum polysaccharides were effectively degraded by ultrasonic method. GLP was composed of the same monosaccharide units as GLP but with different molar ratios.

12-Acetyloxyperforatin, a New Limonoid from .

A new A, D-seco limonoid, named 12-acetyloxyperforatin (), along with three known ones, were isolated from the leaves of . Their structures were elucidated on the basis of spectroscopic analysis, including extensive NMR techniques and computational modelling. These compounds showed no inhibitory activity against the 11-HSD1 enzyme.

16-nor Limonoids from Harrisonia perforata as promising selective 11β-HSD1 inhibitors.

Two new 16-nor limonoids, harperspinoids A and B (1 and 2), with a unique 7/5/5/6/5 ring system, have been isolated from the plant Harrisonia perforate together with a known one, Harperforin G (3). Their structures were elucidated by NMR spectroscopy, X-ray diffraction analysis and computational modelling. Compound 1 exists as polymorphic crystals.

Ionic Adsorption and Desorption of CNT Nanoropes.

A nanorope is comprised of several carbon nanotubes (CNTs) with different chiralities. A molecular dynamic model is built to investigate the ionic adsorption and desorption of the CNT nanoropes. The charge distribution on the nanorope is obtained by using a modified gradient method based on classical electrostatic theory.

Anti-candidal activity of a novel peptide derived from human chromogranin A and its mechanism of action against .

species ( spp.) are important fungal pathogens, which cause numerous clinical diseases associated with significant mortality and morbidity in healthcare settings. In our previous study, we identified a recombinant peptide, chromogranin A (CGA)-N46, corresponding to the N-terminal Pro31-Gln76 sequence of human CGA, that exhibited antifungal activity against .

miRNA-548p suppresses hepatitis B virus X protein associated hepatocellular carcinoma by downregulating oncoprotein hepatitis B x-interacting protein.

Aim: miR-548p is a recently identified and poorly characterized miRNA. However, its role of miR-548p in tumorigenesis and progression remains poorly understood. Here, we aimed to investigate the biofunction of miR-548p in hepatocellular carcinogenesis.

[Mechanism of biological actions of quercetin based on biomolecular network].

The mechanism of biological actions of quercetin was studied by using metabolomic method and biomolecular network. HPLC-MS was used to analyze the serum metabolome in rats of blank group and quercetin administration group rats, and MS data were processed by MATLAB software. With multivariate statistical analysis of serum metabolite profiles, a clear separation among blank group and quercetin administration group was achieved, potential biomarkers were selected according to the parameters of variable importance in the projection (VIP) and identified according to MS information and database retrieval.

The HIF-2alpha dependent induction of PAP and adenosine synthesis regulates glioblastoma stem cell function through the A2B adenosine receptor.

Glioblastomas are lethal tumors characterized by malignant proliferation and recurrence promoted partly by glioblastoma stem cells (GSCs). GSCs are known to be regulated by hypoxia, but the mechanisms involved in this regulation are not fully understood. We now demonstrate that hypoxia-inducible factor HIF2α and prostatic acid phosphatase (PAP) are preferentially expressed in hypoxic GSCs in comparison with non-stem tumor cells and normal neural stem cells and that PAP is regulated by HIF2α.

Electrochemical enzyme-linked immunosorbent assay (ELISA) for α-fetoprotein based on glucose detection with multienzyme-nanoparticle amplification.

Since glucose biosensors are one of the most popular and widely used point-of-care testing devices, a novel electrochemical enzyme-linked immunosorbent assay (ELISA) for protein biomarkers has been developed based on a glucose detection strategy. In this study, α-fetoprotein (AFP) was used as the target protein. An electrochemical ELISA system was constructed using anti-AFP antibodies immobilized on microwell plates as the capture antibody (Ab1) and multi-label bioconjugates as signal tracer.

A microstructural study of individual nacre tablet of Pinctada maxima.

Nacre tablets from the shell of Pinctada maxima were studied with SEM, TEM and STEM. The systematic nanolath morphology on the (001) surface of nacre tablets was observed after acidic etching and mechanical polishing. The nanolaths were along the [100] crystallographic orientation of aragonite crystal.

Preparation and self-sterilizing properties of Ag@TiO2-styrene-acrylic complex coatings.

In this study, we report a simple and cost-effective method for self-sterilized complex coatings obtained by Ag@TiO2 particle incorporation into styrene-acrylic latex. The Ag@TiO2 particles were prepared via a coupling agent modification process. The composite latices characterized by transmission electron microscopy (TEM) study were highly homogeneous at the nanometric scale, and the Ag@TiO2 particles were well dispersed and exhibited an intimate contact between both the organic and inorganic components.

Three novel β-carboline alkaloids from Gelsemium elegans.

Three novel β-carboline alkaloids characterized with a unique degraded monoterpenoid moiety, gelebolines A-C (1-3), together with 11 known alkaloids of different types, were isolated from the roots of Gelsemium elegans. The structures of new alkaloids were established on the basis of analysis of spectroscopic data. It is the first example for report of β-carboline from genus Gelsemium (Loganiaceae).

[Study on the effects of gonadotropin-releasing hormone analogues in the inhibition of ovarian cancer transplanted tumors and in the protection of ovarian function after chemotherapy on nude mice].

Objective: To investigate the influence of gonadotropin-releasing hormone (GnRH) analogues on ovarian cancer and ovarian function in vivo.

Methods: ES-2 cells were cultured and xenotransplanted into 36 nude mice, which were divided into 6 groups: normal saline (NS) group: NS 0.1 ml/day subcutaneous injection, and then NS 0.