Ankle morphometry based on CT in Chinese population.

Background: Total ankle arthroplasty (TAA) is a surgical intervention for end-stage ankle arthritis. However, existing ankle prostheses continue to exhibit certain limitations, and there remains a need to develop prostheses that better conform to the ankle's anatomic characteristics. The objective of this study was to evaluate ankle morphometric data to establish an anatomical foundation for designing improved ankle prostheses.

Tandem ssDNA in neutrophil extracellular traps binds thrombin and regulates immunothrombosis.

Neutrophils release neutrophil extracellular traps (NETs) to neutralize infections, a process that also contributes to immunothrombosis. While beneficial in localized infections, excessive NET formation can lead to widespread coagulopathy and organ failure. While the roles of NET-associated proteins such as histones in immunothrombosis are well characterized, NET-derived DNAs are much less known.

[5]Helicene-Embedded Cycloparaphenylene Nanohoops with Möbius Topology: Synthesis, Photophysical Properties, and Aromaticity.

The relationship between Möbius topology and aromaticity still remains elusive, which is largely due to the related synthetic challenges and, further, the scarcity in both the quantity and the diversity of the constructed Möbius systems. In this work, we report the synthesis of [4n]Möbius conjugated all-carbon nanohoops ([5]H-[7,8]CPPs) by utilizing a [5]helicene unit as a hidden writhe and a masked aromatic unit to overcome the strain inherited from Möbius topology. X-ray analyses reveal that [5]H-[7,8]CPPs contain a [5]helicene moiety and an oligoparaphenylene unit, clearly exhibiting Möbius topology.

Decoding Potassium Homeostasis in Cancer Metastasis and Drug Resistance: Insights from a Highly Selective DNAzyme-Based Intracellular K Sensor.

Potassium ions (K) within the tumor microenvironment, along with dysregulation of K channels, play critical roles in supporting cancer cell survival and preventing their elimination. Directly monitoring changes in K homeostasis within cancer cells is invaluable for understanding these processes. However, achieving high selectivity over other biological metal ions, a detection dynamic range that aligns with intracellular K levels, and broad accessibility to research laboratories remain technically challenging for current K imaging probes.

Research on scraper conveyor load prediction method based on wavelet transform and BP neural network.

Scraper conveyor load prediction is crucial to realize the cooperative speed regulation of coal mining machine and scraper conveyor. In the synthesized mining face, due to the uncertainty of the coal fall, the load of the scraper conveyor fluctuates due to the change of the coal load, which shows a strong nonlinearity and non-smoothness, leading to the difficulty of prediction. To solve this problem, this paper proposes a BP neural network model combined with wavelet transform for scraper conveyor current prediction.

Highly selective DNA aptamer sensor for intracellular detection of coenzyme A.

Detecting Coenzyme A (CoA) in cells is vital for understanding its role in metabolism. DNA aptamers, though widely used for monitoring many other molecules, have not been effective for CoA detection, as previous attempts at obtaining DNA aptamers for CoA using SELEX resulted in aptamers that only recognize the adenine moiety of CoA. This "tyranny" of adenine dominating in SELEX has, therefore, hampered the SELEX of aptamers specific for CoA.

[Causal Relationship Between Components of Metabolic Syndrome and Endometrial Carcinoma].

Objective: To investigate the causal associations between components of metabolic syndrome (MetS) and endometrial carcinoma using Mendelian randomization (MR).

Methods: Data mining of the Genome-Wide Association Studies (GWAS) database was performed, with the exposure factors being MetS components (lipids, blood pressure, blood glucose, and obesity) and the outcome factor being endometrial carcinoma. MR analyses were performed with the help of regression models, including MR-Egger method, weighted median method, and inverse variance weighted (IVW) method, and the causal relationship between MetS components and endometrial carcinoma was evaluated by odds ratio (OR).

Synthetic transmembrane DNA receptors enable engineered sensing and actuation.

In living organisms, cells synergistically couple cascade reaction pathways to achieve inter- and intracellular signal transduction by transmembrane protein receptors. The construction and assembly of synthetic receptor analogs that can mimic such biological processes is a central goal of synthetic biochemistry and bionanotechnology to endow receptors with user-defined signal transduction effects. However, designing artificial transmembrane receptors with the desired input, output, and performance parameters are challenging.

Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells.

Glycosylated RNAs (glycoRNAs) have recently emerged as a new class of molecules of substantial interest owing to their potential roles in cellular processes and diseases. However, studying glycoRNAs is challenging owing to the lack of effective research tools including, but not limited to, imaging techniques to study the spatial distribution of glycoRNAs. Recently, we reported the development of a glycoRNA imaging technique, called sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay (ARPLA), to visualize sialic acid-containing glycoRNAs with high sensitivity and specificity.

Osteochondral lesions of the talus with small cysts may not affect the outcome of arthroscopic microfracture: A systematic review and meta-analysis.

Background: The optimal treatment of Osteochondral lesion of the talus (OLT) for subchondral bone cysts (SBCs) has not been finalized. The purpose of this systematic review and meta-analysis was to define whether OLT with small SBCs will affect the clinical outcomes of OLTs after arthroscopic microfracture.

Methods: We searched the Embase, Cochrane Library and PubMed databases up to May 13, 2024 for eligible comparative studies.

On-Site Portable Lithium Detection in Mining and Recycling Industries Based on a DNAzyme Fluorescent Sensor.

The global demand for lithium has soared in recent years due to the wide use of lithium batteries. To meet this demand, we herein report developing novel on-site sample preparation methods for the extraction of Li from relevant materials, including brine water, spodumene rock, as well as lithium-ion battery electrodes, and a DNAzyme-based fluorescent sensor for sensitive and robust detection of Li in these samples down to 1.4 mM (10 ppm) using a portable fluorometer.

Vehicular Mini-LED backlight display inspection based on residual global context mechanism.

Mini-LED backlight has emerged as a promising technology for high performance LCDs, yet the massive detection of dead pixels and precise LEDs placement are constrained by the miniature scale of the Mini-LEDs. The high-resolution network (Hrnet) with mixed dilated convolution and dense upsampling convolution (MDC-DUC) module and a residual global context attention (RGCA) module has been proposed to detect the quality of vehicular Mini-LED backlights. The proposed model outperforms the baseline networks of Unet, Pspnet, Deeplabv3+, and Hrnet, with a mean intersection over union (Miou) of 86.

Small-molecule natural product sophoricoside reduces peripheral neuropathic pain via directly blocking of NaV1.6 in dorsal root ganglion nociceptive neurons.

Peripheral neuropathic pain poses a significant global health challenge. Current drugs for peripheral neuropathic pain often fall short in efficacy or come with severe side effects, emphasizing the critical need for the development of highly effective and well-tolerated alternatives. Sophoricoside (SOP) is a nature product-derived isoflavone that possesses various pharmacological effects on inflammatory and neuropathy diseases.

Involuntary and voluntary processes compete for entering the focus of attention of working memory.

Representations in the focus of attention (FoA) of working memory (WM) have the highest activation state and processing privilege among representations in WM. There are two distinct processes for representations entering the FoA: involuntary and voluntary. The former is an automatic attentional response to stimuli, while the latter is directed by the central executive.

Limited impact of the sidewall effect in dependence of temperature for InGaN-based blue micro-LEDs grown on a silicon substrate.

The electroluminescence (EL) properties of InGaN-based micro-LEDs grown on a silicon substrate are investigated in this Letter to reveal the dominant mechanism in dependence on different temperatures and dimensions. The invalidation of sidewall nonradiative recombination and the impact of localization-induced carrier tunneling on the external quantum efficiency (EQE) are analyzed systematically to realize high performance silicon-based micro-LEDs. Microscopic EL mapping exhibits that the localized carriers in the silicon-grown micro-LED mainly recombine in the central region of mesa.

[Exploring the Causal Relationship Between Coagulation Function and Gestational Diabetes Mellitus Through Mendelian Randomization].

Objective: To explore the causal association between coagulation function, including von Willebrand factor (vWF), a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13 (ADAMTS13), activated partial thromboplastin time (aPTT), coagulation factor Ⅷ (FⅧ), coagulation factor Ⅺ (FⅪ), coagulation factor Ⅶ (FⅦ), coagulation factor Ⅹ (FⅩ), endogenous thrombin potential (ETP), plasminogen activator inhibitor-1 (PAI-1), protein C, and plasmin, and gestational diabetes mellitus (GDM) using two-sample two-way Mendelian randomization (MR), and to provide genetic evidence for the association between coagulation function and the pathogenesis of GDM.

Methods: The IEU OpenGWAS database was accessed using the R package TwoSampleMR (v 0.5.

Carbenoid-involved reactions integrated with scaffold-based screening generates a Nav1.7 inhibitor.

The discovery of selective Nav1.7 inhibitors is a promising approach for developing anti-nociceptive drugs. In this study, we present a novel oxindole-based readily accessible library (OREAL), which is characterized by readily accessibility, unique chemical space, ideal drug-like properties, and structural diversity.

Investigation of optical polarization characteristics of ultraviolet-C AlGaN multiple quantum wells by angle-resolved cathodoluminescence.

AlGaN-based ultraviolet-C (UV-C) light-emitting diodes (LEDs) face challenges related to their extremely low external quantum efficiency, which is predominantly attributed to the remarkably inadequate transverse magnetic (TM) light extraction efficiency (LEE). In this study, we employ angle-resolved cathodoluminescence (ARCL) spectroscopy to assess the optical polarization of (0001)-oriented AlGaN multiple quantum well (MQW) structures in UV-C LEDs, in conjunction with a focused ion beam and scanning electron microscopy (FIB/SEM) system to etch samples with various inclination angles (θ) of sidewall. This technique effectively distinguishes the spatial distribution of TM- and transverse electric (TE)-polarized photons contributing to the luminescence of the MQW structure.

Spatially resolved current density distribution in GaN-based flip-chip green mini-LEDs by microscopic hyperspectral imaging and modified two-level modeling.

A modified two-level model is proposed to study the spatially resolved current density distribution of GaN-based green miniaturized light-emitting diodes (mini-LEDs), combining with microscopic hyperspectral imaging. We found that the spatially resolved current density distribution reveals both the radiative and non-radiative recombination mappings, which can also be provided separately by this model. In addition, higher current density is not necessarily correlated with higher photon emission, especially for the regions around the electrode edges, where the high current density suggests current crowding and defect-related non-radiative recombination.

Asymmetric Carbene-Alkyne Metathesis-Mediated Cascade: Synthesis of Benzoxazine Polychiral Polyheterocycles and Discovery of a Novel Pain Blocker.

This study introduces a novel approach for synthesizing Benzoxazine-centered Polychiral Polyheterocycles (BPCPHCs) via an innovative asymmetric carbene-alkyne metathesis-triggered cascade. Overcoming challenges associated with intricate stereochemistry and multiple chiral centers, the catalytic asymmetric Carbene Alkyne Metathesis-mediated Cascade (CAMC) is employed using dirhodium catalyst/Brønsted acid co-catalysis, ensuring precise stereo control as validated by X-ray crystallography. Systematic substrate scope evaluation establishes exceptional diastereo- and enantioselectivities, creating a unique library of BPCPHCs.

Nav1.7 Modulator Bearing a 3-Hydroxyindole Backbone Holds the Potential to Reverse Neuropathic Pain.

Chronic pain is a growing global health problem affecting at least 10% of the world's population. However, current chronic pain treatments are inadequate. Voltage-gated sodium channels (Navs) play a pivotal role in regulating neuronal excitability and pain signal transmission and thus are main targets for nonopioid painkiller development, especially those preferentially expressed in dorsal root ganglial (DRG) neurons, such as Nav1.